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Product Info Summary
| SKU: | A00026-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-c-Myc/MYC Antibody Picoband®
SKU/Catalog Number
A00026-1
Size
100 μg/vial
Form
Lyophilized
Description
MYC is a transcription factor that drives programs for cell growth, proliferation, and metabolic reprogramming, making it a canonical oncogenic node and a common readout in cell-cycle/metabolic state studies (context dependent). Assay context: antibody tested for ELISA, flow cytometry, and WB across human/mouse/rat. Often interpreted with tumor suppressor and cell-cycle control markers such as TP53 and ubiquitin-ligase machinery components like ANAPC2 (APC2) to relate MYC-high states to checkpoint and mitotic progression phenotypes (putative co-panel).
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-c-Myc/MYC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00026-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human c-Myc/MYC recombinant protein (Position: N9-A439).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00026-1 is reactive to MYC in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
48.8 kDa
Background of MYC
MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the myc family of transcription factors. The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00026-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Raji whole cell, human K562 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Western blot analysis of C-Myc/MYC using anti-C-Myc/MYC antibody (A00026-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Myc/MYC antigen affinity purified polyclonal antibody (Catalog # A00026-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Myc/MYC at approximately 65 kDa. The expected band size for C-Myc/MYC is at 65 kDa.
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Flow Cytometry analysis of THP-1 cells using anti-C-Myc/MYC antibody (A00026-1).
Overlay histogram showing THP-1 cells stained with A00026-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Myc/MYC Antibody (A00026-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-c-Myc/MYC Antibody Picoband® (A00026-1)
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