Anti-C1QB Antibody

C1QB antibody

Boster Bio Anti-C1QB Antibody (Catalog # A04233). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A04233
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-C1QB Antibody

View all C1QB Antibodies

SKU/Catalog Number

A04233

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-C1QB Antibody (Catalog # A04233). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C1QB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04233)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human C1QB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04233 is reactive to C1QB in Human

Applications

A04233 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

26.722kDa

Background of C1QB

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

Reid K.B.M.; Biochem. J. 231:729-735(1985).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For C1QB (Source: Uniprot.org, NCBI)

Gene Name

C1QB

Full Name

Complement C1q subcomponent subunit B

Weight

26.722kDa

Alternative Names

B chain; complement C1q subcomponent subunit B; complement component 1, q subcomponent, B chain; complement subcomponent C1q chain B; q subcomponent, beta polypeptide C1QB complement C1q B chain complement C1q subcomponent subunit B|complement C1q chain B|complement component 1, q subcomponent, B chain|complement component 1, q subcomponent, beta polypeptide|complement component C1q, B chain|complement subcomponent C1q chain B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on C1QB, check out the C1QB Infographic

C1QB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for C1QB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-C1QB Antibody

Question

See attached the WB image, lot number and protocol we used for liver using anti-C1QB antibody A04233. Please let me know if you require anything else.

A. Taylor

Verified customer

Asked: 2020-04-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-15

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-C1QB antibody A04233. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-09

Question

Is this A04233 anti-C1QB antibody reactive to the isotypes of C1QB?

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

The immunogen of A04233 anti-C1QB antibody is Synthesized peptide derived from internal of human C1QB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-07

Question

My lab would like to test anti-C1QB antibody A04233 on human liver for research purposes, then I may be interested in using anti-C1QB antibody A04233 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The products we sell, including anti-C1QB antibody A04233, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-03

Question

We are currently using anti-C1QB antibody A04233 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The anti-C1QB antibody (A04233) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-08

Question

Will anti-C1QB antibody A04233 work on horse IF with brain?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

Our lab technicians have not tested anti-C1QB antibody A04233 on horse. You can run a BLAST between horse and the immunogen sequence of anti-C1QB antibody A04233 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-29

Question

Is a blocking peptide available for product anti-C1QB antibody (A04233)?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

We do provide the blocking peptide for product anti-C1QB antibody (A04233). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-24

Question

Would A04233 anti-C1QB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

As indicated on the product datasheet, A04233 anti-C1QB antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-10

Question

My question regarding product A04233, anti-C1QB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Li

Verified customer

Asked: 2018-03-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04233 anti-C1QB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-03-19

Question

Do you have a BSA free version of anti-C1QB antibody A04233 available?

P. Kulkarni

Verified customer

Asked: 2016-08-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C1QB antibody A04233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-08-01

Question

I was wanting to use your anti-C1QB antibody for IF for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

R. Brown

Verified customer

Asked: 2015-07-06

Answer

As indicated on the product datasheet, A04233 anti-C1QB antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-06

Question

Does anti-C1QB antibody A04233 work for IF with liver?

P. Jackson

Verified customer

Asked: 2014-09-09

Answer

According to the expression profile of liver, C1QB is highly expressed in liver. So, it is likely that anti-C1QB antibody A04233 will work for IF with liver.

Boster Scientific Support

Answered: 2014-09-09

Question

I see that the anti-C1QB antibody A04233 works with IF, what is the protocol used to produce the result images on the product page?

C. Mitchell

Verified customer

Asked: 2013-04-19

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-19

Order DetailsPrice
A04233-100uL

100uL

$360

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A04233
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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