Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody

Boster Bio Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody catalog # M01456. Tested in IP, IF, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M01456
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody

See all Cathepsin B products

SKU/Catalog Number

M01456

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody catalog # M01456. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01456)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAFO-3

Immunogen

A synthesized peptide derived from human Cathepsin B

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01456 is reactive to CTSB in Human

Applications

M01456 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CTSB (Source: Uniprot.Org, NCBI)

Uniprot ID

P07858

Gene ID

1508

Gene Name

CTSB

Full Name

Cathepsin B

Weight

37.822kDa

Superfamily

peptidase C1 family

Alternative Names

APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1 CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTSB, check out the CTSB Infographic

CTSB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTSB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01456

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Find answers in Q&As, reviews.

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15 Customer Q&As for Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody

Question

We bought anti-Cathepsin B Rabbit Monoclonal antibody for IP on melanoma a few months ago. I am using human, and We want to use the antibody for WB next. I was wanting to use examining melanoma as well as esophagus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

I looked at the website and datasheets of our anti-Cathepsin B Rabbit Monoclonal antibody and it appears that M01456 has been validated on human in both IP and WB. Thus M01456 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-10

Question

I was wanting to use your anti-Cathepsin B Rabbit Monoclonal antibody for IP for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

It shows on the product datasheet, M01456 anti-Cathepsin B Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-23

Question

Is this M01456 anti-Cathepsin B Rabbit Monoclonal antibody reactive to the isotypes of CTSB?

K. Krishna

Verified customer

Asked: 2019-10-24

Answer

The immunogen of M01456 anti-Cathepsin B Rabbit Monoclonal antibody is A synthesized peptide derived from human Cathepsin B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-24

Question

Do you have a BSA free version of anti-Cathepsin B Rabbit Monoclonal antibody M01456 available?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin B Rabbit Monoclonal antibody M01456 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-02

Question

Is a blocking peptide available for product anti-Cathepsin B Rabbit Monoclonal antibody (M01456)?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

We do provide the blocking peptide for product anti-Cathepsin B Rabbit Monoclonal antibody (M01456). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-05

Question

We need to test anti-Cathepsin B Rabbit Monoclonal antibody M01456 on human liver for research purposes, then I may be interested in using anti-Cathepsin B Rabbit Monoclonal antibody M01456 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

The products we sell, including anti-Cathepsin B Rabbit Monoclonal antibody M01456, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-22

Question

Can you help my question with product M01456, anti-Cathepsin B Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01456 anti-Cathepsin B Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-27

Question

We are currently using anti-Cathepsin B Rabbit Monoclonal antibody M01456 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

A. Patel

Verified customer

Asked: 2019-05-09

Answer

The anti-Cathepsin B Rabbit Monoclonal antibody (M01456) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-09

Question

Please see the WB image, lot number and protocol we used for liver using anti-Cathepsin B Rabbit Monoclonal antibody M01456. Please let me know if you require anything else.

M. Zhang

Verified customer

Asked: 2018-05-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-11

Question

Will anti-Cathepsin B Rabbit Monoclonal antibody M01456 work for IP with liver?

Verified Customer

Verified customer

Asked: 2017-12-27

Answer

According to the expression profile of liver, CTSB is highly expressed in liver. So, it is likely that anti-Cathepsin B Rabbit Monoclonal antibody M01456 will work for IP with liver.

Boster Scientific Support

Answered: 2017-12-27

Question

My team were happy with the WB result of your anti-Cathepsin B Rabbit Monoclonal antibody. However we have seen positive staining in brain placenta lysosome. using this antibody. Is that expected? Could you tell me where is CTSB supposed to be expressed?

M. Brown

Verified customer

Asked: 2017-07-07

Answer

Based on literature, brain placenta does express CTSB. Generally CTSB expresses in lysosome. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065

Boster Scientific Support

Answered: 2017-07-07

Question

I see that the anti-Cathepsin B Rabbit Monoclonal antibody M01456 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-02

Question

Will M01456 anti-Cathepsin B Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-15

Answer

As indicated on the product datasheet, M01456 anti-Cathepsin B Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-15

Question

We have observed staining in human gastric carcinoma. Any tips? Is anti-Cathepsin B Rabbit Monoclonal antibody supposed to stain gastric carcinoma positively?

V. Bhatt

Verified customer

Asked: 2016-07-22

Answer

Based on literature gastric carcinoma does express CTSB. Based on Uniprot.org, CTSB is expressed in thyroid gland, gastric carcinoma, esophagus, brain placenta, liver, melanoma, among other tissues. Regarding which tissues have CTSB expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Esophagus, Pubmed ID: 14702039
Gastric carcinoma, Pubmed ID: 8112600
Liver, Pubmed ID: 1637335, 3972105, 24275569
Melanoma, Pubmed ID: 12643545, 17081065

Boster Scientific Support

Answered: 2016-07-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cathepsin B Rabbit Monoclonal antibody M01456. Let me know if you need anything else.

R. Li

Verified customer

Asked: 2014-06-23

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-23

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M01456
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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