Anti-CD22 Antibody Picoband™

Siglec-2/CD22 antibody

Boster Bio Anti-CD22 Antibody Picoband™ catalog # PB9691. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PB9691
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-CD22 Antibody Picoband™

View all Siglec-2/CD22 Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-CD22 Antibody Picoband™ catalog # PB9691. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD22 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9691)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.




Rabbit IgG


A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9691 is reactive to CD22 in Human, Mouse, Rat


PB9691 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Background of Siglec-2/CD22

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD22 (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

B-cell receptor CD22




immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD22, check out the CD22 Infographic

CD22 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9691 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yu X, Li L, Li Q, Zang X, Liu Z. Biol Trace Elem Res. 2011 Nov;143(2):1064-76. Doi: 10.1007/S12011-010-8941-5. Epub 2011 Jan 12. Trail And Dr5 Promote Thyroid Follicular Cell Apoptosis In Iodine Excess-Induced Experimental Autoimmune Thyroiditis I...

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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5 Customer Q&As for Anti-CD22 Antibody Picoband™


I am interested in using your anti-CD22 antibody for regulation of endocytosis studies. Has this antibody been tested with western blotting on raji cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-22


Thanks for your inquiry. This PB9691 anti-CD22 antibody is tested on human tonsil tissue, raji cells, rat thymus tissue, mouse thymus tissue. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-22


We are currently using anti-CD22 antibody PB9691 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-30


The anti-CD22 antibody (PB9691) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-30


Our lab were content with the WB result of your anti-CD22 antibody. However we have observed positive staining in tonsil cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-11


From what I have seen in literature, tonsil does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2019-11-11


We ordered your anti-CD22 antibody for WB on tonsil last year. I am using human, and We intend to use the antibody for IHC-P next. I am interested in examining tonsil as well as corpus callosum in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2018-12-18


I viewed the website and datasheets of our anti-CD22 antibody and it seems that PB9691 has been tested on human in both WB and IHC-P. Thus PB9691 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website to find out more information about them.

Boster Scientific Support

Answered: 2018-12-18


We have seen staining in mouse tonsil. Are there any suggestions? Is anti-CD22 antibody supposed to stain tonsil positively?

Verified Customer

Verified customer

Asked: 2018-07-11


Based on literature tonsil does express CD22. Based on, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2018-07-11



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