Click image to see more details
Product Info Summary
| SKU: | A02158-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD1B Antibody Picoband®
SKU/Catalog Number
A02158-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD1B Antibody Picoband® catalog # A02158-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02158-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD1B recombinant protein (Position: D78-Y110).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02158-2 is reactive to CD1B in Human, Mouse, Rat
Observed Molecular Weight
40 kDa
Calculated molecular weight
36.9 kDa
Background of CD1B
CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02158-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HCCT tissue, human MOLT-4 tissue, human Jurkat whole cell, rat thymus tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse Ana-1 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD1B using anti-CD1B antibody (A02158-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCT tissue lysates,
Lane 2: human MOLT-4 tissue lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (Catalog # A02158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.
Click image to see more details
(A–F) Cisatracurium inhibits metastatic ability of CRC in vivo . (A) Line graph of subcutaneous tumor volume. (B) Weight of subcutaneous tumors in grams. Data are expressed as mean tumor volume or weight ± SE. ∗ p < 0.05. (C–F) Representative densities of tumor viability and migration regulatory proteins (p53, p21 and CD1, SNAI-1, CALD1, E-Cadherin) in tumor tissue samples. β-Actin was used as internal control. The cluster bar chats in (D , F) indicates the levels of viability and migration regulatory proteins in the treatment group. Data are expressed as Mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus control.
Index in PubMed under a CC BY license. PMID: 30108509
Specific Publications For Anti-CD1B Antibody Picoband® (A02158-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD1B Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD1B Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
