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Product Info Summary
| SKU: | A04874 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cathepsin E CTSE Antibody
SKU/Catalog Number
A04874
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cathepsin E CTSE Antibody catalog # A04874. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin E CTSE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04874)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Cathepsin E. AA range:243-292
Reactive Species
A04874 is reactive to CTSE in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
42.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04874 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of Cathepsin E antibody in paraffin-embedded human lung carcinoma tissue.
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Western Blot analysis of various cells using Cathepsin E Polyclonal Antibody
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Western blot analysis of lysate from HUVEC cells, using Cathepsin E antibody.
Specific Publications For Anti-Cathepsin E CTSE Antibody (A04874)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-Cathepsin E CTSE Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastric adenocarcinoma using anti-Cathepsin E antibody A04874. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
Would A04874 anti-Cathepsin E antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
As indicated on the product datasheet, A04874 anti-Cathepsin E antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
I was wanting to use your anti-Cathepsin E antibody for WB for human gastric adenocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human gastric adenocarcinoma identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, A04874 anti-Cathepsin E antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human gastric adenocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
Is a blocking peptide available for product anti-Cathepsin E antibody (A04874)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-Cathepsin E antibody (A04874). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
Is there a BSA free version of anti-Cathepsin E antibody A04874 available?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin E antibody A04874 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-27
Question
Is this A04874 anti-Cathepsin E antibody reactive to the isotypes of CTSE?
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
The immunogen of A04874 anti-Cathepsin E antibody is Synthesized peptide derived from the Internal region of human Cathepsin?E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-17
Question
We need to test anti-Cathepsin E antibody A04874 on human gastric adenocarcinoma for research purposes, then I may be interested in using anti-Cathepsin E antibody A04874 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Jones
Verified customer
Asked: 2018-09-21
Answer
The products we sell, including anti-Cathepsin E antibody A04874, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-21
Question
Will anti-Cathepsin E antibody A04874 work for WB with gastric adenocarcinoma?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
According to the expression profile of gastric adenocarcinoma, CTSE is highly expressed in gastric adenocarcinoma. So, it is likely that anti-Cathepsin E antibody A04874 will work for WB with gastric adenocarcinoma.
Boster Scientific Support
Answered: 2017-10-27
Question
My question regarding product A04874, anti-Cathepsin E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Roberts
Verified customer
Asked: 2015-03-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04874 anti-Cathepsin E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-23
Question
Here is the WB image, lot number and protocol we used for gastric adenocarcinoma using anti-Cathepsin E antibody A04874. Please let me know if you require anything else.
L. Yang
Verified customer
Asked: 2014-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-24
Question
Would anti-Cathepsin E antibody A04874 work on zebrafish WB with gastric mucosa?
T. Rodriguez
Verified customer
Asked: 2014-07-04
Answer
Our lab technicians have not validated anti-Cathepsin E antibody A04874 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Cathepsin E antibody A04874 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish gastric mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-04
Question
I see that the anti-Cathepsin E antibody A04874 works with WB, what is the protocol used to produce the result images on the product page?
D. Johnson
Verified customer
Asked: 2014-04-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-04-01
