Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse;Rat.
Anti-BOB1/POU2AF1 Antibody Info At A Glance
GM-CSFReceptor Alpha (CSF2RA) is the alpha subunit of the heterodimeric receptor forcolony stimulating factor 2, a cytokine which controls the production,differentiation, and function of granulocytes and macrophages. CSFR2 is also amember of the cytokine family of receptors. In addition, this gene is found inthe pseudoautosomal region (PAR) of the X and Y chromosomes. Multipletranscript variants encoding various isoforms have been found for this gene, whilesome of the isoforms being membrane-bound and others being soluble. Diseasesassociated with CSF2RA include surfactant metabolism dysfunction, pulmonary 4,and csf2ra-related pulmonary surfactant metabolism dysfunction.
|Product Name||Anti-BOB1/POU2AF1 Antibody
See all BOB1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for BOB1/POU2AF1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. BOB1/POU2AF1 information: Molecular Weight: 27692 MW; Subcellular Localization: Nucleus; Tissue Specificity: B-cell specific.|
|Cite This Product||Anti-BOB1/POU2AF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1506)|
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|Anti-BOB1/POU2AF1 Antibody may replace the following items: sc 130918|sc 23932|sc 23932 X|sc 25402|sc 25402 X|sc 365595|sc 365595 X|sc 56817|sc 955|sc 955 X.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1(238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Pou2af1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||18985|
|Species Of This Entry||Mouse|
|Protein Name||POU domain class 2-associating factor 1|
|Alternative Names||BOB1|B-cell-specific coactivator OBF-1; BOB-1; OBF-1; OBF1BOB1; OCAB; OCA-B; OCT-binding factor 1; POU class 2 associating factor 1; POU domain class 2, associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1|
|Post Tranlational Modifications||Phosphorylation; Myristoylation; Deamination; Deacetylation; Methylation|
|Gene Location||9|9 A5.3, on Chromasome 9, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Pou2af1 (Source: Uniprot.org, NCBI)
|Protein Function||Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.|
|Tissue Specificity||B-cell specific.|
|Related Diseases||Lymphoma; Hodgkin Disease; B-cell Lymphomas; Neoplasms; Classical Hodgkin's Lymphoma; Diffuse Large B-cell Lymphoma; Leukemia; Nodular Lymphocyte Predominant Hodgkin Lymphoma; Lymphoma, Non-hodgkin; Malignant Neoplasms; Chromosomal Translocation; Plasmacytoma; Ki-1+ Anaplastic Large Cell Lymphoma; T-cell Lymphoma; Multiple Myeloma; Cell Transformation, Neoplastic; Autoimmunity; Burkitt Lymphoma; Hematologic Neoplasms; Malignant Paraganglionic Neoplasm|
|Related Pathways||Cell Development; Germinal Center Formation; Cell Differentiation; B Cell Differentiation; Immune Response; Cell Maturation; Pathogenesis; Cell Activation; B Cell Activation; Immunoglobulin Production; Localization; Secretion; Cell Cycle; Cell Proliferation; Methylation; Lymphocyte Differentiation; Cell Adhesion; Mature B Cell Differentiation; Gene Silencing; Dna Recombination|
|Background||POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.|
|Scientific References||PMID: 8530384 by Pfisterer P., et al. Functional characterization of the murine homolog of the B cell- specific coactivator BOB.1/OBF.1.|
PMID: 8657574 by Schubart D.B., et al. Gene structure and characterization of the murine homologue of the B cell-specific transcriptional coactivator OBF-1.
Our Boster Quality Guarantee for Anti-BOB1/POU2AF1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane: Rat Spleen Tissue Lysate
IHC(P): Rat Tonsil Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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