Anti-B-cell receptor CD22 CD22 Antibody

Boster Bio Anti-B-cell receptor CD22 CD22 Antibody catalog # PA1018-1. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PA1018-1
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-B-cell receptor CD22 CD22 Antibody

See all Siglec-2/CD22 products

SKU/Catalog Number

PA1018-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-B-cell receptor CD22 CD22 Antibody catalog # PA1018-1. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-B-cell receptor CD22 CD22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1018-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (832-847aa ERPQAQENVDYVILKH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1018-1 is reactive to CD22 in Human

Applications

PA1018-1 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD22 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20273

Gene ID

933

Gene Name

CD22

Full Name

B-cell receptor CD22

Weight

95.348kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD22, check out the CD22 Infographic

CD22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1018-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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17 Customer Q&As for Anti-B-cell receptor CD22 CD22 Antibody

Question

Do you have a BSA free version of anti-CD22 antibody PA1018-1 available?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD22 antibody PA1018-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-02

Question

See attached the WB image, lot number and protocol we used for tonsil using anti-CD22 antibody PA1018-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-12

Question

Can you help my question with product PA1018-1, anti-CD22 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1018-1 anti-CD22 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-11

Question

We ordered your anti-CD22 antibody for IHC on tonsil in the past. I am using human, and We intend to use the antibody for WB next. I am interested in examining tonsil as well as corpus callosum in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

I have checked the website and datasheets of our anti-CD22 antibody and it appears that PA1018-1 has been tested on human in both IHC and WB. Thus PA1018-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-11

Question

I was wanting to use your anti-CD22 antibody for WB for human tonsil on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tonsil identification?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

It shows on the product datasheet, PA1018-1 anti-CD22 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tonsil in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-04

Question

We have seen staining in human corpus callosum. Are there any suggestions? Is anti-CD22 antibody supposed to stain corpus callosum positively?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

Based on literature corpus callosum does express CD22. Based on Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2019-06-18

Question

We are currently using anti-CD22 antibody PA1018-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The anti-CD22 antibody (PA1018-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-03

Question

Is a blocking peptide available for product anti-CD22 antibody (PA1018-1)?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

We do provide the blocking peptide for product anti-CD22 antibody (PA1018-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-03-22

Question

Will PA1018-1 anti-CD22 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-19

Answer

You can see on the product datasheet, PA1018-1 anti-CD22 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-19

Question

We need to test anti-CD22 antibody PA1018-1 on human tonsil for research purposes, then I may be interested in using anti-CD22 antibody PA1018-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-09-06

Answer

The products we sell, including anti-CD22 antibody PA1018-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-09-06

Question

I see that the anti-CD22 antibody PA1018-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-30

Question

I was wanting to use using your anti-CD22 antibody for regulation of b cell proliferation studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.

K. Evans

Verified customer

Asked: 2018-05-04

Answer

We appreciate your inquiry. This PA1018-1 anti-CD22 antibody is tested on raji cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-05-04

Question

Is this PA1018-1 anti-CD22 antibody reactive to the isotypes of CD22?

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

The immunogen of PA1018-1 anti-CD22 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD22(832-847aa ERPQAQENVDYVILKH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-11

Question

Will anti-CD22 antibody PA1018-1 work for WB with tonsil?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

According to the expression profile of tonsil, CD22 is highly expressed in tonsil. So, it is likely that anti-CD22 antibody PA1018-1 will work for WB with tonsil.

Boster Scientific Support

Answered: 2017-08-15

Question

Will anti-CD22 antibody PA1018-1 work on canine WB with tonsil?

K. Miller

Verified customer

Asked: 2017-03-28

Answer

Our lab technicians have not validated anti-CD22 antibody PA1018-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CD22 antibody PA1018-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine tonsil in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-03-28

Question

My team were content with the WB result of your anti-CD22 antibody. However we have observed positive staining in corpus callosum cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?

V. Jones

Verified customer

Asked: 2015-11-04

Answer

From what I have seen in literature, corpus callosum does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2015-11-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tonsil using anti-CD22 antibody PA1018-1. Let me know if you need anything else.

W. Li

Verified customer

Asked: 2014-12-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-12-10

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PA1018-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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