Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9/Casp9 antibody (A00080-7).
Overlay histogram showing mouse spleen tissue stained with A00080-7 (Blue line). To facilitate intracellular staining, tissues were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The tissue sections were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/Casp9 Antibody (A00080-7, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.