Anti-BDNF Antibody

Boster Bio Anti-BDNF Antibody catalog # PA1014. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 17 publication(s).

Product Info Summary

SKU: PA1014
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-BDNF Antibody

See all Bdnf products

SKU/Catalog Number

PA1014

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BDNF Antibody catalog # PA1014. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BDNF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1014)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human BDNF (129-148aa HSDPARRGELSVCDSISEWV), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1014 is reactive to BDNF in Human, Mouse, Rat

Applications

PA1014 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For BDNF (Source: Uniprot.Org, NCBI)

Uniprot ID

P23560

Gene ID

627

Gene Name

BDNF

Full Name

Brain-derived neurotrophic factor

Weight

27.818kDa

Superfamily

NGF-beta family

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BDNF, check out the BDNF Infographic

BDNF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1014 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xue LL,Du RL,Hu Y,Xiong LL,Su ZY,Ma Z,Tan YX,Liu J,Hu Q,Zhu ZQ,Wang TH,Liu XZ.BDNF promotes neuronal survival after neonatal hypoxic-ischemic encephalopathy by up-regulating Stx1b and suppressing VDAC1.Brain Res Bull.2021 May 28:S0361-9230(21)00144-1.doi:10.1016/j.brainresbull.2021.05.013.Epub ahead of print.PMID:34058286.
Species: Rat
PA1014 usage in article: APP:WB, SAMPLE:CORTEX AND HIPPOCAMPUS, DILUTION:1:100

Feng,S.,Wang,S.,Sun,S.,Su,H.,& Zhang,L.(2021).Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.Molecular Medicine Reports,23,294.h
Species: Rat
PA1014 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:200

Huangqin flavonoid extraction for spinal cord injury in a rat model

Serotonin regulates brain-derived neurotrophic factor expression in select brain regions during acute psychological stress

Beneficial effect of fluoxetine treatment aganist psychological stress is mediated by increasing BDNF expression in selected brain areas

Brain-derived neurotrophic factor is up-regulated in severe acute cauda equina syndrome dog model

Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation

Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles

Panax notoginsenoside Rb1 ameliorates Alzheimer?s disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression

Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus

Effects of Polysaccharide of Gastrodia Elata Blume and Electro-Acupuncture on Expressions of Brain-Derived Neurotrophic Factor and Stem Cell Factor Protein in Caudate Putamen of Focal Cerebral Ischemia Rats

Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion

Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Effects of senescence on the expression of BDNF and TrkB receptor in the lateral geniculate nucleus of cats

Li R, Xia W, Zhang Z, Wu K. Plos One. 2011;6(6):E21663. Doi: 10.1371/Journal.Pone.0021663. Epub 2011 Jun 27. S100B Protein, Brain-Derived Neurotrophic Factor, And Glial Cell Line-Derived Neurotrophic Factor In Human Milk.

Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.

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9 Customer Q&As for Anti-BDNF Antibody

Question

Does PA1014 react to mature, precursor, or both forms of BDNF? Keywords: pro-BDNF, m-BDNF, isoform

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

PA1014 reacts to pre-cursor BDNF.

Boster Scientific Support

Answered: 2020-04-30

Question

For the BDNF antibody associated with ECL detection, what type of gel and gradient do you use?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

We use SDS-PAGE gel with 5% concentrated gel and 12% resolving gel.

Boster Scientific Support

Answered: 2020-04-16

Question

Will anti-BDNF antibody PA1014 work on primate WB with leukocyte?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

Our lab technicians have not tested anti-BDNF antibody PA1014 on primate. You can run a BLAST between primate and the immunogen sequence of anti-BDNF antibody PA1014 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukocyte in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-07

Question

Please see the WB image, lot number and protocol we used for serum using anti-BDNF antibody PA1014. Please let me know if you require anything else.

J. Jha

Verified customer

Asked: 2018-06-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-15

Question

My question regards to test anti-BDNF antibody PA1014 on rat serum for research purposes, then I may be interested in using anti-BDNF antibody PA1014 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-26

Answer

The products we sell, including anti-BDNF antibody PA1014, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-26

Question

I was wanting to use your anti-BDNF antibody for WB for rat serum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat serum identification?

K. Jackson

Verified customer

Asked: 2016-11-10

Answer

As indicated on the product datasheet, PA1014 anti-BDNF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat serum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-11-10

Question

Is there a BSA free version of anti-BDNF antibody PA1014 available?

P. Jha

Verified customer

Asked: 2016-01-26

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BDNF antibody PA1014 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-01-26

Question

Would PA1014 anti-BDNF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Jackson

Verified customer

Asked: 2015-10-22

Answer

It shows on the product datasheet, PA1014 anti-BDNF antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-10-22

Question

Is a blocking peptide available for product anti-BDNF antibody (PA1014)?

J. Kulkarni

Verified customer

Asked: 2013-12-31

Answer

We do provide the blocking peptide for product anti-BDNF antibody (PA1014). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-12-31

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PA1014
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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