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Product Info Summary
| SKU: | PA1440 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-1(P20)/CASP1 Antibody Picoband®
SKU/Catalog Number
PA1440
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-1 (P20)/CASP1 Antibody catalog # PA1440. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-1(P20)/CASP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1440)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CASP1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1440 is reactive to CASP1 in Human
Observed Molecular Weight
45 kDa
Calculated molecular weight
45.2 kDa
Background of CASP1
Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1440 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Cell, RAJI Cell, CEM Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-Caspase-1(P20) antibody, PA1440, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Caspase-1(P20) antibody, PA1440, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 3: CEM Cell Lysate
Specific Publications For Anti-Caspase-1(P20)/CASP1 Antibody Picoband® (PA1440)
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Customer Q&As
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17 Customer Q&As for Anti-Caspase-1(P20)/CASP1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Caspase-1(P20)/CASP1 antibody (PA1440)?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We do provide the blocking peptide for product anti-Caspase-1(P20)/CASP1 antibody (PA1440). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-03
Question
Does anti-Caspase-1(P20)/CASP1 antibody PA1440 work for IHC with macrophage?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of macrophage, CASP1 is highly expressed in macrophage. So, it is likely that anti-Caspase-1(P20)/CASP1 antibody PA1440 will work for IHC with macrophage.
Boster Scientific Support
Answered: 2020-02-11
Question
We have observed staining in human macrophage. What should we do? Is anti-Caspase-1(P20)/CASP1 antibody supposed to stain macrophage positively?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Based on literature macrophage does express CASP1. Based on Uniprot.org, CASP1 is expressed in leukocyte, testis, macrophage, among other tissues. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-02
Question
We purchased anti-Caspase-1(P20)/CASP1 antibody for IHC on leukocyte a few months ago. I am using human, and I plan to use the antibody for WB next. We are interested in examining leukocyte as well as macrophage in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
I have checked the website and datasheets of our anti-Caspase-1(P20)/CASP1 antibody and it seems that PA1440 has been tested on human in both IHC and WB. Thus PA1440 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-10
Question
I have attached the WB image, lot number and protocol we used for macrophage using anti-Caspase-1(P20)/CASP1 antibody PA1440. Please let me know if you require anything else.
N. Patel
Verified customer
Asked: 2019-11-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-26
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-Caspase-1(P20)/CASP1 antibody PA1440. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
Does PA1440 anti-Caspase-1(P20)/CASP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can see on the product datasheet, PA1440 anti-Caspase-1(P20)/CASP1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-07
Question
We are currently using anti-Caspase-1(P20)/CASP1 antibody PA1440 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
V. Krishna
Verified customer
Asked: 2019-09-19
Answer
The anti-Caspase-1(P20)/CASP1 antibody (PA1440) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
My question regards to test anti-Caspase-1(P20)/CASP1 antibody PA1440 on human macrophage for research purposes, then I may be interested in using anti-Caspase-1(P20)/CASP1 antibody PA1440 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-Caspase-1(P20)/CASP1 antibody PA1440, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this PA1440 anti-Caspase-1(P20)/CASP1 antibody reactive to the isotypes of CASP1?
K. Jones
Verified customer
Asked: 2019-05-08
Answer
The immunogen of PA1440 anti-Caspase-1(P20)/CASP1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CASP1(135-152aa CSLEEAQRIWKQKSAEIY). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-08
Question
I was wanting to use your anti-Caspase-1(P20)/CASP1 antibody for IHC for human macrophage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human macrophage identification?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
It shows on the product datasheet, PA1440 anti-Caspase-1(P20)/CASP1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-17
Question
Has PA1522 and PA1440 been tested for IHC-F on human samples?
Verified customer
Asked: 2019-03-29
Answer
Unfortunately, we have not tested the Anti-Caspase-1(P10)/CASP1 Antibody (PA1522) and Anti-Caspase-1(P20)/CASP1 Antibody (PA1440) for IHC-F on human samples. So, we do not know if they will work for IHC-F.
Boster Scientific Support
Answered: 2019-04-01
Question
Do you have a BSA free version of anti-Caspase-1(P20)/CASP1 antibody PA1440 available?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-1(P20)/CASP1 antibody PA1440 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-15
Question
I am interested in using your anti-Caspase-1(P20)/CASP1 antibody for interleukin-1 processing studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
We appreciate your inquiry. This PA1440 anti-Caspase-1(P20)/CASP1 antibody is tested on jurkat cell lysate, raji cell lysate, cem cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-16
Question
I see that the anti-Caspase-1(P20)/CASP1 antibody PA1440 works with IHC, what is the protocol used to produce the result images on the product page?
N. Li
Verified customer
Asked: 2016-06-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-06-07
Question
My question regarding product PA1440, anti-Caspase-1(P20)/CASP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Williams
Verified customer
Asked: 2014-12-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1440 anti-Caspase-1(P20)/CASP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-30
Question
My team were satisfied with the WB result of your anti-Caspase-1(P20)/CASP1 antibody. However we have observed positive staining in testis cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP1 supposed to be expressed?
B. Krishna
Verified customer
Asked: 2014-06-16
Answer
Based on literature, testis does express CASP1. Generally CASP1 expresses in cytoplasm. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-06-16
