Anti-Calreticulin Rabbit Monoclonal Antibody

Calreticulin antibody

Boster Bio Anti-Calreticulin Rabbit Monoclonal Antibody catalog # M00894. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00894
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Calreticulin Rabbit Monoclonal Antibody

View all Calreticulin Antibodies

SKU/Catalog Number

M00894

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Calreticulin Rabbit Monoclonal Antibody catalog # M00894. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calreticulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00894)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CGO-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Calreticulin - ER Marker

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00894 is reactive to CALR in Human, Mouse, Rat

Applications

M00894 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

48.142kDa

Background of Calreticulin

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CALR (Source: Uniprot.org, NCBI)

Gene Name

CALR

Full Name

Calreticulin

Weight

48.142kDa

Superfamily

calreticulin family

Alternative Names

Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; ERp60; FLJ26680; grp60; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; SSAHACBP; Vasostatin CALR CRT, HEL-S-99n, RO, SSA, cC1qR calreticulin calreticulin|CRP55|ERp60|HACBP|Sicca syndrome antigen A (autoantigen Ro; calreticulin)|calregulin|endoplasmic reticulum resident protein 60|epididymis secretory sperm binding protein Li 99n|grp60

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CALR, check out the CALR Infographic

CALR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CALR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00894 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.

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15 Customer Q&As for Anti-Calreticulin Rabbit Monoclonal Antibody

Question

Will M00894 anti-Calreticulin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

It shows on the product datasheet, M00894 anti-Calreticulin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-30

Question

I am looking for to test anti-Calreticulin Rabbit Monoclonal antibody M00894 on mouse cajal-retzius cell fetal brain cortex for research purposes, then I may be interested in using anti-Calreticulin Rabbit Monoclonal antibody M00894 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The products we sell, including anti-Calreticulin Rabbit Monoclonal antibody M00894, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-16

Question

I was wanting to use your anti-Calreticulin Rabbit Monoclonal antibody for IF for mouse cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cajal-retzius cell fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

As indicated on the product datasheet, M00894 anti-Calreticulin Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-05

Question

We purchased anti-Calreticulin Rabbit Monoclonal antibody for WB on placenta a few years ago. I am using rat, and We are going to use the antibody for IF next. We want examining placenta as well as colon carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

I have checked the website and datasheets of our anti-Calreticulin Rabbit Monoclonal antibody and it seems that M00894 has been validated on rat in both WB and IF. Thus M00894 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-04

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Calreticulin Rabbit Monoclonal antibody M00894. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-04

Question

Do you have a BSA free version of anti-Calreticulin Rabbit Monoclonal antibody M00894 available?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calreticulin Rabbit Monoclonal antibody M00894 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-09

Question

Is this M00894 anti-Calreticulin Rabbit Monoclonal antibody reactive to the isotypes of CALR?

G. Huang

Verified customer

Asked: 2019-09-16

Answer

The immunogen of M00894 anti-Calreticulin Rabbit Monoclonal antibody is A synthesized peptide derived from human Calreticulin - ER Marker. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-16

Question

See below the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Calreticulin Rabbit Monoclonal antibody M00894. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-23

Question

Is a blocking peptide available for product anti-Calreticulin Rabbit Monoclonal antibody (M00894)?

D. Zhang

Verified customer

Asked: 2019-07-16

Answer

We do provide the blocking peptide for product anti-Calreticulin Rabbit Monoclonal antibody (M00894). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-16

Question

We have observed staining in human pancreas skin. Any tips? Is anti-Calreticulin Rabbit Monoclonal antibody supposed to stain pancreas skin positively?

Verified Customer

Verified customer

Asked: 2019-02-05

Answer

According to literature pancreas skin does express CALR. According to Uniprot.org, CALR is expressed in thyroid gland, eye, pancreas skin, placenta, liver, brain, cajal-retzius cell fetal brain cortex, keratinocyte, colon carcinoma, among other tissues. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874

Boster Scientific Support

Answered: 2019-02-05

Question

My team were content with the WB result of your anti-Calreticulin Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is CALR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-02-04

Answer

From literature, placenta does express CALR. Generally CALR expresses in endoplasmic reticulum lumen. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874

Boster Scientific Support

Answered: 2019-02-04

Question

Can you help my question with product M00894, anti-Calreticulin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-17

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00894 anti-Calreticulin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-17

Question

Would anti-Calreticulin Rabbit Monoclonal antibody M00894 work for IF with cajal-retzius cell fetal brain cortex?

P. Li

Verified customer

Asked: 2015-06-18

Answer

According to the expression profile of cajal-retzius cell fetal brain cortex, CALR is highly expressed in cajal-retzius cell fetal brain cortex. So, it is likely that anti-Calreticulin Rabbit Monoclonal antibody M00894 will work for IF with cajal-retzius cell fetal brain cortex.

Boster Scientific Support

Answered: 2015-06-18

Question

I see that the anti-Calreticulin Rabbit Monoclonal antibody M00894 works with IF, what is the protocol used to produce the result images on the product page?

O. Krishna

Verified customer

Asked: 2014-10-24

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-10-24

Question

We are currently using anti-Calreticulin Rabbit Monoclonal antibody M00894 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Z. Patel

Verified customer

Asked: 2014-04-10

Answer

The anti-Calreticulin Rabbit Monoclonal antibody (M00894) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-10

Order DetailsPrice
M00894

100μL

$370
M00894-30ul

30μL sample

$99
M00894-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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