Cd40 antibody (PB10052) performs well in ELISA, Flow Cytometry, IHC, ICC, WB and shows reactivity with Human, Mouse.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 5 Publications
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Designing a Western blot for CD40? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CD40 Western Blot Guide

Anti-CD40 Antibody Picoband®

Cd40 antibody

Reactive SpeciesHostApplication
Human, MouseRabbitELISA, Flow Cytometry, IHC, ICC, WB

SKU: PB10052

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Quantity: 1

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Tested Applications

Cd40 antibody was positively detected by WB in:

mouse spleen tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Cd40 antibody was positively detected by IHC in:

mouse lymphaden tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Cd40 antibody was positively detected by FC in:

U937 cell

Recommend Dilution

Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
Western blot
0.1-0.5μg/ml
Flow Cytometry
1-3μg/1x106 cells

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.

Reactive Data

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WB
IHC
ICC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Human
Recommend
Recommend

Product Information

Product Name
Anti-CD40 Antibody Picoband®
View all CD40 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD40/TNFRSF5 recombinant protein (Position: L20-R193). Mouse CD40/TNFRSF5 shares 60.7% amino acid (aa) sequence identity with human CD40/TNFRSF5.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight

32.1 kDa

Background of Cd40
Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Cite This Product
Anti-CD40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10052)

Protocols

Western Blot protocol for Cd40 antibody
Immunohistochemistry (IHC) protocol for Cd40 antibody
Flow Cytometry (FC) protocol for Cd40 antibody

Publications

Application:
Species:
Dendritic cell-based biomimetic nanoparticles for foot-and-mouth disease induce robust cellular immunity
Published: 2024-09-25
Journal: ANTIVIRAL RESEARCH
Application: WB,IF
Impact Factor: 4.50
Species: Mouse
PMID: 39332536
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
Decreased expression of follicular dendritic cell–secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy
Published: 2015-04-17
Journal: Translational Research
Application: IHC
Impact Factor: 5.03
Species: Human
PMID: 25953661
VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma
Published: 2014-02-06
Journal: Reproductive Biology and Endocrinology
Application: IHC
Impact Factor: 2.41
Species: Mouse
PMID: 24502459
Coxsackievirus B3 infection promotes generation of myeloid dendritic cells from bone marrow and accumulation in the myocardium
Published: 2009-08-04
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IHC
Impact Factor: 2.16
Species: Mouse
PMID: 19664723

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Product Promise

We promise Anti-CD40 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PB10052
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 27, 2026