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- Table of Contents
MYC antibody (A00026-1) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of THP-1 cells using anti-C-Myc/MYC antibody (A00026-1).
Overlay histogram showing THP-1 cells stained with A00026-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Myc/MYC Antibody (A00026-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A00026-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Myc/MYC Antibody (A00026-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-c-Myc/MYC Antibody Picoband®
MYC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
MYC antibody was positively detected by WB in:
human Raji whole cell, human K562 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MYC antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-c-Myc/MYC Antibody Picoband®
View all MYC Antibodies
View all MYC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human c-Myc/MYC recombinant protein (Position: N9-A439).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
48.8 kDa
Background of MYC
MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the myc family of transcription factors. The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Cite This Product
Anti-c-Myc/MYC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00026-1)
Protocols
Western Blot protocol for MYC antibody
Flow Cytometry (FC) protocol for MYC antibody
Publications
Application:
Species:
Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents
Published: 2023-03-08
Journal: BIOORGANIC & MEDICINAL CHEMISTRY
Application: WB
Impact Factor: 3.50
Species: Human
PMID: 36906964
Aptamer-assisted superparamagnetic iron oxide nanoparticles as multifunctional drug delivery platform for chemo-photodynamic combination therapy
Published: 2019-06-19
Journal: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Application: WB
Impact Factor: 2.47
Species: Human
PMID: 31218573
Long non-coding RNA LINC01503 predicts worse prognosis in glioma and promotes tumorigenesis and progression through activation of Wnt/β-catenin signaling
Published: 2019-02-28
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 2.72
Species: Human
PMID: 30840283
The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer
Published: 2017-07-05
Journal: Journal of Cancer
Application: WB
Impact Factor: 2.92
Species: Mouse
PMID: 28819413
Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR
Published: 2017-01-03
Journal: TOXICOLOGY IN VITRO
Application: WB
Impact Factor: 2.87
Species: Human
PMID: 28062358
Down-Regulation of miR-129-5p Inhibits Growth and Induces Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting APC
Published: 2013-10-23
Journal: PLoS One
Application: WB
Impact Factor: 3.73
Species: Human
PMID: 24194897
Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro
Published: 2010-02-17
Journal: PHARMACEUTICAL BIOLOGY
Application: IHC
Impact Factor: 0.67
Species: Human
PMID: 20645813
Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells
Published: 2009-06-11
Journal: CANCER INVESTIGATION
Application: WB
Impact Factor: 1.98
Species: Human
PMID: 18443957
Review
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Product Promise
We promise Anti-c-Myc/MYC Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00026-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026