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Product Info Summary
| SKU: | A02765-2 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CD3 epsilon/Cd3e Antibody Picoband™
SKU/Catalog Number
A02765-2
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD3 epsilon/Cd3e Antibody Picoband™ catalog # A02765-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3 epsilon/Cd3e Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02765-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
E.coli-derived rat CD3 epsilon/Cd3e recombinant protein (Position: Q21-S108).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02765-2 is reactive to Cd3e in Rat
Applications
A02765-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Background of CD3E
CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
"Western blot, 0.1-0.25μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat
"
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cd3e using anti-Cd3e antibody (A02765-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd3e antigen affinity purified polyclonal antibody (Catalog # A02765-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd3e at approximately 23KD. The expected band size for Cd3e is at 23KD.
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Figure 2. IHC analysis of Cd3e using anti-Cd3e antibody (A02765-2).
Cd3e was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cd3e Antibody (A02765-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Cd3e using anti-Cd3e antibody (A02765-2).
Cd3e was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cd3e Antibody (A02765-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Cd3e (Source: Uniprot.Org, NCBI)
Uniprot ID
P07766
Gene ID
916
Gene Name
Cd3e
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
23.147kDa
Alternative Names
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd3e, check out the Cd3e Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd3e: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-CD3 epsilon/Cd3e Antibody Picoband™ (A02765-2)
No publications found for A02765-2
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD3 epsilon/Cd3e Antibody Picoband™
Question
My boss were happy with the WB result of your anti-CD3 epsilon/Cd3e antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
From literature, leukocyte does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-01
Question
We ordered your anti-CD3 epsilon/Cd3e antibody for ELISA on blood in the past. I am using rat, and I plan to use the antibody for WB next. My lab would like examining blood as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
I have checked the website and datasheets of our anti-CD3 epsilon/Cd3e antibody and it seems that A02765-2 has been tested on rat in both ELISA and WB. Thus A02765-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-21
Question
We have been able to see staining in rat thymus. Do you have any suggestions? Is anti-CD3 epsilon/Cd3e antibody supposed to stain thymus positively?
F. Patel
Verified customer
Asked: 2019-01-22
Answer
According to literature thymus does express CD3E. According to Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-01-22
Question
We are currently using anti-CD3 epsilon/Cd3e antibody A02765-2 for rat tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The anti-CD3 epsilon/Cd3e antibody (A02765-2) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-06
Question
We are interested in using your anti-CD3 epsilon/Cd3e antibody for positive thymic t cell selection studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Thanks for your inquiry. This A02765-2 anti-CD3 epsilon/Cd3e antibody is tested on rat thymus tissue, spleen tissue, lymphaden tissue. It is guaranteed to work for ELISA, IHC-P, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-13
