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Product Info Summary
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Boster Bio Anti-CD40 Antibody catalog # PA1019. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1019)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD40 (22-36aa, QCVTCSDKQYLHDGQ).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1019 is reactive to Cd40 in Mouse
PA1019 is guaranteed for WB Boster Guarantee
Background of CD40/TNFRSF5
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Anti-CD40 antibody, PA1019, Western blotting
Lane 1: Recombinant Mouse CD 40 Protein 10ng
Lane 2: Recombinant Mouse CD 40 Protein 5ng
Lane 3: Recombinant Mouse CD 40 Protein 2.5ng
Gene/Protein Information For Cd40 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 5
B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 Cd40|AI326936, Bp5, Bp50, GP39, HIGM1, IGM, IMD3, T-BAM, TRAP, Tnfr, Tnfrsf5, p5, p50|CD40 antigen|tumor necrosis factor receptor superfamily member 5|B-cell surface antigen CD40|CD40L receptor|T-cell differentiation antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd40, check out the Cd40 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1019 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng Hx, Li Hn, Geng Js, Ohe R, Yu Xy, E Xq, Ye F, Yang Sr, Kato T, Zhang L, Ishida A, Ohta N, Jin Xm, Kakehata S, Geng Js, Yamakawa M. Transl Res. 2015 Sep;166(3):281-91. Doi: 10.1016/J.Trsl.2015.04.004. Epub 2015 Apr 17. Decreased Expression Of ...
Peng Yd, Cheng Lx, Zeng Qt, Shi Y, Bao Xx, Mao Xb, Ji Qw, Li Sn, Guo M, Liang Zs, Wu Tc. Cell Biol Int. 2010 Mar 24;34(5):447-53. Doi: 10.1042/Cbi20090301. The Role Of Rantes As A Crucial Downstream Cytokine In Calcineurin-Dependent Vsmc Apoptosis...
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