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Product Info Summary
| SKU: | A09845 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD163b CD163L1 Antibody
SKU/Catalog Number
A09845
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD163b CD163L1 Antibody catalog # A09845. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD163b CD163L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09845)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD163L1. AA range:391-440
Reactive Species
A09845 is reactive to CD163L1 in Human
Calculated molecular weight
159.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09845 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
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Western Blot analysis of HeLa cells using CD163b Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from HeLa cells, using CD163L1 Antibody.
Specific Publications For Anti-CD163b CD163L1 Antibody (A09845)
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Customer Q&As
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3 Customer Q&As for Anti-CD163b CD163L1 Antibody
Question
We are currently using anti-CD163b antibody A09845 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
S. Baker
Verified customer
Asked: 2020-02-13
Answer
The anti-CD163b antibody (A09845) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
Will A09845 anti-CD163b antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
As indicated on the product datasheet, A09845 anti-CD163b antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-17
Question
Is a blocking peptide available for product anti-CD163b antibody (A09845)?
Z. Lewis
Verified customer
Asked: 2014-07-29
Answer
We do provide the blocking peptide for product anti-CD163b antibody (A09845). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-07-29
