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Overview
| Product Name | Anti-P53 Monoclonal Antibody |
|---|---|
| SKU/Catalog Number | MA1078 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-P53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078) |
| Host | Mouse |
| Immunogen | Recombinant human wild-type p53 protein. |
| Reactivity | Human |
Assay Details
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.Images And Assay Conditions
Anti-p53 antibody (monoclonal), MA1078, Western blotting
WB: HL-60 Cell Lysate
Anti-p53 antibody (monoclonal), MA1078, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P10361 |
|---|---|
| Gene Name | Tp53 |
| Protein Name | Cellular tumor antigen p53 |
| Tissue Specificity | TP53: Ubiquitous. Isoforms are expressed in a widerange of normal tissues but in a tissue-dependent manner. Isoform2 is expressed in most normal tissues but is not detected inbrain, lung, prostate, muscle, fetal brain, spinal cord and fetalliver. Isoform 3 is expressed in most normal tissues but is notdetected in lung, spleen, testis, fetal brain, spinal cord andfetal liver. Isoform 7 is expressed in most normal tissues but isnot detected in prostate, uterus, skeletal muscle and breast.Isoform 8 is detected only in colon, bone marrow, testis, fetalbrain and intestine. Isoform 9 is expressed in most normal tissuesbut is not detected in brain, heart, lung, fetal liver, salivarygland, breast or intestine. |
| Alternative Names | Cellular tumor antigen p53;Tumor suppressor p53;Tp53;P53; |
| Subcellular Localization | Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion matrix . Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity). . |
| Molecular Weight | 43451 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2. . |
|---|---|
| Background | The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. |
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Publications
The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death
Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice
Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directedknockoutof thefull-lengthtranscriptionfactorNrf1? thatrepressesmalignantbehaviourofhumanhepatocellularcarcinoma(HepG2)cells
TALENs-directed knockout of the full-length transcription factor Nrf1? that represses malignantbehaviour of human hepatocellular carcinoma (HepG2) cells
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma
Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney
Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin
Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
MDM4 Overexpressed in Acute Myeloid Leukemia Patients with Complex Karyotype and Wild-TypeTP53
Anti-Tumor Effect of Cactus Polysaccharides on Lung Squamous Carcinoma Cells (SK-MES-1)
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
Proteomics Analysis of Normal and Senescent NG108-15 Cells: GRP78 Plays a Negative Role in Cisplatin-Induced Senescence in the NG108-15 Cell Line
Targeting of Histone Deacetylases to Reactivate Tumour Suppressor Genes and Its Therapeutic Potential in a Human Cervical Cancer Xenograft Model
c?Ski activates cancer?associated fibroblasts to regulate breast cancer cell invasion
RMP Plays Distinct Roles in the Proliferation of Hepatocellular Carcinoma Cells and Normal Hepatic Cells
SUMOylation of Mouse p53b by SUMO-1 Promotes Its Pro-Apoptotic Function in Ovarian Granulosa Cells
Establishment of a new representative model of human ovarian cancer in mice
Oxidative stress-induced 1,N6-ethenodeoxyadenosine adduct formation contributes to hepatocarcinogenesis
Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells
Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
Zhang F, Kong Ds, Zhang Zl, Lei N, Zhu Xj, Zhang Xp, Chen L, Lu Y, Zheng Sz. Apoptosis. 2013 Feb;18(2):135-49. Doi: 10.1007/S10495-012-0791-5. Tetramethylpyrazine Induces G0/G1 Cell Cycle Arrest And Stimulates Mitochondrial-Mediated And Caspase-De...
Guan Yq, Li Z, Yang A, Huang Z, Zheng Z, Zhang L, Li L, Liu Jm. Biomaterials. 2012 Sep;33(26):6162-71. Doi: 10.1016/J.Biomaterials.2012.05.037. Epub 2012 Jun 8. Cell Cycle Arrest And Apoptosis Of Ovcar-3 And Mcf-7 Cells Induced By Co-Immobilized T...
Yuan H, Jiang F, Wang R, Shen M, Chen N. Mol Med Rep. 2012 Feb;5(2):420-6. Doi: 10.3892/Mmr.2011.668. Epub 2011 Nov 7. Lentivirus-Mediated Rna Interference Of E2F-1 Suppresses Tca8113 Cell Proliferation.
Qin D, He X, Wang K, Zhao Xj, Tan W, Chen J. J Biomed Biotechnol. 2007;2007(7):89364. Doi: 10.1155/2007/89364. Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy For Detection Of Mycobacterium Tuberculosis.
Gu T, Li Cx, Feng Y, Wang Q, Li Ch, Li Cf. World J Gastroenterol. 2007 Apr 14;13(14):2113-7. Trans-Arterial Gene Therapy For Hepatocellular Carcinoma In A Rabbit Model.
Liu Xm, Yang Ff, Yuan Yf, Zhai R, Huo Lj. Plos One. 2013 May 16;8(5):E63680. Doi: 10.1371/Journal.Pone.0063680. Print 2013. Sumoylation Of Mouse P53B By Sumo-1 Promotes Its Pro-Apoptotic Function In Ovarian Granulosa Cells.
Bai J, Lei P, Zhang J, Zhao C, Liang R. Toxicology. 2013 Oct 4;312:142-8. Doi: 10.1016/J.Tox.2013.08.004. Epub 2013 Aug 21. Sulfite Exposure-Induced Hepatocyte Death Is Not Associated With Alterations In P53 Protein Expression.
Li W, Wang W, Li Y, Wang W, Wang T, Li L, Han Z, Wang S, Ma D, Wang H. Plos One. 2014 Mar 12;9(3):E90114. Doi: 10.1371/Journal.Pone.0090114. Ecollection 2014. Proteomics Analysis Of Normal And Senescent Ng108-15 Cells: Grp78 Plays A Negative Role ...
Li W, Wang W, Dong H, Li Y, Li L, Han L, Han Z, Wang S, Ma D, Wang H. Oncol Rep. 2014 Jun;31(6):2525-34. Doi: 10.3892/Or.2014.3147. Epub 2014 Apr 23. Cisplatin-Induced Senescence In Ovarian Cancer Cells Is Mediated By Grp78.
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Li Yj, Zhang Yx, Wang Py, Chi Yl, Zhang C, Ma Y, Lv Cj, Xie Sy. Oncol Rep. 2012 Jan;27(1):129-34. Doi: 10.3892/Or.2011.1466. Epub 2011 Sep 20. Regression Of A549 Lung Cancer Tumors By Anti-Mir-150 Vector.
Liang S, Peng X, Li X, Yang P, Xie L, Li Y, Du C, Zhang G. Oncotarget. 2015 Jan 20;6(2):1020-30. Silencing Of Cxcr4 Sensitizes Triple-Negative Breast Cancer Cells To Cisplatin.
Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...
Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.
Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...
Zhang S, Zhang C, Li Y, Wang P, Yue Z, Xie S. Biomed Pharmacother. 2011 Sep;65(6):436-42. Doi: 10.1016/J.Biopha.2011.04.010. Epub 2011 Jun 12. Mir-98 Regulates Cisplatin-Induced A549 Cell Death By Inhibiting Tp53 Pathway.
Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.
Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Guo H, Nan K, Hu T, Meng J, Hui W, Zhang X, Qin H, Sui C. Hepatol Res. 2010 Nov;40(11):1107-16. Doi: 10.1111/J.1872-034X.2010.00721.X. Epub 2010 Sep 28. Prognostic Significance Of Co-Expression Of Nm23 And P57 Protein In Hepatocellular Carcinoma.
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p53 antibody, tp53 antibody