Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ0702
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all CD40/TNFRSF5 products

SKU/Catalog Number

EZ0702

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0702)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0702 is reactive to CD40 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EZ0702 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0702-CA Rabbit anti- human CD40 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0702-DA Biotinylated goat anti- human CD40 polyclonal antibody (Detection Antibody) 500 µl, 5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0702-ST Lyophilized recombinant human CD40 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.010.0770.1470.2210.4190.9971.622.394

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)93325538101357529
Standard deviation4.819.228.56.926.432.8
CV (%)5.2%5.9%5.3%6.8%7.4%6.2%

Gene/Protein Information For CD40 (Source: Uniprot.Org, NCBI)

Uniprot ID

P25942

Gene ID

958

Gene Name

CD40

Full Name

Tumor necrosis factor receptor superfamily member 5

Weight

30.619kDa

Alternative Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 CD40 Bp50, CDW40, TNFRSF5, p50 CD40 molecule tumor necrosis factor receptor superfamily member 5|B cell surface antigen CD40|B cell-associated molecule|CD40 molecule, TNF receptor superfamily member 5|CD40L receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD40, check out the CD40 Infographic

CD40 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD40: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

Has anybody tested this EZ0702 antibody to measure dog CD40?

Verified Customer

Verified customer

Asked: 2016-03-23

Answer

Unfortunately, the antibody EZ0702 has not been tested in canine samples and its homology has about 70% only with the human and canine sequences.

Boster Scientific Support

Answered: 2016-03-23

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