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Product Info Summary
| SKU: | PB9047 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cathepsin D/CTSD Antibody Picoband®
SKU/Catalog Number
PB9047
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband® catalog # PB9047. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin D/CTSD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9047)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin D recombinant protein (Position: G65-L412). Human Cathepsin D shares 85% amino acid (aa) sequence identity with both mouse and rat Cathepsin D.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9047 is reactive to CTSD in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
44.6 kDa
Background of CTSD
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9047 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047).
Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cathepsin D Antibody (PB9047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human Cathepsin D protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # PB9047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 40 kDa. The expected band size for Cathepsin D is at 40 kDa.
Specific Publications For Anti-Cathepsin D/CTSD Antibody Picoband® (PB9047)
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Customer Reviews
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5 Customer Q&As for Anti-Cathepsin D/CTSD Antibody Picoband®
Question
I was wanting to use using your anti-Cathepsin D/CTSD antibody for collagen degradation studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
Thank you for your inquiry. This PB9047 anti-Cathepsin D/CTSD antibody is validated on lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-24
Question
We have seen staining in human liver. Are there any suggestions? Is anti-Cathepsin D/CTSD antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Based on literature liver does express CTSD. Based on Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-Cathepsin D/CTSD antibody PB9047 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-Cathepsin D/CTSD antibody (PB9047) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
We ordered your anti-Cathepsin D/CTSD antibody for WB on platelet in a previous experiment. I am using human, and We intend to use the antibody for IHC next. I am interested in examining platelet as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I looked at the website and datasheets of our anti-Cathepsin D/CTSD antibody and it appears that PB9047 has been validated on human in both WB and IHC. Thus PB9047 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-01
Question
We were well pleased with the WB result of your anti-Cathepsin D/CTSD antibody. However we have seen positive staining in spleen lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?
K. Krishna
Verified customer
Asked: 2017-01-02
Answer
From literature, spleen does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2017-01-02
