Anti-Caspase-3/CASP3 Antibody Picoband®

Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 35 kDa, 17 kDa (Cleaved). The expected band size for Caspase-3 is at 32 kDa.

Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 35 kDa, 17 kDa (Cleaved). The expected band size for Caspase-3 is at 32 kDa.

IHC analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188).
Caspase-3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase-3 Antibody (PB9188) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188).
Caspase-3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Caspase-3 Antibody (PB9188) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Regulatory effects of KSG on the expression of cytokines and apoptosis-related factors in Aβ-induced PC12 cells. (A) Expression of IL-1β (n = 3). (B) Expression of IL-18 (n = 3). (C) Expression of TNF-α (n = 3). (D) Protein expression of apoptosis-related factors in PC12 cells of each group (n = 3). (E) Expression of Caspase-3 (n = 3). (F) Expression of Bax (n = 3). Data were expressed as mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. Con. # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. AD.
Index in PubMed under a CC BY license. PMID: 41050397