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Product Info Summary
| SKU: | MA1078 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-P53 Tp53 Monoclonal Antibody
SKU/Catalog Number
MA1078
BM0101 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
TP53 (p53 tumor antigen) accumulates in many transformed cells and functions in the DNA-damage response by inducing cell-cycle arrest or apoptosis depending on damage severity (via transcriptional and transcription-independent apoptotic functions); the page notes TP53/p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis (contextual). Assay context: mouse monoclonal (clone BP53-12; IgG2a) raised against recombinant human wild-type p53; human-reactive; lyophilized 100 µg/vial; validated for IHC (0.5–2 µg/mL; paraffin or frozen sections), ICC (1 µg/mL), and Western blot (0.5–1 µg/mL), with a specific band detected at ~53 kDa; example WB shown across human cell lysates (A431, MCF-7, Daudi, A375, CCRF-CEM) and example IHC shown in human mammary cancer tissue. In tumor-biology panels, TP53 readouts are sometimes interpreted alongside other cancer-context markers such as CEACAM5 (CEA) and microenvironment signals like CXCL10 (IP-10) (putative); when comparing staining patterns across cohorts, a pathology review step can help standardize interpretation (workflow context).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P53 Tp53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
BP53-12
Isotype
Mouse IgG2a
Immunogen
Recombinant human wild-type p53 protein.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1078 is reactive to Tp53 in Human
Observed Molecular Weight
53 kDa
Calculated molecular weight
43.5 kDa
Background of Tp53
The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1078 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Positive Control
WB: human A431 whole cell, human MCF-7 whole cell, human Daudi whole cell, human A375 whole cell, human CCRF-CEM whole cell
IHC: human mammary cancer tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of p53 using anti-p53 antibody (MA1078).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human A375 whole cell lysates,
Lane 5: human CCRF-CEM whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p53 antigen affinity purified monoclonal antibody (Catalog # MA1078) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p53 at approximately 53 kDa. The expected band size for p53 is at 53 kDa.
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The effect of CXCR4 on protein expression and cell cycle associated proteins in TNBC cells. (A) MDA-MB-231-NC and MDA-MB-231-shCXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (B) MDA-MB-468-NC and MDA-MB-468-CXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (C) Immunohistochemical staining of xenograft tumors for p53, Bax, Bcl-2 and caspase-3 (magnification = 40×).
Index in PubMed under a CC BY license. PMID: 25544759
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IHC analysis of p53 using anti-p53 antibody (MA1078).
p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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IF analysis of p53 using anti-p53 antibody (MA1078).
p53 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-p53 Antibody (MA1078) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-P53 Tp53 Monoclonal Antibody (MA1078)
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4 Customer Q&As for Anti-P53 Tp53 Monoclonal Antibody
Question
We have seen staining in human embryonic kidney. Are there any suggestions? Is anti-P53 Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
From what I have seen in literature embryonic kidney does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-11-05
Question
We are currently using anti-P53 Monoclonal antibody MA1078 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-P53 Monoclonal antibody (MA1078) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
We bought anti-P53 Monoclonal antibody for IHC on ovarian adenocarcinoma in a previous experiment. I am using human, and We are going to use the antibody for ICC next. I would like examining ovarian adenocarcinoma as well as glial cell glial tumor in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
I have checked the website and datasheets of our anti-P53 Monoclonal antibody and I see that MA1078 has been tested on human in both IHC and ICC. Thus MA1078 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-29
Question
My team were content with the WB result of your anti-P53 Monoclonal antibody. However we have seen positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
D. Krishna
Verified customer
Asked: 2016-09-21
Answer
Based on literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2016-09-21
