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Product Info Summary
| SKU: | MA1078 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-P53 Tp53 Monoclonal Antibody
SKU/Catalog Number
MA1078
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-P53 Tp53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
BP53-12
Isotype
Mouse IgG2a
Immunogen
Recombinant human wild-type p53 protein.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
MA1078 is reactive to Tp53 in Human
Applications
MA1078 is guaranteed for IHC, ICC, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of p53 using anti-p53 antibody (MA1078).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p53 antigen affinity purified monoclonal antibody (Catalog # MA1078) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p53 at approximately 53KD. The expected band size for p53 is at 53KD.
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Figure 2. IHC analysis of p53 using anti-p53 antibody (MA1078).
p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of p53 using anti-p53 antibody (MA1078).
p53 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-p53 Antibody (MA1078) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Tp53 (Source: Uniprot.Org, NCBI)
Uniprot ID
P10361
Gene ID
24842
Gene Name
Tp53
Full Name
Cellular tumor antigen p53
Weight
43.451kDa
Superfamily
p53 family
Alternative Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 Tp53|Trp53, p53|tumor protein p53|cellular tumor antigen p53|transformation related protein 53|tumor protein p53 (Li-Fraumeni syndrome)|tumor suppressor p53
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tp53, check out the Tp53 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tp53: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-P53 Tp53 Monoclonal Antibody (MA1078)
MA1078 has been cited in 50 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huixia Zhang,Mengda Liu,Hui Zhang et al.Detection of p53 mutation and serum monitoring alert caused by Marek‘s disease virus, 11 May 2020, PREPRINT (Version 2) available at Research Square [https://doi.org/10.21203/rs.2.23898/v2]
Species: SPF Chicken
MA1078 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN,PANCREAS AND BURSA OF FABRICIUS, DILUTION:1:200
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
MA1078 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death
Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice
Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer
Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma
MDM4 Overexpressed in Acute Myeloid Leukemia Patients with Complex Karyotype and Wild-TypeTP53
Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells
The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma
Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney
Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
c?Ski activates cancer?associated fibroblasts to regulate breast cancer cell invasion
Liao S, Xiao S, Chen H, Zhang M, Chen Z, Long Y, Gao L, He J, Ge Y, Yi W, Wu M, Li G, Zhou Y. Int J Oncol. 2017 Nov;51(5):1497-1507. doi: 10.3892/ijo.2017.4137. Epub 2017 Sep 27. The receptor for activated protein kinase C promotes cell growth, in...
Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...
Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).
Zhang F, Kong Ds, Zhang Zl, Lei N, Zhu Xj, Zhang Xp, Chen L, Lu Y, Zheng Sz. Apoptosis. 2013 Feb;18(2):135-49. Doi: 10.1007/S10495-012-0791-5. Tetramethylpyrazine Induces G0/G1 Cell Cycle Arrest And Stimulates Mitochondrial-Mediated And Caspase-De...
Guan Yq, Li Z, Yang A, Huang Z, Zheng Z, Zhang L, Li L, Liu Jm. Biomaterials. 2012 Sep;33(26):6162-71. Doi: 10.1016/J.Biomaterials.2012.05.037. Epub 2012 Jun 8. Cell Cycle Arrest And Apoptosis Of Ovcar-3 And Mcf-7 Cells Induced By Co-Immobilized T...
Yuan H, Jiang F, Wang R, Shen M, Chen N. Mol Med Rep. 2012 Feb;5(2):420-6. Doi: 10.3892/Mmr.2011.668. Epub 2011 Nov 7. Lentivirus-Mediated Rna Interference Of E2F-1 Suppresses Tca8113 Cell Proliferation.
Qin D, He X, Wang K, Zhao Xj, Tan W, Chen J. J Biomed Biotechnol. 2007;2007(7):89364. Doi: 10.1155/2007/89364. Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy For Detection Of Mycobacterium Tuberculosis.
