Rat Fibronectin ELISA Kit PicoKine®

Fibronectin ELISA kit for Rat

Rat Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. Cited in 19 publication(s).

Product Info Summary

SKU: EK0350
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Rat Fibronectin ELISA Kit PicoKine®

View all Fibronectin ELISA kits

SKU/Catalog Number

EK0350

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat Fibronectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0350)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: from plasma

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0350 is reactive to Fn1 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0350 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Fibronectin

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from rat plasma with the molecular mass of 200-250KDa.

Kit Components

Catalog Number Description Quantity
EK0350-CAP Anti-Rat Fn1 Pre-coated 96-well strip microplate 1
EK0350-ST Rat Fn1 Standard 2 vials, 10 ng/tube
EK0350-DA Rat Fn1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631362512502500500010000
O.D.0.0120.0520.1110.2080.3810.6681.2011.952

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat Fibronectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3421852432636118344010
Standard deviation23.2585.49190.3429.96102.7216.54
CV (%)6.8%4.6%4.4%8.3%5.6%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13423223423453379.172.7%
Sample 21852188217802001187879.724.2%
Sample 343264126377240984080198.634.8%
*number of samples for each test n=16.

Gene/Protein Information For Fn1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04937

Gene ID

25661

Gene Name

Fn1

Full Name

Fibronectin

Weight

272.511kDa

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF Fn1|FIBNEC, fn-1|fibronectin 1|fibronectin|FN|fibronectin 2|fibronectin 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fn1, check out the Fn1 Infographic

Fn1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0350 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase

PERK-Dependent Activation of the JAK2/STAT3 Pathway Contributes to High Glucose-Induced Extracellular Matrix Deposition in Renal Tubular Epithelial Cells

Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways

Preservation of osteoblasts and BM-MSCs biological properties after consecutive passages with the thermal-liftoff method

Artesunate ameliorates high glucose-induced rat glomerular mesangial cell injury by suppressing the TLR4/NF-κB/NLRP3 inflammasome pathway

Metformin alleviates bleomycin-induced pulmonary fibrosis in rats: Pharmacological effects and molecular mechanisms

Yu,X.,Su,Q.,Geng,J.,Liu,H.,Liu,Y.,Liu,J. ... Zou,Y.(2021).Ginkgo biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase.Experimental and Therapeutic Medicine,21,333.https://doi.org/10.3892/etm.2021.97
Species: Rat
EK0350 usage in article: APP:ELISA, SAMPLE:HBZY-1 CELL, DILUTION:NA

Effect of Wujia Shenghua capsule on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy

The Ayurvedic Medicine

Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

Mao T, Gao L, Li H, Li J. Saudi Med J. 2011 Aug;32(8):769-77. Pigment Epithelium-Derived Factor Inhibits High Glucose Induced Oxidative Stress And Fibrosis Of Cultured Human Glomerular Mesangial Cells.

Yang L, Cheng F, Liu T, Lu Jr, Song K, Jiang L, Wu S, Guo W. Biomed Mater. 2012 Jun;7(3):035003. Doi: 10.1088/1748-6041/7/3/035003. Epub 2012 Feb 23. Comparison Of Mesenchymal Stem Cells Released From Poly(N-Isopropylacrylamide) Copolymer Film And...

Tang Kf, Song Gb, Shi Ys, Yuan L, Li Yh. Biochim Biophys Acta. 2010 Mar;1800(3):380-4. Doi: 10.1016/J.Bbagen.2009.11.009. Epub 2009 Nov 13. Dicer Knockdown Induces Fibronectin-1 Expression In Hek293T Cells Via Induction Of Egr1.

Zeng R, Han M, Luo Y, Li C, Pei G, Liao W, Bai S, Ge S, Liu X, Xu G. Nephrol Dial Transplant. 2011 Apr;26(4):1149-56. Doi: 10.1093/Ndt/Gfq619. Epub 2010 Oct 19. Role Of Sema4C In Tgf-??1-Induced Mitogen-Activated Protein Kinase Activation And Epit...

Bai S, Zeng R, Zhou Q, Liao W, Zhang Y, Xu C, Han M, Pei G, Liu L, Liu X, Yao Y, Xu G. Int J Biol Sci. 2012;8(6):859-69. Doi: 10.7150/Ijbs.3490. Epub 2012 Jun 13. Cdc42-Interacting Protein-4 Promotes Tgf-??1-Induced Epithelial-Mesenchymal Transiti...

Xu Ww, Guan Mp, Zheng Zj, Gao F, Zeng Ym, Qin Y, Xue Ym. Cell Physiol Biochem. 2014;33(2):423-32. Doi: 10.1159/000358623. Epub 2014 Feb 11. Exendin-4 Alleviates High Glucose-Induced Rat Mesangial Cell Dysfunction Through The Ampk Pathway.

Zirkel A, Lederer M, St??hr N, Pazaitis N, H??ttelmaier S. Nucleic Acids Res. 2013 Jul;41(13):6618-36. Doi: 10.1093/Nar/Gkt410. Epub 2013 May 15. Igf2Bp1 Promotes Mesenchymal Cell Properties And Migration Of Tumor-Derived Cells By Enhancing The Ex...

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5 Customer Q&As for Rat Fibronectin ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

E. Miller

Verified customer

Asked: 2020-12-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-02

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Fibronectin in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

D. Thomas

Verified customer

Asked: 2020-07-04

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Fibronectin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-07-04

Question

Q: how do I analyze ELISA data? I have obtained Fibronectin level in serum.

Verified Customer

Verified customer

Asked: 2019-09-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-01

Question

Q: What is the optimal O.D. value for Fibronectin ELISA kit? I used your Fibronectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Fibronectin even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-01-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Fibronectin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Fibronectin that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2019-01-27

Question

Q: is it okay to use heparin plasma as samples in Rat Fibronectin Picokine® ELISA Kit (Catalog # EK0350)?

Verified Customer

Verified customer

Asked: 2018-11-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Fibronectin causing degradation of its protein structure. Fibronectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Fibronectin ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-11-20

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