Anti-ABCG8 Antibody Picoband™

ABCG8 antibody

Boster Bio Anti-ABCG8 Antibody Picoband™ catalog # A01482-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01482-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, ICC, WB

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Product Name

Anti-ABCG8 Antibody Picoband™

View all ABCG8 Antibodies

SKU/Catalog Number

A01482-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCG8 Antibody Picoband™ catalog # A01482-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCG8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01482-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ABCG8, different from the related mouse and rat sequences by twelve amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01482-1 is reactive to ABCG8 in Human, Mouse, Rat

Applications

A01482-1 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

75.679kDa

Background of ABCG8

ATP-binding cassette sub-family G member 8 is a protein that in humans is encoded by the ABCG8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ABCG8 (Source: Uniprot.org, NCBI)

Gene Name

ABCG8

Full Name

ATP-binding cassette sub-family G member 8

Weight

75.679kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2); ATP-binding cassette, sub-family G (WHITE), member 8; GBD4ATP-binding cassette, subfamily G, member 8; MGC142217; sterolin 2; sterolin-2; STSL ABCG8 GBD4, STSL, STSL1 ATP binding cassette subfamily G member 8 ATP-binding cassette sub-family G member 8|ATP-binding cassette, sub-family G (WHITE), member 8|sterolin 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABCG8, check out the ABCG8 Infographic

ABCG8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABCG8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-ABCG8 Antibody Picoband™

Question

I have attached the WB image, lot number and protocol we used for liver using anti-ABCG8 antibody A01482-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-10

Question

Our lab want to know about to test anti-ABCG8 antibody A01482-1 on human liver for research purposes, then I may be interested in using anti-ABCG8 antibody A01482-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The products we sell, including anti-ABCG8 antibody A01482-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-14

Question

I have a question about product A01482-1, anti-ABCG8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01482-1 anti-ABCG8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-10

Question

Will anti-ABCG8 antibody A01482-1 work on pig WB with right lobe of liver?

Verified Customer

Verified customer

Asked: 2018-12-25

Answer

Our lab technicians have not validated anti-ABCG8 antibody A01482-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABCG8 antibody A01482-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig right lobe of liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-25

Question

Is a blocking peptide available for product anti-ABCG8 antibody (A01482-1)?

Verified Customer

Verified customer

Asked: 2018-11-26

Answer

We do provide the blocking peptide for product anti-ABCG8 antibody (A01482-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-26

Size

Conjugation

Total: $370

SKU:A01482-1

In stock, 1 left.

Order within 1 hours and 32 minutes to receive by Wed Mar 20

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A01482-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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