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Product Info Summary
| SKU: | M03492-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4]
SKU/Catalog Number
M03492-1
Size
100 µl
Description
Boster Bio CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4). Catalog# M03492-1. Tested in FC, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03492-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1F4
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human CAPN2 (NP_001739) produced in HEK293T cell.
Reactive Species
M03492-1 is reactive to CAPN2 in Human, Monkey, Mouse, Rat
Calculated molecular weight
80.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03492-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either CAPN2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAPN2 antibody (M03492-1)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and CAPN2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-CAPN2 monoclonal antibody M03492-1
Specific Publications For Anti-Calpain 2 (CAPN2) Mouse Monoclonal Antibody [Clone ID: OTI1F4] (M03492-1)
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