Anti-Cathepsin D/CTSD Antibody Picoband™

Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband™ catalog # PB9047. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9047
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Cathepsin D/CTSD Antibody Picoband™

See all Cathepsin D products

SKU/Catalog Number

PB9047

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband™ catalog # PB9047. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cathepsin D/CTSD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9047)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Cathepsin D recombinant protein (Position: G65-L412). Human Cathepsin D shares 85% amino acid (aa) sequence identity with both mouse and rat Cathepsin D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9047 is reactive to CTSD in Human

Applications

PB9047 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CTSD (Source: Uniprot.Org, NCBI)

Uniprot ID

P07339

Gene ID

1509

Gene Name

CTSD

Full Name

Cathepsin D

Weight

44.552kDa

Superfamily

peptidase A1 family

Alternative Names

cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10 CTSD CLN10, CPSD, HEL-S-130P cathepsin D cathepsin D|ceroid-lipofuscinosis, neuronal 10|epididymis secretory sperm binding protein Li 130P|lysosomal aspartyl peptidase|lysosomal aspartyl protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CTSD, check out the CTSD Infographic

CTSD infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CTSD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9047 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint

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5 Customer Q&As for Anti-Cathepsin D/CTSD Antibody Picoband™

Question

I was wanting to use using your anti-Cathepsin D/CTSD antibody for collagen degradation studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

Thank you for your inquiry. This PB9047 anti-Cathepsin D/CTSD antibody is validated on lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-24

Question

We have seen staining in human liver. Are there any suggestions? Is anti-Cathepsin D/CTSD antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

Based on literature liver does express CTSD. Based on Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2020-02-14

Question

We are currently using anti-Cathepsin D/CTSD antibody PB9047 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The anti-Cathepsin D/CTSD antibody (PB9047) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-13

Question

We ordered your anti-Cathepsin D/CTSD antibody for WB on platelet in a previous experiment. I am using human, and We intend to use the antibody for IHC next. I am interested in examining platelet as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

I looked at the website and datasheets of our anti-Cathepsin D/CTSD antibody and it appears that PB9047 has been validated on human in both WB and IHC. Thus PB9047 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-01

Question

We were well pleased with the WB result of your anti-Cathepsin D/CTSD antibody. However we have seen positive staining in spleen lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?

K. Krishna

Verified customer

Asked: 2017-01-02

Answer

From literature, spleen does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2017-01-02

Conjugation

Size

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PB9047
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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