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- Table of Contents
MCAM antibody (M01683-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat, Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of A375 cells, using CD146 Antibody.
Designing a Western blot for MCAM? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MCAM Western Blot GuideBundle Save
Anti-CD146 MCAM Rabbit Monoclonal Antibody
MCAM antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat, Zebrafish | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
MCAM antibody was positively detected by WB in:
human U2OS whole cell, human K562 whole cell, human Hela whole cell, human A549 whole cell, rat lung tissue, rat spleen tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MCAM antibody was positively detected by IHC in:
human melanoma tissue, human placenta tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MCAM antibody was positively detected by ICC/IF in:
A375 cell
MCAM antibody was positively detected by IF in:
Zebrafish brain
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Zebrafish
Recommend
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CD146 MCAM Rabbit Monoclonal Antibody
View all MCAM Antibodies
View all MCAM Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AbC83
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD146
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated Molecular Weight
71.6 kDa
Cite This Product
Anti-CD146 MCAM Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683-1)
Protocols
Western Blot protocol for MCAM antibody
Immunohistochemistry (IHC) protocol for MCAM antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MCAM antibody
Immunofluorescence (IF) protocol for MCAM antibody
Review
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Product Promise
We promise Anti-CD146 MCAM Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01683-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Nov 1, 2026