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Product Info Summary
| SKU: | A01456-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cathepsin B CTSB Antibody
SKU/Catalog Number
A01456-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin B CTSB Antibody catalog # A01456-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin B CTSB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01456-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Cathepsin B. AA range:168-217
Reactive Species
A01456-1 is reactive to CTSB in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
37.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01456-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Cathepsin B Polyclonal Antibody
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Western blot analysis of lysate from COLO cells, using Cathepsin B antibody.
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Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Immunohistochemistry analysis of Cathepsin B antibody in paraffin-embedded human brain tissue.
Specific Publications For Anti-Cathepsin B CTSB Antibody (A01456-1)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Cathepsin B CTSB Antibody
Question
I see that the anti-Cathepsin B antibody A01456-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-05-07
Question
Does anti-Cathepsin B antibody A01456-1 work for WB with melanoma?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to the expression profile of melanoma, CTSB is highly expressed in melanoma. So, it is likely that anti-Cathepsin B antibody A01456-1 will work for WB with melanoma.
Boster Scientific Support
Answered: 2019-08-12
Question
We were satisfied with the WB result of your anti-Cathepsin B antibody. However we have observed positive staining in melanoma lysosome. using this antibody. Is that expected? Could you tell me where is CTSB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
According to literature, melanoma does express CTSB. Generally CTSB expresses in lysosome. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2019-07-18
Question
Do you have a BSA free version of anti-Cathepsin B antibody A01456-1 available?
G. Johnson
Verified customer
Asked: 2019-06-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin B antibody A01456-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-28
Question
See below the WB image, lot number and protocol we used for melanoma using anti-Cathepsin B antibody A01456-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-Cathepsin B antibody for WB for human melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human melanoma identification?
O. Zhang
Verified customer
Asked: 2016-05-27
Answer
As indicated on the product datasheet, A01456-1 anti-Cathepsin B antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-27
Question
Does A01456-1 anti-Cathepsin B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Wu
Verified customer
Asked: 2016-03-30
Answer
As indicated on the product datasheet, A01456-1 anti-Cathepsin B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-30
Question
Is this A01456-1 anti-Cathepsin B antibody reactive to the isotypes of CTSB?
M. Thomas
Verified customer
Asked: 2015-03-31
Answer
The immunogen of A01456-1 anti-Cathepsin B antibody is Synthesized peptide derived from the Internal region of human Cathepsin B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-Cathepsin B antibody A01456-1. Let me know if you need anything else.
T. Carter
Verified customer
Asked: 2014-12-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-17
Question
I am interested in to test anti-Cathepsin B antibody A01456-1 on human melanoma for research purposes, then I may be interested in using anti-Cathepsin B antibody A01456-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Baker
Verified customer
Asked: 2014-12-01
Answer
The products we sell, including anti-Cathepsin B antibody A01456-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-12-01
Question
We are currently using anti-Cathepsin B antibody A01456-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
C. Krishna
Verified customer
Asked: 2014-06-05
Answer
The anti-Cathepsin B antibody (A01456-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-05
Question
We have observed staining in human thyroid gland. What should we do? Is anti-Cathepsin B antibody supposed to stain thyroid gland positively?
E. Lewis
Verified customer
Asked: 2014-06-03
Answer
From what I have seen in literature thyroid gland does express CTSB. From what I have seen in Uniprot.org, CTSB is expressed in thyroid gland, gastric carcinoma, esophagus, brain placenta, liver, melanoma, among other tissues. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2014-06-03
Question
I have a question about product A01456-1, anti-Cathepsin B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Patel
Verified customer
Asked: 2013-11-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01456-1 anti-Cathepsin B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-29
Question
Is a blocking peptide available for product anti-Cathepsin B antibody (A01456-1)?
H. Walker
Verified customer
Asked: 2013-06-25
Answer
We do provide the blocking peptide for product anti-Cathepsin B antibody (A01456-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-06-25
