Anti-Fibronectin/FN1 Antibody Picoband™

Fibronectin antibody

Boster Bio Anti-Fibronectin/FN1 Antibody Picoband™ catalog # A00564-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 18 publication(s).

Product Info Summary

SKU: A00564-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

Product Name

Anti-Fibronectin/FN1 Antibody Picoband™

View all Fibronectin Antibodies

SKU/Catalog Number

A00564-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fibronectin/FN1 Antibody Picoband™ catalog # A00564-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fibronectin/FN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00564-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Fibronectin/FN1 recombinant protein (Position: T1877-E2386).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00564-1 is reactive to FN1 in Human, Mouse, Rat

Applications

A00564-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Background of Fibronectin

Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For FN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02751

Gene ID

2335

Gene Name

FN1

Full Name

Fibronectin

Weight

272.32kDa

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FN1, check out the FN1 Infographic

FN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00564-1 has been cited in 18 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fibronectin-/fibrinogen-binding protein (FBPS) is not a critical virulence factor for the Streptococcus suis serotype 2 strain ZY05719

Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
Species: Mouse
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:500

Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200

Zhang X,Ke PX,Yuan X,Zhang GP,Chen WL,Zhang GS. Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice. Biomed Res Int.2021 Jan 29;2021:8881843.doi:10.1155/2021/8881843.PM
Species: Mouse
A00564-1 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:NA

Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION1:1000

Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
A00564-1 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:2000

An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
A00564-1 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:200

Li Q,Liu H,Du D,Yu Y,Ma C,Jiao F,Yao H,Lu C,Zhang W. Corrigendum: Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2.Front Cell Infect Microbiol.2020 Oct 29;10:593413.
Species: E.coli, Human
A00564-1 usage in article: APP:WB, SAMPLE:BACTERIAL CULTURE, DILUTION:1:2000

Shi W, Bian L, Lv D, Bi S, Dai Y, Yang K, Lu H, Zhou H, Que Y, Wang D, Zhang Z, Lu N. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids. 2020 Nov 27. doi: 10.1007/
Species: Rat,Adenovirus
A00564-1 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:1:1000

Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in %u2026

Rosiglitazone prevents advanced glycation end products%u2013induced renal toxicity likely through suppression of plasminogen activator inhibitor-1

Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp . zooepidemicus

Effects of tanshinone IIA on the transforming growth factor %u03B21/Smad signaling pathway in rat cardiac fibroblasts

Elongation Factor Thermo Unstable (EF-Tu) Moonlights as an Adhesin on the Surface of Mycoplasma hyopneumoniae by Binding to Fibronectin

Pathophysiology of chronic pancreatitis induced by dibutyltin dichloride joint ethanol in mice

Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...

Wang H, Wang Hs, Zhou Bh, Li Cl, Zhang F, Wang Xf, Zhang G, Bu Xz, Cai Sh, Du J. Plos One. 2013;8(2):E56664. Doi: 10.1371/Journal.Pone.0056664. Epub 2013 Feb 19. Epithelial-Mesenchymal Transition (Emt) Induced By Tnf-?? Requires Akt/Gsk-3??-Mediat...

Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.

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5 Customer Q&As for Anti-Fibronectin/FN1 Antibody Picoband™

Question

We have seen staining in mouse liver. Any tips? Is anti-Fibronectin/FN1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

From literature liver does express FN1. From Uniprot.org, FN1 is expressed in tendon, retinal pigment epithelium, aortic endothelium, amygdala, cervix, colon endothelium, endometrial tumor rc uterine endothelium, mammary cancer, fibroblast, plasma, peripheral blood t-cell umbilical vein endothelial cell, urine, cartilage, mammary carcinoma, cervix carcinoma, liver, among other tissues. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963

Boster Scientific Support

Answered: 2020-02-03

Question

We are currently using anti-Fibronectin/FN1 antibody A00564-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

The anti-Fibronectin/FN1 antibody (A00564-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-27

Question

My colleagues were content with the WB result of your anti-Fibronectin/FN1 antibody. However we have seen positive staining in urine extracellular space using this antibody. Is that expected? Could you tell me where is FN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

From literature, urine does express FN1. Generally FN1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963

Boster Scientific Support

Answered: 2019-11-19

Question

I would like using your anti-Fibronectin/FN1 antibody for extracellular matrix organization studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-05-01

Answer

We appreciate your inquiry. This A00564-1 anti-Fibronectin/FN1 antibody is validated on hela cells. It is guaranteed to work for ELISA, Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-05-01

Question

We ordered your anti-Fibronectin/FN1 antibody for WB on amygdala in the past. I am using rat, and We intend to use the antibody for ELISA next. I am looking for examining amygdala as well as peripheral blood t-cell umbilical vein endothelial cell in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

I took a look at the website and datasheets of our anti-Fibronectin/FN1 antibody and it appears that A00564-1 has been validated on rat in both WB and ELISA. Thus A00564-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-03

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00564-1

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