Click image to see more details
Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
Customers Who Bought This Also Bought
Rat Fibronectin ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Fibronectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0350)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: from plasma
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0350 is reactive to Fn1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0350 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fibronectin
Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250KDa.
|EK0350-CAP||Anti-Rat Fn1 Pre-coated 96-well strip microplate||1|
|EK0350-ST||Rat Fn1 Standard||2 vials, 10 ng/tube|
|EK0350-DA||Rat Fn1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Rat Fibronectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Fibronectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Fn1 (Source: Uniprot.Org, NCBI)
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF Fn1|FIBNEC, fn-1|fibronectin 1|fibronectin|FN|fibronectin 2|fibronectin 3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Fn1, check out the Fn1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0350 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase
PERK-Dependent Activation of the JAK2/STAT3 Pathway Contributes to High Glucose-Induced Extracellular Matrix Deposition in Renal Tubular Epithelial Cells
Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways
Preservation of osteoblasts and BM-MSCs biological properties after consecutive passages with the thermal-liftoff method
Artesunate ameliorates high glucose-induced rat glomerular mesangial cell injury by suppressing the TLR4/NF-κB/NLRP3 inflammasome pathway
Metformin alleviates bleomycin-induced pulmonary fibrosis in rats: Pharmacological effects and molecular mechanisms
Yu,X.,Su,Q.,Geng,J.,Liu,H.,Liu,Y.,Liu,J. ... Zou,Y.(2021).Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase.Experimental and Therapeutic Medicine,21,333.https://doi.org/10.3892/etm.2021.97
EK0350 usage in article: APP:ELISA, SAMPLE:HBZY-1 CELL, DILUTION:NA
Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy
The Ayurvedic Medicine
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...
Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.
Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.
Mao T, Gao L, Li H, Li J. Saudi Med J. 2011 Aug;32(8):769-77. Pigment Epithelium-Derived Factor Inhibits High Glucose Induced Oxidative Stress And Fibrosis Of Cultured Human Glomerular Mesangial Cells.
Yang L, Cheng F, Liu T, Lu Jr, Song K, Jiang L, Wu S, Guo W. Biomed Mater. 2012 Jun;7(3):035003. Doi: 10.1088/1748-6041/7/3/035003. Epub 2012 Feb 23. Comparison Of Mesenchymal Stem Cells Released From Poly(N-Isopropylacrylamide) Copolymer Film And...
Tang Kf, Song Gb, Shi Ys, Yuan L, Li Yh. Biochim Biophys Acta. 2010 Mar;1800(3):380-4. Doi: 10.1016/J.Bbagen.2009.11.009. Epub 2009 Nov 13. Dicer Knockdown Induces Fibronectin-1 Expression In Hek293T Cells Via Induction Of Egr1.
Zeng R, Han M, Luo Y, Li C, Pei G, Liao W, Bai S, Ge S, Liu X, Xu G. Nephrol Dial Transplant. 2011 Apr;26(4):1149-56. Doi: 10.1093/Ndt/Gfq619. Epub 2010 Oct 19. Role Of Sema4C In Tgf-??1-Induced Mitogen-Activated Protein Kinase Activation And Epit...
Bai S, Zeng R, Zhou Q, Liao W, Zhang Y, Xu C, Han M, Pei G, Liu L, Liu X, Yao Y, Xu G. Int J Biol Sci. 2012;8(6):859-69. Doi: 10.7150/Ijbs.3490. Epub 2012 Jun 13. Cdc42-Interacting Protein-4 Promotes Tgf-??1-Induced Epithelial-Mesenchymal Transiti...
Xu Ww, Guan Mp, Zheng Zj, Gao F, Zeng Ym, Qin Y, Xue Ym. Cell Physiol Biochem. 2014;33(2):423-32. Doi: 10.1159/000358623. Epub 2014 Feb 11. Exendin-4 Alleviates High Glucose-Induced Rat Mesangial Cell Dysfunction Through The Ampk Pathway.
Zirkel A, Lederer M, St??hr N, Pazaitis N, H??ttelmaier S. Nucleic Acids Res. 2013 Jul;41(13):6618-36. Doi: 10.1093/Nar/Gkt410. Epub 2013 May 15. Igf2Bp1 Promotes Mesenchymal Cell Properties And Migration Of Tumor-Derived Cells By Enhancing The Ex...
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Rat Fibronectin ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Rat Fibronectin ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question