Anti-ARID2 Antibody Picoband™

ARID2 antibody

Boster Bio Anti-ARID2 Antibody Picoband™ catalog # A05064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05064
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-ARID2 Antibody Picoband™

View all ARID2 Antibodies

SKU/Catalog Number

A05064

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ARID2 Antibody Picoband™ catalog # A05064. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ARID2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05064)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence of human ARID2 (DERRKGLAFLDELRQFHHSR).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05064 is reactive to ARID2 in Human, Mouse, Rat

Applications

A05064 is guaranteed for IHC, WB Boster Guarantee

Background of ARID2

AT-rich interactive domain-containing protein 2 (ARID2) is a protein that in humans is encoded by the ARID2 gene. It is mapped to 12q12. This gene encodes a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin structure modification. This protein functions as a subunit of the polybromo- and BRG1-associated factor or PBAF (SWI/SNF-B) chromatin remodeling complex which facilitates ligand-dependent transcriptional activation by nuclear receptors. Mutations in this gene are associated with hepatocellular carcinomas. A pseudogene of this gene is found on chromosome1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ARID2 (Source: Uniprot.org, NCBI)

Uniprot ID

Q68CP9

Gene ID

196528

Gene Name

ARID2

Full Name

AT-rich interactive domain-containing protein 2

Weight

197.391kDa

Alternative Names

ARID domain-containing protein 2; AT rich interactive domain 2 (ARID, RFX-like); BAF200KIAA1557AT-rich interactive domain-containing protein 2; BRG1-associated factor 200; DKFZp686G052; DKFZp779P0222; FLJ30619; p200; Zinc finger protein with activation potential; zipzap; zipzap/p200 ARID2 BAF200, CSS6, p200 AT-rich interaction domain 2 AT-rich interactive domain-containing protein 2|ARID domain-containing protein 2|AT rich interactive domain 2 (ARID, RFX-like)|BRG1-associated factor 200|zinc finger protein with activation potential|zipzap/p200

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ARID2, check out the ARID2 Infographic

ARID2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ARID2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

No publications found for A05064

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10 Customer Q&As for Anti-ARID2 Antibody Picoband™

Question

Will anti-ARID2 antibody A05064 work on dog WB with kidney?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

Our lab technicians have not validated anti-ARID2 antibody A05064 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ARID2 antibody A05064 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-17

Question

We are currently using anti-ARID2 antibody A05064 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

The anti-ARID2 antibody (A05064) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-05

Question

Would A05064 anti-ARID2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

You can see on the product datasheet, A05064 anti-ARID2 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-03

Question

Is this A05064 anti-ARID2 antibody reactive to the isotypes of ARID2?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The immunogen of A05064 anti-ARID2 antibody is A synthetic peptide corresponding to a sequence of human ARID2 (DERRKGLAFLDELRQFHHSR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-31

Question

Will anti-ARID2 antibody A05064 work for IHC-P with heart?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

According to the expression profile of heart, ARID2 is highly expressed in heart. So, it is likely that anti-ARID2 antibody A05064 will work for IHC-P with heart.

Boster Scientific Support

Answered: 2019-09-17

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-ARID2 antibody A05064. Let me know if you need anything else.

C. Gonzalez

Verified customer

Asked: 2019-07-23

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-23

Question

Is a blocking peptide available for product anti-ARID2 antibody (A05064)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do provide the blocking peptide for product anti-ARID2 antibody (A05064). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-11

Question

you antibody to test anti-ARID2 antibody A05064 on mouse heart for research purposes, then I may be interested in using anti-ARID2 antibody A05064 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

The products we sell, including anti-ARID2 antibody A05064, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-16

Question

I see that the anti-ARID2 antibody A05064 works with IHC-P, what is the protocol used to produce the result images on the product page?

R. Li

Verified customer

Asked: 2018-10-17

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-17

Question

Can you help my question with product A05064, anti-ARID2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Taylor

Verified customer

Asked: 2014-03-13

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05064 anti-ARID2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-03-13

Size

Conjugation

Total: $315

SKU:A05064

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
A05064
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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