Anti-Calpain 1/CAPN1 Antibody

Calpain 1 antibody

Boster Bio Anti-Calpain 1/CAPN1 Antibody catalog # PA1364. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1364
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Calpain 1/CAPN1 Antibody

View all Calpain 1 Antibodies

SKU/Catalog Number

PA1364

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calpain 1/CAPN1 Antibody catalog # PA1364. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calpain 1/CAPN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1364)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1 (312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1364 is reactive to CAPN1 in Human, Mouse, Rat

Applications

PA1364 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of Calpain 1

Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS; 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CAPN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07384

Gene ID

823

Gene Name

CAPN1

Full Name

Calpain-1 catalytic subunit

Weight

81.89kDa

Superfamily

peptidase C2 family

Alternative Names

Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; CANP 1; CANP; CANPL1EC 3.4.22.52; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; EC 3.4.22; micromolar-calpain; muCANPCANP1; muCL CAPN1 CANP, CANP1, CANPL1, SPG76, muCANP, muCL calpain 1 calpain-1 catalytic subunit|CANP 1|calcium-activated neutral proteinase 1|calpain 1, (mu/I) large subunit|calpain mu-type|calpain, large polypeptide L1|calpain-1 large subunit|cell proliferation-inducing gene 30 protein|cell proliferation-inducing protein 30|micromolar-calpain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CAPN1, check out the CAPN1 Infographic

CAPN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CAPN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1364 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Mo Xg, Chen Qw, Li Xs, Zheng Mm, Ke Dz, Deng W, Li Gq, Jiang J, Wu Zq, Wang L, Wang P, Yang Y, Cao Gy. Microvasc Res. 2011 Mar;81(2):160-8. Doi: 10.1016/J.Mvr.2010.12.004. Epub 2010 Dec 24. Suppression Of Nhe1 By Small Interfering Rna Inhibits Hif...

Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.

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17 Customer Q&As for Anti-Calpain 1/CAPN1 Antibody

Question

I was wanting to use your anti-Calpain 1/CAPN1 antibody for IHC for mouse pancreas placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pancreas placenta identification?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

It shows on the product datasheet, PA1364 anti-Calpain 1/CAPN1 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse pancreas placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-30

Question

See below the WB image, lot number and protocol we used for pancreas placenta using anti-Calpain 1/CAPN1 antibody PA1364. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-23

Question

We are currently using anti-Calpain 1/CAPN1 antibody PA1364 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

The anti-Calpain 1/CAPN1 antibody (PA1364) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-02

Question

Will anti-Calpain 1/CAPN1 antibody PA1364 work for IHC with pancreas placenta?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

According to the expression profile of pancreas placenta, CAPN1 is highly expressed in pancreas placenta. So, it is likely that anti-Calpain 1/CAPN1 antibody PA1364 will work for IHC with pancreas placenta.

Boster Scientific Support

Answered: 2020-02-20

Question

My boss were well pleased with the WB result of your anti-Calpain 1/CAPN1 antibody. However we have been able to see positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is CAPN1 supposed to be expressed?

L. Li

Verified customer

Asked: 2020-01-15

Answer

Based on literature, kidney does express CAPN1. Generally CAPN1 expresses in cytoplasm. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-01-15

Question

Do you have a BSA free version of anti-Calpain 1/CAPN1 antibody PA1364 available?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Calpain 1/CAPN1 antibody PA1364 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-10

Question

Can you help my question with product PA1364, anti-Calpain 1/CAPN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1364 anti-Calpain 1/CAPN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-17

Question

Is this PA1364 anti-Calpain 1/CAPN1 antibody reactive to the isotypes of CAPN1?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

The immunogen of PA1364 anti-Calpain 1/CAPN1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-02

Question

We ordered your anti-Calpain 1/CAPN1 antibody for IHC on kidney a few months ago. I am using human, and We are going to use the antibody for ICC next. I am interested in examining kidney as well as erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

I took a look at the website and datasheets of our anti-Calpain 1/CAPN1 antibody and it appears that PA1364 has been tested on human in both IHC and ICC. Thus PA1364 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-19

Question

Does anti-Calpain 1/CAPN1 antibody PA1364 work on feline ICC with erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

Our lab technicians have not validated anti-Calpain 1/CAPN1 antibody PA1364 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Calpain 1/CAPN1 antibody PA1364 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline erythroleukemia in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-24

Question

Our lab want to know about using your anti-Calpain 1/CAPN1 antibody for autosomal recessive (spg76) studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

Thank you for your inquiry. This PA1364 anti-Calpain 1/CAPN1 antibody is tested on mouse ovary tissue, spleen tissue, lung tissue, kidney tissue, brain tissue, thymus tissue, small intestine tissue, rat thymus tissue, heart tissue, hela whole cell lysates, human hela, cardiac muscle tissue, testis tissue, colo320 whole cell lysates. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-06-28

Question

Would PA1364 anti-Calpain 1/CAPN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

As indicated on the product datasheet, PA1364 anti-Calpain 1/CAPN1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-29

Question

We have been able to see staining in rat pancreas placenta. Do you have any suggestions? Is anti-Calpain 1/CAPN1 antibody supposed to stain pancreas placenta positively?

R. Williams

Verified customer

Asked: 2018-09-17

Answer

From literature pancreas placenta does express CAPN1. From Uniprot.org, CAPN1 is expressed in esophagus mucosa, kidney, pancreas placenta, erythroleukemia, liver, among other tissues. Regarding which tissues have CAPN1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pancreas, and Placenta, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-09-17

Question

I see that the anti-Calpain 1/CAPN1 antibody PA1364 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-08-09

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-09

Question

Is a blocking peptide available for product anti-Calpain 1/CAPN1 antibody (PA1364)?

T. Krishna

Verified customer

Asked: 2018-03-13

Answer

We do provide the blocking peptide for product anti-Calpain 1/CAPN1 antibody (PA1364). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-03-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas placenta using anti-Calpain 1/CAPN1 antibody PA1364. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-16

Question

I was wanting to use to test anti-Calpain 1/CAPN1 antibody PA1364 on mouse pancreas placenta for research purposes, then I may be interested in using anti-Calpain 1/CAPN1 antibody PA1364 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Kulkarni

Verified customer

Asked: 2013-10-29

Answer

The products we sell, including anti-Calpain 1/CAPN1 antibody PA1364, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-10-29

Size

Total: $315

SKU:PA1364

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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