Anti-CD40 Antibody

Boster Bio Anti-CD40 Antibody catalog # PA1019. Tested in WB applications. This antibody reacts with Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: PA1019
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-CD40 Antibody

See all CD40/TNFRSF5 products

SKU/Catalog Number

PA1019

Size

100μg/vial

Form

lyophilized

Description

Boster Bio Anti-CD40 Antibody catalog # PA1019. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1019)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD40 (22-36aa, QCVTCSDKQYLHDGQ).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1019 is reactive to Cd40 in Mouse

Applications

PA1019 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cd40 (Source: Uniprot.Org, NCBI)

Uniprot ID

P27512

Gene ID

21939

Gene Name

Cd40

Full Name

Tumor necrosis factor receptor superfamily member 5

Weight

32.093kDa

Alternative Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 Cd40|AI326936, Bp5, Bp50, GP39, HIGM1, IGM, IMD3, T-BAM, TRAP, Tnfr, Tnfrsf5, p5, p50|CD40 antigen|tumor necrosis factor receptor superfamily member 5|B-cell surface antigen CD40|CD40L receptor|T-cell differentiation antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd40, check out the Cd40 Infographic

Cd40 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1019 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...

Peng Yd, Cheng Lx, Zeng Qt, Shi Y, Bao Xx, Mao Xb, Ji Qw, Li Sn, Guo M, Liang Zs, Wu Tc. Cell Biol Int. 2010 Mar 24;34(5):447-53. Doi: 10.1042/Cbi20090301. The Role Of Rantes As A Crucial Downstream Cytokine In Calcineurin-Dependent Vsmc Apoptosis...

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4 Customer Q&As for Anti-CD40 Antibody

Question

We are currently using anti-CD40 antibody PA1019 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The anti-CD40 antibody (PA1019) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-13

Question

Could I conjugate or label this antibody? Keywords: conjugation, fluorescent dye, antibody labeling, fluorescence labeling

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

It is not recommendeded to label the antibody because it contains BSA. Please contact us at [email protected] for BSA-free antibody products.

Boster Scientific Support

Answered: 2020-01-30

Question

We have observed staining in mouse ovary. Do you have any suggestions? Is anti-CD40 antibody supposed to stain ovary positively?

G. Johnson

Verified customer

Asked: 2013-08-02

Answer

According to literature ovary does express CD40. According to Uniprot.org, CD40 is expressed in left coronary artery, kidney, ovary, leukocyte, lymphoma urinary bladder carcinoma, among other tissues. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161

Boster Scientific Support

Answered: 2013-08-02

Question

My team were well pleased with the WB result of your anti-CD40 antibody. However we have observed positive staining in left coronary artery isoform i: cell membrane using this antibody. Is that expected? Could you tell me where is CD40 supposed to be expressed?

K. Bhatt

Verified customer

Asked: 2013-04-30

Answer

From literature, left coronary artery does express CD40. Generally CD40 expresses in isoform i: cell membrane. Regarding which tissues have CD40 expression, here are a few articles citing expression in various tissues:
Lymphoma, and Urinary bladder carcinoma, Pubmed ID: 2463309, 15340161

Boster Scientific Support

Answered: 2013-04-30

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1019
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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