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- Table of Contents
CTSB antibody (M01456) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of Cathepsin B using anti-Cathepsin B antibody (M01456).
Cathepsin B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Cathepsin B Antibody M01456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cathepsin B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Cathepsin B Antibody M01456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody
CTSB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CTSB antibody was positively detected by WB in:
human U-87MG whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTSB antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human liver cancer tissue, human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody
View all CTSB Antibodies
View all CTSB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cathepsin B
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
37.8 kDa
Cite This Product
Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01456)
Protocols
Western Blot protocol for CTSB antibody
Immunohistochemistry (IHC) protocol for CTSB antibody
Publications
Application:
Species:
KNSTRN knockdown impairs autophagy flux to inhibit bladder cancer progression
Published: 2026-01-19
Journal: iScience
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 41704764
Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis
Published: 2024-02-26
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 1.50
Species: Chicken
PMID: 38427138
Metformin suppresses vascular smooth muscle cell senescence by promoting autophagic flux
Published: 2021-12-22
Journal: Journal of Advanced Research
Application: WB
Impact Factor: 10.48
Species: Human
PMID: 36328749
Review
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Product Promise
We promise Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M01456
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026