Anti-CD22 Antibody Picoband™ (monoclonal, 5E7)

Siglec-2/CD22 antibody

Boster Bio Anti-CD22 Antibody Picoband™ (monoclonal, 5E7) catalog # M01572-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M01572-2
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, WB

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Product Name

Anti-CD22 Antibody Picoband™ (monoclonal, 5E7)

View all Siglec-2/CD22 Antibodies

SKU/Catalog Number

M01572-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD22 Antibody Picoband™ (monoclonal, 5E7) catalog # M01572-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD22 Antibody Picoband™ (monoclonal, 5E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01572-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

5E7

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01572-2 is reactive to CD22 in Human

Applications

M01572-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Background of Siglec-2/CD22

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD22 (Source: Uniprot.org, NCBI)

Uniprot ID

P20273

Gene ID

933

Gene Name

CD22

Full Name

B-cell receptor CD22

Weight

95.348kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD22, check out the CD22 Infographic

CD22 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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4 Customer Q&As for Anti-CD22 Antibody Picoband™ (monoclonal, 5E7)

Question

My colleagues were happy with the WB result of your anti-CD22 antibody (monoclonal, 5E7). However we have observed positive staining in corpus callosum cell membrane using this antibody. Is that expected? Could you tell me where is CD22 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

Based on literature, corpus callosum does express CD22. Generally CD22 expresses in cell membrane. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2020-01-28

Question

We have seen staining in human tonsil. Are there any suggestions? Is anti-CD22 antibody (monoclonal, 5E7) supposed to stain tonsil positively?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

According to literature tonsil does express CD22. According to Uniprot.org, CD22 is expressed in corpus callosum, tonsil, spleen, among other tissues. Regarding which tissues have CD22 expression, here are a few articles citing expression in various tissues:
Spleen, Pubmed ID: 15057824
Tonsil, Pubmed ID: 1985119

Boster Scientific Support

Answered: 2020-01-15

Question

We ordered your anti-CD22 antibody (monoclonal, 5E7) for Flow Cytometry on spleen in the past. I am using human, and We are going to use the antibody for WB next. My question regards examining spleen as well as tonsil in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

I took a look at the website and datasheets of our anti-CD22 antibody (monoclonal, 5E7) and it seems that M01572-2 has been validated on human in both Flow Cytometry and WB. Thus M01572-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-03

Question

We are currently using anti-CD22 antibody (monoclonal, 5E7) M01572-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-06

Answer

The anti-CD22 antibody (monoclonal, 5E7) (M01572-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-06

Size

Conjugation

Total: $315

SKU:M01572-2

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M01572-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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