Anti-ABCG8 Antibody

ABCG8 antibody

Boster Bio Anti-ABCG8 Antibody catalog # A01482-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01482-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, ICC, WB

Product Name

Anti-ABCG8 Antibody

View all ABCG8 Antibodies

SKU/Catalog Number

A01482-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCG8 Antibody catalog # A01482-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCG8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01482-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ABCG8 recombinant protein (Position: R50-D672).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01482-2 is reactive to ABCG8 in Human, Mouse, Rat

Applications

A01482-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

75.679kDa

Background of ABCG8

ATP-binding cassette sub-family G member 8 is a protein that in humans is encoded by the ABCG8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ABCG8 (Source: Uniprot.org, NCBI)

Gene Name

ABCG8

Full Name

ATP-binding cassette sub-family G member 8

Weight

75.679kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2); ATP-binding cassette, sub-family G (WHITE), member 8; GBD4ATP-binding cassette, subfamily G, member 8; MGC142217; sterolin 2; sterolin-2; STSL ABCG8 GBD4, STSL, STSL1 ATP binding cassette subfamily G member 8 ATP-binding cassette sub-family G member 8|ATP-binding cassette, sub-family G (WHITE), member 8|sterolin 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABCG8, check out the ABCG8 Infographic

ABCG8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABCG8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01482-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang J,Wang Q,Chen M,Bi Y,Shi H,Zhou K. Effects of psoralen on hepatic bile acid transporters in rats. Hum Exp Toxicol.2020 Dec 15:960327120979346.doi:10.1177/0960327120979346. Epub ahead of print.PMID:33317360.
Species: Rat
A01482-2 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500

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1 Customer Q&As for Anti-ABCG8 Antibody

Question

We are currently using anti-ABCG8 antibody A01482-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

The anti-ABCG8 antibody (A01482-2) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-13

Size

Conjugation

Total: $370

SKU:A01482-2

In stock, 1 left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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