Anti-P53 Tp53 Monoclonal Antibody

p53 antibody

Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: MA1078
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, ICC, WB

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Product Name

Anti-P53 Tp53 Monoclonal Antibody

View all p53 Antibodies

SKU/Catalog Number

MA1078

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P53 Tp53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

BP53-12

Isotype

Mouse IgG2a

Immunogen

Recombinant human wild-type p53 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1078 is reactive to Tp53 in Human

Applications

MA1078 is guaranteed for IHC, ICC, WB Boster Guarantee

Background of p53

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Tp53 (Source: Uniprot.org, NCBI)

Uniprot ID

P10361

Gene ID

24842

Gene Name

Tp53

Full Name

Cellular tumor antigen p53

Weight

43.451kDa

Superfamily

p53 family

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 Tp53|Trp53, p53|tumor protein p53|cellular tumor antigen p53|transformation related protein 53|tumor protein p53 (Li-Fraumeni syndrome)|tumor suppressor p53

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Tp53, check out the Tp53 Infographic

Tp53 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Tp53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1078 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Huixia Zhang,Mengda Liu,Hui Zhang et al.Detection of p53 mutation and serum monitoring alert caused by Marek‘s disease virus, 11 May 2020, PREPRINT (Version 2) available at Research Square [https://doi.org/10.21203/rs.2.23898/v2]
Species: SPF Chicken
MA1078 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN,PANCREAS AND BURSA OF FABRICIUS, DILUTION:1:200

Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
MA1078 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA

The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death

Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice

Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer

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4 Customer Q&As for Anti-P53 Tp53 Monoclonal Antibody

Question

We have seen staining in human embryonic kidney. Are there any suggestions? Is anti-P53 Monoclonal antibody supposed to stain embryonic kidney positively?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

From what I have seen in literature embryonic kidney does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2019-11-05

Question

We are currently using anti-P53 Monoclonal antibody MA1078 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

The anti-P53 Monoclonal antibody (MA1078) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-19

Question

We bought anti-P53 Monoclonal antibody for IHC on ovarian adenocarcinoma in a previous experiment. I am using human, and We are going to use the antibody for ICC next. I would like examining ovarian adenocarcinoma as well as glial cell glial tumor in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

I have checked the website and datasheets of our anti-P53 Monoclonal antibody and I see that MA1078 has been tested on human in both IHC and ICC. Thus MA1078 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-29

Question

My team were content with the WB result of your anti-P53 Monoclonal antibody. However we have seen positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?

D. Krishna

Verified customer

Asked: 2016-09-21

Answer

Based on literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2016-09-21

Size

Conjugation

Total: $315

SKU:MA1078

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MA1078
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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