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Product Info Summary
| SKU: | PA1815 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-GRP78 BiP/HSPA5 Antibody Picoband®
SKU/Catalog Number
PA1815
BA2042 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRP78 BiP/HSPA5 Antibody catalog # PA1815. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRP78 BiP/HSPA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1815)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1815 is reactive to HSPA5 in Human, Mouse, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
72.3 kDa
Background of HSPA5
HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1815 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human U251 whole cell, human HepG2 whole cell, human K562 whole cell, human A431 whole cell, human Hela whole cell, human SiHa whole cell, human A549 whole cell, human 293T whole cell, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue
IHC: Rat Intestine Tissue, Rat Cerebellum Tissue, Human Lung Cancer Tissue
IHC-F: Rat Intestine Tissue
ICC: HELA Cell
Validation Images & Assay Conditions
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Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A431 whole cell lysates.
Lane 5: human Hela whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (PA1815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 78 kDa. The expected band size for HSPA5 is at 72 kDa.
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Anti-GRP78 BiP antibody, PA1815, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-GRP78 BiP antibody, PA1815, IHC(P)
IHC(P): Rat Cerebellum Tissue
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Anti-GRP78 BiP antibody, PA1815, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-GRP78 BiP antibody, PA1815, ICC
ICC: HELA Cell
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Anti-GRP78 BiP antibody, PA1815, IHC(F)
IHC(F): Rat Intestine Tissue
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Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (PA1815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 78 kDa. The expected band size for HSPA5 is at 72 kDa.
Click image to see more details
Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-6: Different segments of the porcine large intestine and small intestine lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified monoclonal antibody (Catalog # PA1815) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for HSPA5 at approximately 70-78 kDa. The expected band size for HSPA5 is at 73 kDa.
Specific Publications For Anti-GRP78 BiP/HSPA5 Antibody Picoband® (PA1815)
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Customer Reviews
Have you used Anti-GRP78 BiP/HSPA5 Antibody Picoband®?
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1 Reviews For Anti-GRP78 BiP/HSPA5 Antibody Picoband®
The target band of this antibody is clear and at the correct position, and it shows strong affinity for porcine GRP78 protein.
Excellent
| SKU | PA1815 |
|---|---|
| Application | Western Blot |
| Sample | Porcine intestinal tissue |
| Sample Processing Description | Intestinal tissues from different segments of pigs infected with swine fever. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Anti-GRP78 BiP/HSPA5 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | When cells experience stress such as hypoxia, nutrient deprivation, or calcium imbalance, large amounts of unfolded proteins accumulate in the endoplasmic reticulum. GRP78 senses this stress and undergoes dissociation upon upregulation, activating a series of signaling pathways to reduce new protein synthesis and increase the production of molecular chaperones (including GRP78 itself) to relieve the stress. The aim of the experiment is to determine the expression levels of GRP78 in different intestinal segments of pigs infected with swine fever. |
Lu Jin, Zhejiang A&F University
Verified customer
Submitted 2025-12-02
Customer Q&As
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17 Customer Q&As for Anti-GRP78 BiP/HSPA5 Antibody Picoband®
Question
Will PA1815 anti-GRP78 BiP/HSPA5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
You can see on the product datasheet, PA1815 anti-GRP78 BiP/HSPA5 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-01
Question
I was wanting to use your anti-GRP78 BiP/HSPA5 antibody for ICC for rat articular cartilage on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat articular cartilage identification?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
It shows on the product datasheet, PA1815 anti-GRP78 BiP/HSPA5 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat articular cartilage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-24
Question
Please see the WB image, lot number and protocol we used for articular cartilage using anti-GRP78 BiP/HSPA5 antibody PA1815. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
I see that the anti-GRP78 BiP/HSPA5 antibody PA1815 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-08
Question
Would anti-GRP78 BiP/HSPA5 antibody PA1815 work for ICC with articular cartilage?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
According to the expression profile of articular cartilage, HSPA5 is highly expressed in articular cartilage. So, it is likely that anti-GRP78 BiP/HSPA5 antibody PA1815 will work for ICC with articular cartilage.
