Click image to see more details
Product Info Summary
| SKU: | A02675 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD3 epsilon/Cd3e Antibody Picoband™
SKU/Catalog Number
A02675
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD3 epsilon/Cd3e Antibody Picoband™ catalog # A02675. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD3 epsilon/Cd3e Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02675)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
E. coli-derived mouse CD3 epsilon recombinant protein (Position: D22-D108).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins.
Reactive Species
A02675 is reactive to Cd3e in Mouse, Rat
Applications
A02675 is guaranteed for ELISA, WB Boster Guarantee
Background of Cd3e
CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD3 epsilon using anti-CD3 epsilon antibody (A02675).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon antigen affinity purified polyclonal antibody (Catalog # A02675) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 epsilon at approximately 23KD. The expected band size for CD3 epsilon is at 23KD.
Protein Target Info & Infographic
Gene/Protein Information For Cd3e (Source: Uniprot.Org, NCBI)
Uniprot ID
P22646
Gene ID
12501
Gene Name
Cd3e
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
21.393kDa
Alternative Names
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain Cd3e|AI504783, CD3, CD3epsilon, T3e|CD3 antigen, epsilon polypeptide|T-cell surface glycoprotein CD3 epsilon chain|T-cell surface antigen T3/Leu-4 epsilon chain
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd3e, check out the Cd3e Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd3e: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-CD3 epsilon/Cd3e Antibody Picoband™ (A02675)
A02675 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-CD3 epsilon/Cd3e Antibody Picoband™
Question
We have seen staining in mouse blood. Do you have any suggestions? Is anti-CD3 epsilon/Cd3e antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
From literature blood does express CD3E. From Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-28
Question
Does anti-CD3 epsilon/Cd3e antibody A02675 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to the expression profile of leukemic t-cell, CD3E is highly expressed in leukemic t-cell. So, it is likely that anti-CD3 epsilon/Cd3e antibody A02675 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2020-02-27
Question
We are currently using anti-CD3 epsilon/Cd3e antibody A02675 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The anti-CD3 epsilon/Cd3e antibody (A02675) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-03
Question
I see that the anti-CD3 epsilon/Cd3e antibody A02675 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-27
Question
We were happy with the WB result of your anti-CD3 epsilon/Cd3e antibody. However we have been able to see positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
According to literature, leukemic t-cell does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-26
Question
I would like to test anti-CD3 epsilon/Cd3e antibody A02675 on rat leukemic t-cell for research purposes, then I may be interested in using anti-CD3 epsilon/Cd3e antibody A02675 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The products we sell, including anti-CD3 epsilon/Cd3e antibody A02675, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-05
Question
Is this A02675 anti-CD3 epsilon/Cd3e antibody reactive to the isotypes of CD3E?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The immunogen of A02675 anti-CD3 epsilon/Cd3e antibody is E. coli-derived mouse CD3 epsilon recombinant protein (Position: D22-D108). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-29
Question
Can you help my question with product A02675, anti-CD3 epsilon/Cd3e antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02675 anti-CD3 epsilon/Cd3e antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
Our lab used your anti-CD3 epsilon/Cd3e antibody for ELISA on blood in a previous project. I am using mouse, and We intend to use the antibody for WB next. I would like examining blood as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
S. Walker
Verified customer
Asked: 2019-07-05
Answer
I viewed the website and datasheets of our anti-CD3 epsilon/Cd3e antibody and I see that A02675 has been validated on mouse in both ELISA and WB. Thus A02675 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-05
Question
I was wanting to use your anti-CD3 epsilon/Cd3e antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, A02675 anti-CD3 epsilon/Cd3e antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
We want using your anti-CD3 epsilon/Cd3e antibody for r-mmu-202433; generation of second messenger molecules studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
We appreciate your inquiry. This A02675 anti-CD3 epsilon/Cd3e antibody is validated on rat thymus tissue, tissue lysate. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-28
Question
Would A02675 anti-CD3 epsilon/Cd3e antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
You can see on the product datasheet, A02675 anti-CD3 epsilon/Cd3e antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-01
Question
Is a blocking peptide available for product anti-CD3 epsilon/Cd3e antibody (A02675)?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
We do provide the blocking peptide for product anti-CD3 epsilon/Cd3e antibody (A02675). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 epsilon/Cd3e antibody A02675. Let me know if you need anything else.
L. Zhao
Verified customer
Asked: 2015-06-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-26
Question
Is there a BSA free version of anti-CD3 epsilon/Cd3e antibody A02675 available?
N. Huang
Verified customer
Asked: 2015-02-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD3 epsilon/Cd3e antibody A02675 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-26
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CD3 epsilon/Cd3e antibody A02675. Please let me know if you require anything else.
G. Dhar
Verified customer
Asked: 2013-08-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-30
