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Pack Size:100μg/vial
Validated Species:Human
Clone Number:BP53-12
Application:IHC, ICC, WB
Data & Images


Product Name Anti-P53 Monoclonal Antibody
SKU/Catalog Number MA1078
Description Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-P53 Monoclonal Antibody BP53-12 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078)
Replacement Item This antibody may replace the following items: sc-100350|sc-10840|sc-10843|sc-126|sc-126-X|sc-12904-R|sc-1311-R|sc-135772|sc-135772-X|sc-135773|sc-135773-X|sc-136832|sc-137173|sc-137174|sc-137175|sc-139542|sc-139543|sc-16715-R|sc-16716-R|sc-17105-R|sc-17106-R|sc-17274-R|sc-18079-R|sc-21872-R|sc-22785|sc-244647|sc-244648|sc-249070|sc-249071|sc-376973|sc-398705|sc-48262|sc-514582|sc-514703|sc-6243-G|sc-6243-X|sc-67184|sc-71783|sc-71784|sc-71785|sc-71786|sc-71817-X|sc-7997-R|sc-7997-X|sc-81511|sc-85846|sc-85848|sc-85975|sc-98-X|sc-98384 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Human
Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Recombinant human wild-type p53 protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number BP53-12
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Tp53
Protein Name Cellular tumor antigen p53
Molecular Weight 43451 MW
Protein Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2. .
Tissue Specificity TP53: Ubiquitous. Isoforms are expressed in a widerange of normal tissues but in a tissue-dependent manner. Isoform2 is expressed in most normal tissues but is not detected inbrain, lung, prostate, muscle, fetal brain, spinal cord and fetalliver. Isoform 3 is expressed in most normal tissues but is notdetected in lung, spleen, testis, fetal brain, spinal cord andfetal liver. Isoform 7 is expressed in most normal tissues but isnot detected in prostate, uterus, skeletal muscle and breast.Isoform 8 is detected only in colon, bone marrow, testis, fetalbrain and intestine. Isoform 9 is expressed in most normal tissuesbut is not detected in brain, heart, lung, fetal liver, salivarygland, breast or intestine.
Sequence Similarities Belongs to the p53 family.
Subcellular Localization Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion matrix . Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity). .
Uniprot ID P10361
Alternative Names Cellular tumor antigen p53;Tumor suppressor p53;Tp53;P53;
Research Areas |cell biology|apoptosis|intracellular|p53 pathway|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cellular tumor antigen p53

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Anti-P53 Monoclonal Antibody Images

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Anti-P53 Monoclonal Antibody
Anti-p53 antibody (monoclonal), MA1078, Western blotting
WB: HL-60 Cell Lysate
Anti-P53 Monoclonal Antibody
Anti-p53 antibody (monoclonal), MA1078, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p53 antibody, tp53 antibody