Human Cathepsin B ELISA Kit PicoKine™

Human Cathepsin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Product Info Summary

SKU: EK0670
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Cathepsin B ELISA Kit PicoKine™

See all Cathepsin B products

SKU/Catalog Number

EK0670

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Cathepsin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Cathepsin B ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0670)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R18-I339

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0670 is reactive to CTSB in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0670 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0670-CAP Anti-Human CTSB Pre-coated 96-well strip microplate 1
EK0670-ST Human CTSB Standard 2 vials, 10 ng/tube
EK0670-DA Human CTSB Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0480.2170.2990.5220.8971.0741.5481.923

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cathepsin B, Dilution ratio of 1:40, concentration in serum is around 100ng/ml.

Some articles we found to cite concentrations of Cathepsin B in samples: 22848251 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Cathepsin B ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2521856429623419234064
Standard deviation17.64129.92326.4917.85148.07377.95
CV (%)7%7%7.6%7.8%7.7%9.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cathepsin B in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 125227523527225816.196.2%
Sample 21856177616911826178762.483.4%
Sample 342964210383246174238279.566.5%
*number of samples for each test n=16.

Gene/Protein Information For CTSB (Source: Uniprot.Org, NCBI)

Uniprot ID

P07858

Gene ID

1508

Gene Name

CTSB

Full Name

Cathepsin B

Weight

37.822kDa

Superfamily

peptidase C1 family

Alternative Names

APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1 CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTSB, check out the CTSB Infographic

CTSB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTSB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK0670

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9 Customer Q&As for Human Cathepsin B ELISA Kit PicoKine™

Question

Will the phenol red in the media interfere the assay when using EK0670 with cell culture supernatant?

Verified customer

Asked: 2021-01-04

Answer

Phenol red in the media will not interfere with the assay.

Boster Scientific Support

Answered: 2021-01-04

Question

Q: Can Cathepsin B ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-10-04

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-10-04

Question

Do you have any data available from actual human plasma or serum testing on this kit? Keyword: results, data

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We detected healthy human plasma/serum samples and the concentration is about 32-137 ng/ml, and the recovery range is about 97%-112%.

Boster Scientific Support

Answered: 2020-04-09

Question

Dear Support: Just an inquiry about the Human Cathepsin B ELISA Kit PicoKine does this antibody detects mature form of Cathepsin B or the uncleaved form or both? Also is the kit good for cell lines samples?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

The product Human Cathepsin B ELISA Kit PicoKine can detect both mature form and uncleaved form of Cathepasin B. This antibody have been found to work well in serum, cell culture supernatant, plasma and tissue extracts.

Boster Scientific Support

Answered: 2019-09-12

Question

Q: if the enzyme conjugated Cathepsin B antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cathepsin B antigen?

Verified Customer

Verified customer

Asked: 2019-05-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-05-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-02-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-02-02

Question

Q: is it okay to use citrate plasma as samples in Human Cathepsin B Picokine® ELISA Kit (Catalog # EK0670)?

Verified Customer

Verified customer

Asked: 2018-10-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Cathepsin B causing disruption of its protein structure. Cathepsin B may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Cathepsin B ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-10-19

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-08-02

Question

Q: can you suggest the dilution ratio of serum samples for detection of Cathepsin B in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-03-25

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cathepsin B ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-03-25

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