Anti-Apc2 ANAPC2 Antibody

Product Name

Anti-Apc2 ANAPC2 Antibody

View all ANAPC2 Antibodies

SKU/Catalog Number

A06153

Size

100uL

Form

Liquid (sterile filtered)

Description

ANAPC2 (APC2) is a core subunit of the anaphase-promoting complex/cyclosome (APC/C), a cell-cycle regulated E3 ubiquitin ligase that drives mitotic progression and G1 control by promoting ubiquitination and degradation of key regulators (e.g., securin, cyclins) in curated APC/C pathways. Assay context: human-reactive antibody tested for IHC and Western blot, supporting proliferation-state mapping in tumors or regenerating tissues; often interpreted alongside genomic-stress regulators such as TP53 (putative co-panel).

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Apc2 ANAPC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06153)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH.

Reactive Species

A06153 is reactive to ANAPC2 in Human

Observed Molecular Weight

39 kDa

Calculated molecular weight

93.8 kDa

Background of ANAPC2

APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06153 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
WB: 1:500 - 1:1,000
This antibody reacts with human APC2 by western blot and immunoprecipitation.  The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others).  Coimmunoprecipitation of related proteins (APC11) does occur.  A 93.8 kDa band corresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 can be used as a positive control.  Researchers should determine optimal titers for other applications.

Validation Images & Assay Conditions

See full target information ANAPC2

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