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Product Info Summary
| SKU: | A01456-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cathepsin B CTSB Antibody
View all Cathepsin B Antibodies
SKU/Catalog Number
A01456-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin B CTSB Antibody catalog # A01456-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin B CTSB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01456-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
3B5
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Cathepsin B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01456-1 is reactive to CTSB in Human, Mouse, Rat
Applications
A01456-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
37.822kDa
Background of Cathepsin B
DDB2 is also known as Damage-specific DNA binding protein 2, DDB p48 subunit, DDBb, UV-damaged DNA-binding protein 2 and UV-DDB 2. The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Cathepsin B Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CTSB (Source: Uniprot.org, NCBI)
Gene Name
CTSB
Full Name
Cathepsin B
Weight
37.822kDa
Superfamily
peptidase C1 family
Alternative Names
APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1 CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTSB, check out the CTSB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin B CTSB Antibody (A01456-1)
Hello CJ!
A01456-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Elimination of Intracellular Calcium Overload by BAPTA-AM-Loaded Liposomes: A Promising Therapeutic Agent for Acute Liver Failure
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Cathepsin B CTSB Antibody
Question
I see that the anti-Cathepsin B antibody A01456-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-05-07
Question
Does anti-Cathepsin B antibody A01456-1 work for WB with melanoma?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of melanoma, CTSB is highly expressed in melanoma. So, it is likely that anti-Cathepsin B antibody A01456-1 will work for WB with melanoma.
Boster Scientific Support
Answered: 2019-08-12
Question
We were satisfied with the WB result of your anti-Cathepsin B antibody. However we have observed positive staining in melanoma lysosome. using this antibody. Is that expected? Could you tell me where is CTSB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
According to literature, melanoma does express CTSB. Generally CTSB expresses in lysosome. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2019-07-18
Question
Do you have a BSA free version of anti-Cathepsin B antibody A01456-1 available?
G. Johnson
Verified customer
Asked: 2019-06-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin B antibody A01456-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-28
Question
See below the WB image, lot number and protocol we used for melanoma using anti-Cathepsin B antibody A01456-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-Cathepsin B antibody for WB for human melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human melanoma identification?
O. Zhang
Verified customer
Asked: 2016-05-27
Answer
As indicated on the product datasheet, A01456-1 anti-Cathepsin B antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-27
Question
Does A01456-1 anti-Cathepsin B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Wu
Verified customer
Asked: 2016-03-30
Answer
As indicated on the product datasheet, A01456-1 anti-Cathepsin B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-30
Question
Is this A01456-1 anti-Cathepsin B antibody reactive to the isotypes of CTSB?
M. Thomas
Verified customer
Asked: 2015-03-31
Answer
The immunogen of A01456-1 anti-Cathepsin B antibody is Synthesized peptide derived from the Internal region of human Cathepsin B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-Cathepsin B antibody A01456-1. Let me know if you need anything else.
T. Carter
Verified customer
Asked: 2014-12-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-17
Question
I am interested in to test anti-Cathepsin B antibody A01456-1 on human melanoma for research purposes, then I may be interested in using anti-Cathepsin B antibody A01456-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Baker
Verified customer
Asked: 2014-12-01
Answer
The products we sell, including anti-Cathepsin B antibody A01456-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-01
Question
We are currently using anti-Cathepsin B antibody A01456-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
C. Krishna
Verified customer
Asked: 2014-06-05
Answer
The anti-Cathepsin B antibody (A01456-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-05
Question
We have observed staining in human thyroid gland. What should we do? Is anti-Cathepsin B antibody supposed to stain thyroid gland positively?
E. Lewis
Verified customer
Asked: 2014-06-03
Answer
From what I have seen in literature thyroid gland does express CTSB. From what I have seen in Uniprot.org, CTSB is expressed in thyroid gland, gastric carcinoma, esophagus, brain placenta, liver, melanoma, among other tissues. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2014-06-03
Question
I have a question about product A01456-1, anti-Cathepsin B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Patel
Verified customer
Asked: 2013-11-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01456-1 anti-Cathepsin B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-29
Question
Is a blocking peptide available for product anti-Cathepsin B antibody (A01456-1)?
H. Walker
Verified customer
Asked: 2013-06-25
Answer
We do provide the blocking peptide for product anti-Cathepsin B antibody (A01456-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-06-25
