Anti-Cd27 Antibody Picoband™

Product Name

Anti-Cd27 Antibody Picoband™

View all CD27/TNFRSF7 Antibodies

SKU/Catalog Number

A01148-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cd27 Antibody Picoband™ catalog # A01148-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cd27 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01148-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cd27 recombinant protein (Position: P24-F187).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01148-2 is reactive to CD27 in Mouse, Rat

Applications

A01148-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

29.137kDa

Background of CD27/TNFRSF7

CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 2. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details

Figure 3. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details

Figure 4. Flow Cytometry analysis of mouse PBMC cells using anti-Cd27 antibody (A01148-2).
Overlay histogram showing mouse PBMC cells stained with A01148-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd27 Antibody (A01148-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CD27 (Source: Uniprot.org, NCBI)

Alternative Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 antigen|CD27L receptor|T cell activation antigen S152|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily, member 7

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