Gu T, Li Cx, Feng Y, Wang Q, Li Ch, Li Cf. World J Gastroenterol. 2007 Apr 14;13(14):2113-7. Trans-Arterial Gene Therapy For Hepatocellular Carcinoma In A Rabbit Model.
Liu Xm, Yang Ff, Yuan Yf, Zhai R, Huo Lj. Plos One. 2013 May 16;8(5):E63680. Doi: 10.1371/Journal.Pone.0063680. Print 2013. Sumoylation Of Mouse P53B By Sumo-1 Promotes Its Pro-Apoptotic Function In Ovarian Granulosa Cells.
Bai J, Lei P, Zhang J, Zhao C, Liang R. Toxicology. 2013 Oct 4;312:142-8. Doi: 10.1016/J.Tox.2013.08.004. Epub 2013 Aug 21. Sulfite Exposure-Induced Hepatocyte Death Is Not Associated With Alterations In P53 Protein Expression.
Li W, Wang W, Li Y, Wang W, Wang T, Li L, Han Z, Wang S, Ma D, Wang H. Plos One. 2014 Mar 12;9(3):E90114. Doi: 10.1371/Journal.Pone.0090114. Ecollection 2014. Proteomics Analysis Of Normal And Senescent Ng108-15 Cells: Grp78 Plays A Negative Role ...
Li W, Wang W, Dong H, Li Y, Li L, Han L, Han Z, Wang S, Ma D, Wang H. Oncol Rep. 2014 Jun;31(6):2525-34. Doi: 10.3892/Or.2014.3147. Epub 2014 Apr 23. Cisplatin-Induced Senescence In Ovarian Cancer Cells Is Mediated By Grp78.
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Li Yj, Zhang Yx, Wang Py, Chi Yl, Zhang C, Ma Y, Lv Cj, Xie Sy. Oncol Rep. 2012 Jan;27(1):129-34. Doi: 10.3892/Or.2011.1466. Epub 2011 Sep 20. Regression Of A549 Lung Cancer Tumors By Anti-Mir-150 Vector.
Liang S, Peng X, Li X, Yang P, Xie L, Li Y, Du C, Zhang G. Oncotarget. 2015 Jan 20;6(2):1020-30. Silencing Of Cxcr4 Sensitizes Triple-Negative Breast Cancer Cells To Cisplatin.
Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...
Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.
Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...
Zhang S, Zhang C, Li Y, Wang P, Yue Z, Xie S. Biomed Pharmacother. 2011 Sep;65(6):436-42. Doi: 10.1016/J.Biopha.2011.04.010. Epub 2011 Jun 12. Mir-98 Regulates Cisplatin-Induced A549 Cell Death By Inhibiting Tp53 Pathway.
Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.
Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Guo H, Nan K, Hu T, Meng J, Hui W, Zhang X, Qin H, Sui C. Hepatol Res. 2010 Nov;40(11):1107-16. Doi: 10.1111/J.1872-034X.2010.00721.X. Epub 2010 Sep 28. Prognostic Significance Of Co-Expression Of Nm23 And P57 Protein In Hepatocellular Carcinoma.
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
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Customer Q&As
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4 Customer Q&As for Anti-P53 Tp53 Monoclonal Antibody
Question
We have seen staining in human embryonic kidney. Are there any suggestions? Is anti-P53 Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
From what I have seen in literature embryonic kidney does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-11-05
Question
We are currently using anti-P53 Monoclonal antibody MA1078 for human tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-P53 Monoclonal antibody (MA1078) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
We bought anti-P53 Monoclonal antibody for IHC on ovarian adenocarcinoma in a previous experiment. I am using human, and We are going to use the antibody for ICC next. I would like examining ovarian adenocarcinoma as well as glial cell glial tumor in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
I have checked the website and datasheets of our anti-P53 Monoclonal antibody and I see that MA1078 has been tested on human in both IHC and ICC. Thus MA1078 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-29
Question
My team were content with the WB result of your anti-P53 Monoclonal antibody. However we have seen positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
D. Krishna
Verified customer
Asked: 2016-09-21
Answer
Based on literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2016-09-21
