Anti-CD34 Rabbit Monoclonal Antibody

Boster Bio Anti-CD34 Rabbit Monoclonal Antibody catalog # M00885-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Dog, Human, Mouse, Rat. Cited in 44 publication(s).

Product Info Summary

SKU: M00885-1
Size: 100 μl
Reactive Species: Dog, Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-CD34 Rabbit Monoclonal Antibody

See all CD34 products

SKU/Catalog Number

M00885-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD34 Rabbit Monoclonal Antibody catalog # M00885-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Dog, Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD34 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BBH-3

Immunogen

A synthesized peptide derived from human CD34

Reactive Species

M00885-1 is reactive to CD34 in Dog, Human, Mouse, Rat

Applications

M00885-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CD34 (Source: Uniprot.Org, NCBI)

Uniprot ID

P28906

Gene ID

947

Gene Name

CD34

Full Name

Hematopoietic progenitor cell antigen CD34

Weight

40.716kDa

Superfamily

CD34 family

Alternative Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 CD34 CD34 molecule hematopoietic progenitor cell antigen CD34|CD34 antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD34, check out the CD34 Infographic

CD34 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD34: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00885-1 has been cited in 44 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shuyuan Guo,Yusen Zhang,Yanmin Zhang,Fanhua Meng,Minghua Li,Zhendong Yu,Yun Chen,Guanghui Cui, "Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute
Species: Human,Rat
M00885-1 usage in article: APP:FLOW CYTOMETRY, SAMPLE:PBMC-5 CELL, DILUTION:NA

Peng L,Wang Y,Fei S,Wei C,Tong F,Wu G,Ma H,Dong X.The effect of combining Endostar with radiotherapy on blood vessels, tumor-associated macrophages, and T cells in brain metastases of Lewis lung cancer.Transl Lung Cancer Res.2020 Jun;9(3):745-760.doi:10.2
Species: Mouse
M00885-1 usage in article: APP:IF, SAMPLE:LUNG TUMOR TISSUE, DILUTION:NA

Liang C, Shangguan J, Yang L, Guo S. Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med. 2021 Jan;21(1):22. doi:10.3892/etm. 2020. 9454. Epub 2020 Nov 6. PMID: 33235631; PMCID:
Species: Human, Rat
M00885-1 usage in article: APP:IHC, SAMPLE:BRAIN AND TUMOR TISSUE, DILUTION:1:200

Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
M00885-1 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary Giovanna Di Emidio, et al. Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1389-1401. doi: 10.1016/j.bbadis.2019.02.011. Epub 2019 Feb 13.

Dl-3-n-Butylphthalide treatment enhances hemodynamics and ameliorates memory deficits in rats with chronic cerebral hypoperfusion

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase

Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters

Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1? expression in the transition zone

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device

Inhibitory effect of endostatin combined with paclitaxel-cisplatin on breast cancer in xenograft-bearing mice

Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Phosphoinositide 3-Kinase (PI3K) Subunit p110? Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse

Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1?

Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair

Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes

Modulating hair follicle size with?Wnt10b-DKK1?pair during hair regeneration

Li D, Wei X, Xie K, Chen K, Li J, Fang J. Br J Cancer. 2014 Jul 8;111(1):68-77. Doi: 10.1038/Bjc.2014.282. Epub 2014 May 29. A Novel Decoy Receptor Fusion Protein For Fgf-2 Potently Inhibits Tumour Growth.

Luo Bh, Xiong F, Wang Jp, Li Jh, Zhong M, Liu Ql, Luo Gq, Yang Xj, Xiao N, Xie B, Xiao H, Liu Rj, Dong Cs, Wang Ks, Wen Jf. Plos One. 2014 Jun 19;9(6):E99922. Doi: 10.1371/Journal.Pone.0099922. Ecollection 2014. Epidermal Growth Factor-Like Domain...

Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...

Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.

Qiu W, Lei M, Li J, Wang N, Lian X. Int J Med Sci. 2014 Jul 30;11(10):1022-8. Doi: 10.7150/Ijms.8383. Ecollection 2014. Activated Hair Follicle Stem Cells And Wnt/??-Catenin Signaling Involve In Pathnogenesis Of Sebaceous Neoplasms.

Zhang J, Yang W, Zhao D, Han Y, Liu B, Zhao H, Wang H, Zhang Q, Xu G. Oncol Lett. 2014 Jan;7(1):95-100. Epub 2013 Oct 29. Correlation Between Tsp-1, Tgf-?? And Ppar-?? Expression Levels And Glioma Microvascular Density.

Li Lx, Zhang Yl, Zhou L, Ke Ml, Chen Jm, Fu X, Ye Cl, Wu Jx, Liu Ry, Huang W. J Transl Med. 2013 Oct 14;11:257. Doi: 10.1186/1479-5876-11-257. Antitumor Efficacy Of A Recombinant Adenovirus Encoding Endostatin Combined With An E1B55Kd-Deficient Ad...