Boster Scientific Support
Answered: 2019-12-13
Question
I would like using your anti-GRP78 BiP/HSPA5 antibody for cellular response to glucose starvation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thanks for your inquiry. This PA1815 anti-GRP78 BiP/HSPA5 antibody is validated on rat cerebellum tissue, testis tissue, tissue lysate, a549 cell lysate, hela cell lysate, intestine tissue, lung cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-03
Question
Can you help my question with product PA1815, anti-GRP78 BiP/HSPA5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1815 anti-GRP78 BiP/HSPA5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
Our team were happy with the WB result of your anti-GRP78 BiP/HSPA5 antibody. However we have been able to see positive staining in mammary carcinoma endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is HSPA5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
From literature, mammary carcinoma does express HSPA5. Generally HSPA5 expresses in endoplasmic reticulum lumen. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-30
Question
Is this PA1815 anti-GRP78 BiP/HSPA5 antibody reactive to the isotypes of HSPA5?
J. Jackson
Verified customer
Asked: 2019-08-30
Answer
The immunogen of PA1815 anti-GRP78 BiP/HSPA5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP(603-617aa EWLESHQDADIEDFK), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
We purchased anti-GRP78 BiP/HSPA5 antibody for WB on cajal-retzius cell fetal brain cortex last year. I am using rat, and We are going to use the antibody for IHC next. I am interested in examining cajal-retzius cell fetal brain cortex as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
I have checked the website and datasheets of our anti-GRP78 BiP/HSPA5 antibody and it appears that PA1815 has been validated on rat in both WB and IHC. Thus PA1815 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-GRP78 BiP/HSPA5 antibody (PA1815)?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
We do provide the blocking peptide for product anti-GRP78 BiP/HSPA5 antibody (PA1815). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-08
Question
We are currently using anti-GRP78 BiP/HSPA5 antibody PA1815 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
The anti-GRP78 BiP/HSPA5 antibody (PA1815) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-03
Question
We have been able to see staining in mouse brain. Any tips? Is anti-GRP78 BiP/HSPA5 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
From what I have seen in literature brain does express HSPA5. From what I have seen in Uniprot.org, HSPA5 is expressed in vena cava, cervix carcinoma, fibroblast, muscle, articular cartilage, mammary carcinoma, colon carcinoma, brain, cajal-retzius cell fetal brain cortex, melanoma, liver, among other tissues. Regarding which tissues have HSPA5 expression, here are a few articles citing expression in various tissues:
Articular cartilage, Pubmed ID: 11160188
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1550958
Cervix carcinoma, Pubmed ID: 20068231
Colon carcinoma, Pubmed ID: 9150948, 24129315
Fibroblast, Pubmed ID: 15164053
Liver, Pubmed ID: 24275569
Mammary carcinoma, Pubmed ID: 9150946
Melanoma, Pubmed ID: 12643545, 17081065
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-04-26
Question
Would anti-GRP78 BiP/HSPA5 antibody PA1815 work on pig ICC with articular cartilage?
A. Jackson
Verified customer
Asked: 2017-11-10
Answer
Our lab technicians have not validated anti-GRP78 BiP/HSPA5 antibody PA1815 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GRP78 BiP/HSPA5 antibody PA1815 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig articular cartilage in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-10
Question
My question regards to test anti-GRP78 BiP/HSPA5 antibody PA1815 on rat articular cartilage for research purposes, then I may be interested in using anti-GRP78 BiP/HSPA5 antibody PA1815 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jones
Verified customer
Asked: 2013-12-23
Answer
The products we sell, including anti-GRP78 BiP/HSPA5 antibody PA1815, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-12-23
Question
Do you have a BSA free version of anti-GRP78 BiP/HSPA5 antibody PA1815 available?
R. Thomas
Verified customer
Asked: 2013-11-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GRP78 BiP/HSPA5 antibody PA1815 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for articular cartilage using anti-GRP78 BiP/HSPA5 antibody PA1815. Let me know if you need anything else.
J. Miller
Verified customer
Asked: 2013-08-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-06
