Click image to see more details
Product Info Summary
| SKU: | P00577-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00577-1
BM3949 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody catalog # P00577-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00577-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Creb (S133)
Reactive Species
P00577-1 is reactive to CREB1 in Human, Mouse, Rat
Observed Molecular Weight
44 kDa
Calculated molecular weight
36.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00577-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:40
Positive Control
IHC: human gastric carcinoma tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Phospho-Creb(Ser133) expression in HeLa cell lysates treated with Calyculin A.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody.
Specific Publications For Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody (P00577-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Phospho-Creb (S133) CREB1 Rabbit Monoclonal Antibody
Question
Our team were satisfied with the WB result of your anti-Phospho-Creb (S133) Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is CREB1 supposed to be expressed?
B. Taylor
Verified customer
Asked: 2018-03-23
Answer
Based on literature, cervix carcinoma erythroleukemia does express CREB1. Generally CREB1 expresses in nucleus. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2018-03-23
Question
We have seen staining in rat testis. Any tips? Is anti-Phospho-Creb (S133) Rabbit Monoclonal antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
According to literature testis does express CREB1. According to Uniprot.org, CREB1 is expressed in lung, testis, eye, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2017-12-01
Question
We bought anti-Phospho-Creb (S133) Rabbit Monoclonal antibody for Flow Cytometry on cervix carcinoma in the past. I am using mouse, and We intend to use the antibody for ICC next. My lab would like examining cervix carcinoma as well as embryonic kidney in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
S. Jha
Verified customer
Asked: 2015-06-16
Answer
I viewed the website and datasheets of our anti-Phospho-Creb (S133) Rabbit Monoclonal antibody and I see that P00577-1 has been tested on mouse in both Flow Cytometry and ICC. Thus P00577-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-06-16
Question
We are currently using anti-Phospho-Creb (S133) Rabbit Monoclonal antibody P00577-1 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
V. Thomas
Verified customer
Asked: 2014-10-22
Answer
The anti-Phospho-Creb (S133) Rabbit Monoclonal antibody (P00577-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-22
