Anti-Calpain 2/CAPN2 Antibody Picoband™

Calpain 2 antibody

Boster Bio Anti-Calpain 2/CAPN2 Antibody Picoband™ catalog # A03492. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A03492
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Calpain 2/CAPN2 Antibody Picoband™

View all Calpain 2 Antibodies

SKU/Catalog Number

A03492

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calpain 2/CAPN2 Antibody Picoband™ catalog # A03492. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calpain 2/CAPN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03492)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03492 is reactive to CAPN2 in Human, Mouse, Rat

Applications

A03492 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

80 kDa

Calculated molecular weight

79.995kDa

Background of Calpain 2

Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat,
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CAPN2 (Source: Uniprot.org, NCBI)

Gene Name

CAPN2

Full Name

Calpain-2 catalytic subunit

Weight

79.995kDa

Superfamily

peptidase C2 family

Alternative Names

Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; CANPL2CANP2; CANPml; EC 3.4.22; EC 3.4.22.53; FLJ39928; M-calpain; mCANP; millimolar-calpain CAPN2 CANP2, CANPL2, CANPml, mCANP calpain 2 calpain-2 catalytic subunit|CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, (m/II) large subunit|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain, large polypeptide L2|millimolar-calpain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CAPN2, check out the CAPN2 Infographic

CAPN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CAPN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A03492 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway

Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.

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Order DetailsPrice
A03492

100μg

$370
A03492-10ug

10μg sample (liquid)

$99
A03492-Biotin

100 μg Biotin conjugated

$570
A03492-Cy3

100 μg Cy3 conjugated

$570
A03492-Dylight488

100 μg Dylight488 conjugated

$570
A03492-Dylight550

100 μg Dylight550 conjugated

$570
A03492-Dylight594

100 μg Dylight594 conjugated

$570
A03492-FITC

100 μg FITC conjugated

$570
A03492-HRP

100 μg HRP conjugated

$570
A03492-APC

100 μg APC conjugated

$670
A03492-PE

100 μg PE conjugated

$670
A03492-carrier-free

Carrier Free

$370

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