Product Info Summary
SKU: | M01456 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody
View all Cathepsin B Antibodies
SKU/Catalog Number
M01456
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody catalog # M01456. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01456)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cathepsin B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01456 is reactive to CTSB in Human
Applications
M01456 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
37.822kDa
Background of Cathepsin B
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cathepsin B using anti-Cathepsin B antibody (M01456).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin B antigen affinity purified monoclonal antibody (Catalog # M01456) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin B at approximately 38 kDa. The expected band size for Cathepsin B is at 38 kDa.
Click image to see more details
Figure 2. IHC analysis of Cathepsin B using anti-Cathepsin B antibody (M01456).
Cathepsin B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Cathepsin B Antibody M01456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Cathepsin B using anti-Cathepsin B antibody (M01456).
Cathepsin B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Cathepsin B Antibody M01456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Cathepsin B using anti-Cathepsin B antibody (M01456).
Cathepsin B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Cathepsin B Antibody M01456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CTSB (Source: Uniprot.org, NCBI)
Gene Name
CTSB
Full Name
Cathepsin B
Weight
37.822kDa
Superfamily
peptidase C1 family
Alternative Names
APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1 CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTSB, check out the CTSB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (M01456)
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No publications found for M01456
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
15 Customer Q&As for Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody
Question
We bought anti-Cathepsin B Rabbit Monoclonal antibody for IP on melanoma a few months ago. I am using human, and We want to use the antibody for WB next. I was wanting to use examining melanoma as well as esophagus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
I looked at the website and datasheets of our anti-Cathepsin B Rabbit Monoclonal antibody and it appears that M01456 has been validated on human in both IP and WB. Thus M01456 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-10
Question
I was wanting to use your anti-Cathepsin B Rabbit Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It shows on the product datasheet, M01456 anti-Cathepsin B Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-23
Question
Is this M01456 anti-Cathepsin B Rabbit Monoclonal antibody reactive to the isotypes of CTSB?
K. Krishna
Verified customer
Asked: 2019-10-24
Answer
The immunogen of M01456 anti-Cathepsin B Rabbit Monoclonal antibody is A synthesized peptide derived from human Cathepsin B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-Cathepsin B Rabbit Monoclonal antibody M01456 available?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin B Rabbit Monoclonal antibody M01456 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-02
Question
Is a blocking peptide available for product anti-Cathepsin B Rabbit Monoclonal antibody (M01456)?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We do provide the blocking peptide for product anti-Cathepsin B Rabbit Monoclonal antibody (M01456). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-05
Question
We need to test anti-Cathepsin B Rabbit Monoclonal antibody M01456 on human liver for research purposes, then I may be interested in using anti-Cathepsin B Rabbit Monoclonal antibody M01456 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The products we sell, including anti-Cathepsin B Rabbit Monoclonal antibody M01456, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-22
Question
Can you help my question with product M01456, anti-Cathepsin B Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01456 anti-Cathepsin B Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-Cathepsin B Rabbit Monoclonal antibody M01456 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
A. Patel
Verified customer
Asked: 2019-05-09
Answer
The anti-Cathepsin B Rabbit Monoclonal antibody (M01456) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-09
Question
Please see the WB image, lot number and protocol we used for liver using anti-Cathepsin B Rabbit Monoclonal antibody M01456. Please let me know if you require anything else.
M. Zhang
Verified customer
Asked: 2018-05-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-11
Question
Will anti-Cathepsin B Rabbit Monoclonal antibody M01456 work for IP with liver?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
According to the expression profile of liver, CTSB is highly expressed in liver. So, it is likely that anti-Cathepsin B Rabbit Monoclonal antibody M01456 will work for IP with liver.
Boster Scientific Support
Answered: 2017-12-27
Question
My team were happy with the WB result of your anti-Cathepsin B Rabbit Monoclonal antibody. However we have seen positive staining in brain placenta lysosome. using this antibody. Is that expected? Could you tell me where is CTSB supposed to be expressed?
M. Brown
Verified customer
Asked: 2017-07-07
Answer
Based on literature, brain placenta does express CTSB. Generally CTSB expresses in lysosome. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2017-07-07
Question
I see that the anti-Cathepsin B Rabbit Monoclonal antibody M01456 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-02
Question
Will M01456 anti-Cathepsin B Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
As indicated on the product datasheet, M01456 anti-Cathepsin B Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-15
Question
We have observed staining in human gastric carcinoma. Any tips? Is anti-Cathepsin B Rabbit Monoclonal antibody supposed to stain gastric carcinoma positively?
V. Bhatt
Verified customer
Asked: 2016-07-22
Answer
Based on literature gastric carcinoma does express CTSB. Based on Uniprot.org, CTSB is expressed in thyroid gland, gastric carcinoma, esophagus, brain placenta, liver, melanoma, among other tissues. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065
Boster Scientific Support
Answered: 2016-07-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cathepsin B Rabbit Monoclonal antibody M01456. Let me know if you need anything else.
R. Li
Verified customer
Asked: 2014-06-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-23