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Product Info Summary
| SKU: | M03492-1 |
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| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4]
SKU/Catalog Number
M03492-1
Size
100 µl
Description
Boster Bio CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4). Catalog# M03492-1. Tested in FC, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03492-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human CAPN2 (NP_001739) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03492-1 is reactive to CAPN2 in Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
79.995kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03492-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either CAPN2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAPN2 antibody (M03492-1)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and CAPN2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CAPN2 monoclonal antibody M03492-1
Protein Target Info & Infographic
Gene/Protein Information For CAPN2 (Source: Uniprot.org, NCBI)
Gene Name
CAPN2
Full Name
Calpain-2 catalytic subunit
Weight
79.995kDa
Superfamily
peptidase C2 family
Alternative Names
Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; CANPL2CANP2; CANPml; EC 3.4.22; EC 3.4.22.53; FLJ39928; M-calpain; mCANP; millimolar-calpain CAPN2 CANP2, CANPL2, CANPml, mCANP calpain 2 calpain-2 catalytic subunit|CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, (m/II) large subunit|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain, large polypeptide L2|millimolar-calpain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAPN2, check out the CAPN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAPN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4] (M03492-1)
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