Anti-Cyclophilin B/PPIB Antibody Picoband™

Cyclophilin B antibody

Boster Bio Anti-Cyclophilin B/PPIB Antibody Picoband™ catalog # A03229. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Independently reviewed in 1 review(s).

Product Info Summary

SKU: A03229
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Cyclophilin B/PPIB Antibody Picoband™

View all Cyclophilin B Antibodies

SKU/Catalog Number

A03229

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclophilin B/PPIB Antibody Picoband™ catalog # A03229. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclophilin B/PPIB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03229)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Cyclophilin B recombinant protein (Position: K158-E216).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03229 is reactive to PPIB in Human, Mouse, Rat

Applications

A03229 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

23.743kDa

Background of Cyclophilin B

Peptidyl-prolyl cis-trans isomerase B, also known as CYPB, is an enzyme that in humans is encoded by the PPIB gene. This gene is mapped to 15q22.31. The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For PPIB (Source: Uniprot.org, NCBI)

Gene Name

PPIB

Full Name

Peptidyl-prolyl cis-trans isomerase B

Weight

23.743kDa

Superfamily

cyclophilin-type PPIase family

Alternative Names

Cyclophilin B; cyclophilin-like protein; CYPB; CYPBEC 5.2.1.8; CYP-S1; MGC2224; OI9MGC14109; peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase B; PPIB; Rotamase B; S-cyclophilin; SCYLP PPIB B, CYP-S1, CYPB, HEL-S-39, OI9, SCYLP peptidylprolyl isomerase B peptidyl-prolyl cis-trans isomerase B|PPIase B|S-cyclophilin|cyclophilin-like protein|epididymis secretory protein Li 39|peptidylprolyl isomerase B (cyclophilin B)|rotamase B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPIB, check out the PPIB Infographic

PPIB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPIB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Reviews For Anti-Cyclophilin B/PPIB Antibody Picoband™

0

Immunohistochemistry for Anti-Cyclophilin B/PPIB Antibody

Excellent

Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

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Order DetailsPrice
A03229

100μg

$370
A03229-10ug

10μg sample (liquid)

$99
A03229-Biotin

100 μg Biotin conjugated

$570
A03229-Cy3

100 μg Cy3 conjugated

$570
A03229-Dylight488

100 μg Dylight488 conjugated

$570
A03229-Dylight550

100 μg Dylight550 conjugated

$570
A03229-Dylight594

100 μg Dylight594 conjugated

$570
A03229-FITC

100 μg FITC conjugated

$570
A03229-HRP

100 μg HRP conjugated

$570
A03229-APC

100 μg APC conjugated

$670
A03229-PE

100 μg PE conjugated

$670
A03229-carrier-free

Carrier Free

$370

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A03229
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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