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Product Info Summary
| SKU: | PB9691 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD22 Antibody Picoband®
SKU/Catalog Number
PB9691
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD22 Antibody Picoband® catalog # PB9691. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD22 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9691)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD22, different from the related mouse sequence by ten amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9691 is reactive to CD22 in Human, Mouse, Rat
Observed Molecular Weight
140 kDa
Calculated molecular weight
95.3 kDa
Background of CD22
CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9691 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106cells, Human
Positive Control
WB: human Daudi whole cell, human Raji whole cell, human Ramos whole cell
IHC: human tonsil tissue
FCM: Raji cell
Validation Images & Assay Conditions
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IHC analysis of CD22 using anti-CD22 antibody (PB9691).
CD22 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD22 Antibody (PB9691) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of CD22 using anti-CD22 antibody (PB9691).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD22 antigen affinity purified polyclonal antibody (Catalog # PB9691) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140kDa. The expected band size for CD22 is at 95 kDa.
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Flow Cytometry analysis of Raji cells using anti-CD22 antibody (PB9691).
Overlay histogram showing Raji cells stained with PB9691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (PB9691,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-CD22 Antibody Picoband® (PB9691)
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5 Customer Q&As for Anti-CD22 Antibody Picoband®
Question
I am interested in using your anti-CD22 antibody for regulation of endocytosis studies. Has this antibody been tested with western blotting on raji cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Thanks for your inquiry. This PB9691 anti-CD22 antibody is tested on human tonsil tissue, raji cells, rat thymus tissue, mouse thymus tissue. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-CD22 antibody PB9691 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The anti-CD22 antibody (PB9691) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-30
Question
Our lab were content with the WB result of your anti-CD22 antibody. However we have observed positive staining in tonsil cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
From what I have seen in literature, tonsil does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2019-11-11
Question
We ordered your anti-CD22 antibody for WB on tonsil last year. I am using human, and We intend to use the antibody for IHC-P next. I am interested in examining tonsil as well as corpus callosum in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
I viewed the website and datasheets of our anti-CD22 antibody and it seems that PB9691 has been tested on human in both WB and IHC-P. Thus PB9691 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-18
Question
We have seen staining in mouse tonsil. Are there any suggestions? Is anti-CD22 antibody supposed to stain tonsil positively?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
Based on literature tonsil does express CD22. Based on Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2018-07-11
