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Product Info Summary
| SKU: | FEK1117 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Elafin/PI3 PicoKine® Quick ELISA Kit
See all Trappin-2/Elafin/Skalp products
SKU/Catalog Number
FEK1117
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Elafin/PI3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human PI3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Elafin/PI3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1117)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A23-Q117
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1117 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1117 is reactive to PI3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1117 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Trappin-2/Elafin/Skalp
Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1117-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1117-ST | Human Trappin-2/Elafin/Skalp Standard | 2 | 10 ng/tube |
| FEK1117-A | Human PI3 Antibody A | 1 | 3ml |
| FEK1117-B | Human PI3 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.024 | 0.154 | 0.244 | 0.461 | 0.821 | 1.425 | 2.122 | 2.274 |
Data Example Images
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Human Elafin/PI3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 67 | 269 | 902 | 75 | 263 | 887 |
| Standard deviation | 4.36 | 12.91 | 50.51 | 5.33 | 14.73 | 60.32 |
| CV (%) | 6.5% | 4.8% | 5.6% | 7.1% | 5.6% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Trappin-2/Elafin/Skalp in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 67 | 55 | 59 | 53 | 59 | 3.80 | 6.5% |
| Sample 2 | 269 | 260 | 270 | 259 | 265 | 11.37 | 4.3% |
| Sample 3 | 902 | 996 | 955 | 1033 | 972 | 56.35 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For PI3 (Source: Uniprot.Org, NCBI)
Gene Name
PI3
Full Name
Elafin
Weight
12.27kDa
Alternative Names
cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin PI3 ESI, SKALP, WAP3, WFDC14, cementoin peptidase inhibitor 3 elafin|PI-3|WAP four-disulfide core domain 14|WAP four-disulfide core domain protein 14|elastase-specific inhibitor|peptidase inhibitor 3, skin-derived|pre-elafin|protease inhibitor 3, skin-derived (SKALP)|protease inhibitor WAP3|skin-derived antileukoproteinase|trappin-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PI3, check out the PI3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PI3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Elafin/PI3 PicoKine® Quick ELISA Kit (FEK1117)
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