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Product Info Summary
| SKU: | M00885-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD34 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00885-1
BM4082 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD34 Rabbit Monoclonal Antibody catalog # M00885-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD34 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BBH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD34
Reactive Species
M00885-1 is reactive to CD34 in Human, Mouse, Rat
Observed Molecular Weight
120 kDa
Calculated molecular weight
40.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00885-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: NIH/3T3 cell
IHC: human spleen tissue, human tonsil tissue
ICC/IF: HUVEC cell
Validation Images & Assay Conditions
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IHC analysis of CD34 using anti-CD34 antibody (M00885-1).
CD34 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD34 Antibody (M00885-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Western blot analysis of CD34 in expression NIH/3T3 cell lysate.
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IHC analysis of CD34 using anti-CD34 antibody (M00885-1).
CD34 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD34 Antibody (M00885-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Immunofluorescent analysis of HUVEC cells, using CD34 Antibody .
Specific Publications For Anti-CD34 Rabbit Monoclonal Antibody (M00885-1)
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4 Customer Q&As for Anti-CD34 Rabbit Monoclonal Antibody
Question
We have seen staining in human megakaryoblast. What should we do? Is anti-CD34 Rabbit Monoclonal antibody supposed to stain megakaryoblast positively?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Based on literature megakaryoblast does express CD34. Based on Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-16
Question
My boss were happy with the WB result of your anti-CD34 Rabbit Monoclonal antibody. However we have seen positive staining in placenta membrane using this antibody. Is that expected? Could you tell me where is CD34 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Based on literature, placenta does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-27
Question
Our lab used your anti-CD34 Rabbit Monoclonal antibody for IHC on placenta in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining placenta as well as heart in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
I viewed the website and datasheets of our anti-CD34 Rabbit Monoclonal antibody and it appears that M00885-1 has been tested on mouse in both IHC and WB. Thus M00885-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-05
Question
We are currently using anti-CD34 Rabbit Monoclonal antibody M00885-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
The anti-CD34 Rabbit Monoclonal antibody (M00885-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-27
