Mouse IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®

IL-23R ELISA kit for Mouse

Mouse IL-23R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il23r in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1613
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®

View all IL-23R ELISA kits

SKU/Catalog Number

EK1613

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-23R ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il23r in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-23R/Interleukin-23 receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1613)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-D372

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1613 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1613 is reactive to IL23R in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1613 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-23R

Interleukin-23 subunit alpha is a protein that in humans is encoded by the IL23A gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Kit Components

Catalog Number Description Quantity
EK1613-CAP Anti-Mouse IL23R Pre-coated 96-well strip microplate 1
EK1613-ST Mouse IL23R Standard 2 vials, 10 ng/tube
EK1613-DA Mouse IL23R Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0030.0880.1430.2720.5761.0091.5672.024

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-23R, Dilution ratio of 1:1, concentration in serum and plasma is around 600 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-23R/Interleukin-23 receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12963329841226312799
Standard deviation6.4547.47179.046.5847.95201.52
CV (%)5%7.5%6%5.4%7.6%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-23R in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11291171381291287.465.8%
Sample 263358865460361925.674.1%
Sample 329842683305828952905140.564.8%
*number of samples for each test n=16.

Gene/Protein Information For IL23R (Source: Uniprot.Org, NCBI)

Gene Name

IL23R

Full Name

Interleukin-23 receptor

Weight

71.722kDa

Superfamily

type I cytokine receptor family

Alternative Names

IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor IL23R interleukin 23 receptor interleukin-23 receptor|IL-23 receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL23R, check out the IL23R Infographic

IL23R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL23R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1613 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response

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7 Customer Q&As for Mouse IL-23R/Interleukin-23 receptor ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

R. Garcia

Verified customer

Asked: 2020-09-24

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-24

Question

Q: What is the optimal O.D. value for IL-23R ELISA kit? I used your IL-23R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-23R even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-23R ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-23R that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-04-24

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

B. Edwards

Verified customer

Asked: 2018-10-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-10-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

A. Dayal

Verified customer

Asked: 2018-04-01

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-01

Question

Q: if the enzyme conjugated IL-23R antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-23R antigen?

Verified Customer

Verified customer

Asked: 2017-08-18

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-08-18

Question

Q: can I use citrate plasma as samples in Mouse IL-23R Picokine® ELISA Kit (Catalog # EK1613)?

Verified Customer

Verified customer

Asked: 2016-12-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-23R causing degradation of its protein structure. IL-23R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-23R ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-12-15

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2014-06-14

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-06-14

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