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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||ELISA, Flow Cytometry, WB|
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Anti-Fibronectin/FN1 Antibody Picoband™
Boster Bio Anti-Fibronectin/FN1 Antibody Picoband™ catalog # A00564-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibronectin/FN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00564-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human Fibronectin/FN1 recombinant protein (Position: T1877-E2386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00564-1 is reactive to FN1 in Human, Mouse, Rat
A00564-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Background of Fibronectin
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of CACO-2 cells using anti-FN1 antibody (A00564-1).
Overlay histogram showing CACO-2 cells stained with A00564-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 2. Flow Cytometry analysis of HeLa cells using anti-FN1 antibody (A00564-1).
Overlay histogram showing HeLa cells stained with A00564-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 3. Western blot analysis of FN1 using anti-FN1 antibody (A00564-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 263KD. The expected band size for FN1 is at 263KD.
Gene/Protein Information For FN1 (Source: Uniprot.Org, NCBI)
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FN1, check out the FN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00564-1 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fibronectin-/fibrinogen-binding protein (FBPS) is not a critical virulence factor for the Streptococcus suis serotype 2 strain ZY05719
Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:500
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Zhang X,Ke PX,Yuan X,Zhang GP,Chen WL,Zhang GS. Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice. Biomed Res Int.2021 Jan 29;2021:8881843.doi:10.1155/2021/8881843.PM
A00564-1 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:NA
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
A00564-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION1:1000
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
A00564-1 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:2000
An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
A00564-1 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:200
Li Q,Liu H,Du D,Yu Y,Ma C,Jiao F,Yao H,Lu C,Zhang W. Corrigendum: Identification of Novel Laminin- and Fibronectin-Binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2.Front Cell Infect Microbiol.2020 Oct 29;10:593413.
Species: E.coli, Human
A00564-1 usage in article: APP:WB, SAMPLE:BACTERIAL CULTURE, DILUTION:1:2000
Shi W, Bian L, Lv D, Bi S, Dai Y, Yang K, Lu H, Zhou H, Que Y, Wang D, Zhang Z, Lu N. Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids. 2020 Nov 27. doi: 10.1007/
A00564-1 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:1:1000
Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in %u2026
Rosiglitazone prevents advanced glycation end products%u2013induced renal toxicity likely through suppression of plasminogen activator inhibitor-1
Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp . zooepidemicus
Effects of tanshinone IIA on the transforming growth factor %u03B21/Smad signaling pathway in rat cardiac fibroblasts
Elongation Factor Thermo Unstable (EF-Tu) Moonlights as an Adhesin on the Surface of Mycoplasma hyopneumoniae by Binding to Fibronectin
Pathophysiology of chronic pancreatitis induced by dibutyltin dichloride joint ethanol in mice
Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...
Wang H, Wang Hs, Zhou Bh, Li Cl, Zhang F, Wang Xf, Zhang G, Bu Xz, Cai Sh, Du J. Plos One. 2013;8(2):E56664. Doi: 10.1371/Journal.Pone.0056664. Epub 2013 Feb 19. Epithelial-Mesenchymal Transition (Emt) Induced By Tnf-?? Requires Akt/Gsk-3??-Mediat...
Li T, Yang Z, Cai Hj, Song Lw, Lu Ky, Zhou Z, Wu Zd. World J Gastroenterol. 2010 Feb 14;16(6):749-54. Effects Of In Vitro Cultivated Calculus Bovis Compound On Pulmonary Lesions In Rabbits With Schistosomiasis.
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