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Product Info Summary
| SKU: | M01361 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody
SKU/Catalog Number
M01361
BM4214 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody catalog # M01361. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01361)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CEF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cathepsin D
Reactive Species
M01361 is reactive to CTSD in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
44.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01361 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:20
Positive Control
WB: human MCF-7 whole cell,
human Jurkat whole cell,
human HepG2 whole cell,
human A431 whole cell
IHC: human ovarian cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cathepsin D/CTSD using anti-Cathepsin D/CTSD antibody (M01361).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D/CTSD antigen affinity purified monoclonal antibody (M01361) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D/CTSD at approximately 28 kDa. The expected band size for Cathepsin D/CTSD is at 45 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Cathepsin D Antibody.
Specific Publications For Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody (M01361)
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Customer Q&As
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17 Customer Q&As for Anti-Cathepsin D CTSD Rabbit Monoclonal Antibody
Question
you antibody to test anti-Cathepsin D Rabbit Monoclonal antibody M01361 on rat platelet for research purposes, then I may be interested in using anti-Cathepsin D Rabbit Monoclonal antibody M01361 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The products we sell, including anti-Cathepsin D Rabbit Monoclonal antibody M01361, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-19
Question
Is there a BSA free version of anti-Cathepsin D Rabbit Monoclonal antibody M01361 available?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin D Rabbit Monoclonal antibody M01361 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-11
Question
We have seen staining in rat adult mammalian kidney. Any tips? Is anti-Cathepsin D Rabbit Monoclonal antibody supposed to stain adult mammalian kidney positively?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
From literature adult mammalian kidney does express CTSD. From Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2020-02-11
Question
Our team were happy with the WB result of your anti-Cathepsin D Rabbit Monoclonal antibody. However we have seen positive staining in melanoma lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Based on literature, melanoma does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2020-01-08
Question
Does M01361 anti-Cathepsin D Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
It shows on the product datasheet, M01361 anti-Cathepsin D Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-Cathepsin D Rabbit Monoclonal antibody M01361 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-Cathepsin D Rabbit Monoclonal antibody (M01361) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
Please see the WB image, lot number and protocol we used for platelet using anti-Cathepsin D Rabbit Monoclonal antibody M01361. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-01
Question
Could you help me confirm information regarding Abways products? https://www.bosterbio.com/anti-cathepsin-d-rabbit-monoclonal-antibody-m01361-boster.html?options=cart (is the IHC image of ovary or ovarian cancer?) | Abways CY5339 https://www.bosterbio.com/anti-phospho-lyn-y396-rabbit-monoclonal-antibody-p01424-1-boster.html?options=cart (clone name?) | Abways CY6574
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
For M01361, the IHC image is ovarian cancer. Colne name of P01424-1 is IFI-12.?
Boster Scientific Support
Answered: 2019-06-24
Question
Our lab want to know about using your anti-Cathepsin D Rabbit Monoclonal antibody for mhc class ii antigen presentation studies. Has this antibody been tested with western blotting on ovarian cancer tissue? We would like to see some validation images before ordering.
T. Jackson
Verified customer
Asked: 2019-06-14
Answer
I appreciate your inquiry. This M01361 anti-Cathepsin D Rabbit Monoclonal antibody is validated on ovarian cancer tissue. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-14
Question
Does anti-Cathepsin D Rabbit Monoclonal antibody M01361 work for IF with platelet?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
According to the expression profile of platelet, CTSD is highly expressed in platelet. So, it is likely that anti-Cathepsin D Rabbit Monoclonal antibody M01361 will work for IF with platelet.
Boster Scientific Support
Answered: 2019-06-04
Question
I was wanting to use your anti-Cathepsin D Rabbit Monoclonal antibody for IF for rat platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat platelet identification?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can see on the product datasheet, M01361 anti-Cathepsin D Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Is a blocking peptide available for product anti-Cathepsin D Rabbit Monoclonal antibody (M01361)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We do provide the blocking peptide for product anti-Cathepsin D Rabbit Monoclonal antibody (M01361). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-13
Question
I see that the anti-Cathepsin D Rabbit Monoclonal antibody M01361 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-26
Question
Is this M01361 anti-Cathepsin D Rabbit Monoclonal antibody reactive to the isotypes of CTSD?
S. Li
Verified customer
Asked: 2016-11-23
Answer
The immunogen of M01361 anti-Cathepsin D Rabbit Monoclonal antibody is A synthesized peptide derived from human Cathepsin D. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-11-23
Question
Can you help my question with product M01361, anti-Cathepsin D Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Mangal
Verified customer
Asked: 2014-12-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01361 anti-Cathepsin D Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-22
Question
We bought anti-Cathepsin D Rabbit Monoclonal antibody for IP on melanoma a few years ago. I am using human, and We want to use the antibody for IHC next. you antibody examining melanoma as well as plasma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
S. Huang
Verified customer
Asked: 2014-09-01
Answer
I have checked the website and datasheets of our anti-Cathepsin D Rabbit Monoclonal antibody and I see that M01361 has been validated on human in both IP and IHC. Thus M01361 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-09-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Cathepsin D Rabbit Monoclonal antibody M01361. Let me know if you need anything else.
D. Anderson
Verified customer
Asked: 2013-03-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-29