Yang J, Xiong Lj, Xu F, Zhao X, Liu B, Cai Kl, Wang Gb. Int J Endocrinol. 2013;2013:453898. Doi: 10.1155/2013/453898. Epub 2013 Nov 14. Estrogen Inhibits Colon Polyp Formation By Reducing Angiogenesis In A Carcinogen-Induced Rat Model.

Wang G, Xu D, Chai Q, Tan X, Zhang Y, Gu N, Tang J. Oncol Lett. 2014 May;7(5):1370-1374. Epub 2014 Feb 20. Magnetic Fluid Hyperthermia Inhibits The Growth Of Breast Carcinoma And Downregulates Vascular Endothelial Growth Factor Expression.

Zhu Sk, Zhou Y, Cheng C, Zhong S, Wu Hq, Wang B, Fan P, Xiong Jx, Yang Hj, Wu Hs. Mol Clin Oncol. 2014 Nov;2(6):973-981. Epub 2014 Jul 9. Overexpression Of Membrane-Type 2 Matrix Metalloproteinase Induced By Hypoxia-Inducible Factor-1?? In Pancrea...

Jing Z, Shi C, Zhu L, Xiang Y, Chen P, Xiong Z, Li W, Ruan Y, Huang L. J Cereb Blood Flow Metab. 2015 Aug;35(8):1249-59. Doi: 10.1038/Jcbfm.2015.55. Epub 2015 Apr 8. Chronic Cerebral Hypoperfusion Induces Vascular Plasticity And Hemodynamics But A...

Li J, Ye Y, Zhang R, Zhang L, Hu X, Han D, Chen J, He X, Wang G, Yang X, Wang L. Sci Rep. 2015 May 19;5:9720. Doi: 10.1038/Srep09720. Robo1/2 Regulate Follicle Atresia Through Manipulating Granulosa Cell Apoptosis In Mice.

Chen J, Jia Zy, Ma Zl, Wang Yy, Teng Gj. Plos One. 2011;6(6):E20790. Doi: 10.1371/Journal.Pone.0020790. Epub 2011 Jun 24. In Vivo Serial Mr Imaging Of Magnetically Labeled Endothelial Progenitor Cells Homing To The Endothelium Injured Artery In Mice.

Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...

Zhou J, Wang L, Xu X, Tu Y, Qin S, Yin Y. Oncol Lett. 2012 Nov;4(5):976-980. Epub 2012 Aug 8. Antitumor Activity Of Endostar Combined With Radiation Against Human Nasopharyngeal Carcinoma In Mouse Xenograft Models.

Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...

Huang Xf, Yuan Sj, Yang C. Mol Med Rep. 2012 Sep;6(3):547-52. Doi: 10.3892/Mmr.2012.974. Epub 2012 Jun 29. Effects Of Total Flavonoids From Drynaria??fortunei On The Proliferation And Osteogenic Differentiation Of Rat Dental Pulp Stem ...

Zhao Cl, Zhang Gp, Xiao Zz, Ma Zk, Lei Cp, Song Sy, Feng Yy, Zhao Yc, Feng Xs. J Breast Cancer. 2015 Jun;18(2):126-33. Doi: 10.4048/Jbc.2015.18.2.126. Epub 2015 Jun 26. Recombinant Human Granulocyte Colony-Stimulating Factor Promotes Preinvasive A...

Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.

Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...

Jiang Jt, Zhang Lf, Zhou B, Zhang Sq, Li Sm, Zhang W, Zhang J, Qiao Z, Kong Rr, Ma Yf, Chen S. Asian Pac J Cancer Prev. 2012;13(7):3379-83. Relationships Of Upa And Vegf Expression In Esophageal Cancer And Microvascular Density With Tumorous Invas...

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4 Customer Q&As for Anti-CD34 Rabbit Monoclonal Antibody

Question

We have seen staining in human megakaryoblast. What should we do? Is anti-CD34 Rabbit Monoclonal antibody supposed to stain megakaryoblast positively?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

Based on literature megakaryoblast does express CD34. Based on Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-09-16

Question

My boss were happy with the WB result of your anti-CD34 Rabbit Monoclonal antibody. However we have seen positive staining in placenta membrane using this antibody. Is that expected? Could you tell me where is CD34 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

Based on literature, placenta does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-27

Question

Our lab used your anti-CD34 Rabbit Monoclonal antibody for IHC on placenta in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining placenta as well as heart in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

I viewed the website and datasheets of our anti-CD34 Rabbit Monoclonal antibody and it appears that M00885-1 has been tested on mouse in both IHC and WB. Thus M00885-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-05

Question

We are currently using anti-CD34 Rabbit Monoclonal antibody M00885-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-27

Answer

The anti-CD34 Rabbit Monoclonal antibody (M00885-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-27

Size

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M00885-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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