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Product Info Summary
| SKU: | M02220-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335
SKU/Catalog Number
M02220-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (Catalog # M02220-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM335
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD276 (B7-H3).
Cross-reactivity
This antibody reacts to the cytoplasmic domain of human CD276 (B7-H3).
Reactive Species
M02220-1 is reactive to CD276 in Human
Calculated molecular weight
57.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02220-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:5000-1:10,000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of LNCaP cell lysate using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:10,000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:1000 dilution.
Specific Publications For Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 (M02220-1)
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Customer Q&As
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1 Customer Q&As for Anti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335
Question
We are currently using anti-CD276 (B7-H3) Rabbit Monoclonal antibody, Clone#RM335 M02220-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-CD276 (B7-H3) Rabbit Monoclonal antibody, Clone#RM335 (M02220-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
