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Product Info Summary
| SKU: | M01572-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-CD22 Antibody Picoband® (monoclonal, 5E7)
SKU/Catalog Number
M01572-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD22 Antibody Picoband® (monoclonal, 5E7) catalog # M01572-2. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD22 Antibody Picoband® (monoclonal, 5E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01572-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
5E7
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD22, different from the related mouse sequence by ten amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01572-2 is reactive to CD22 in Human
Observed Molecular Weight
140 kDa
Calculated molecular weight
95.3 kDa
Background of CD22
CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01572-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Raji tissue,
Validation Images & Assay Conditions
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Western blot analysis of CD22 using anti ZO-1 antibody (M01572-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD22 antigen affinity purified polyclonal antibody (Catalog # M01572-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.
Specific Publications For Anti-CD22 Antibody Picoband® (monoclonal, 5E7) (M01572-2)
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4 Customer Q&As for Anti-CD22 Antibody Picoband® (monoclonal, 5E7)
Question
My colleagues were happy with the WB result of your anti-CD22 antibody (monoclonal, 5E7). However we have observed positive staining in corpus callosum cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
Based on literature, corpus callosum does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2020-01-28
Question
We have seen staining in human tonsil. Are there any suggestions? Is anti-CD22 antibody (monoclonal, 5E7) supposed to stain tonsil positively?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to literature tonsil does express CD22. According to Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119
Boster Scientific Support
Answered: 2020-01-15
Question
We ordered your anti-CD22 antibody (monoclonal, 5E7) for Flow Cytometry on spleen in the past. I am using human, and We are going to use the antibody for WB next. My question regards examining spleen as well as tonsil in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
I took a look at the website and datasheets of our anti-CD22 antibody (monoclonal, 5E7) and it seems that M01572-2 has been validated on human in both Flow Cytometry and WB. Thus M01572-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-CD22 antibody (monoclonal, 5E7) M01572-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
The anti-CD22 antibody (monoclonal, 5E7) (M01572-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-06
