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Product Info Summary
| SKU: | A01022 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD35 CR1 Antibody
SKU/Catalog Number
A01022
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD35 CR1 Antibody catalog # A01022. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD35 CR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01022)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CR1/CR1L. AA range:300-350 & 740-790
Reactive Species
A01022 is reactive to CR1 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
223.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01022 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Western Blot analysis of A549 3T3 mouse-liver mouse-brain cells using CD35 Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD35 CR1 Antibody (A01022)
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Customer Q&As
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7 Customer Q&As for Anti-CD35 CR1 Antibody
Question
Is there a BSA free version of anti-CD35 antibody A01022 available?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD35 antibody A01022 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-20
Question
I have a question about product A01022, anti-CD35 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01022 anti-CD35 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-15
Question
See attached the WB image, lot number and protocol we used for blood using anti-CD35 antibody A01022. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Is this A01022 anti-CD35 antibody reactive to the isotypes of CR1?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The immunogen of A01022 anti-CD35 antibody is Synthesized peptide derived from CD35 at AA range: Internal. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-17
Question
Does A01022 anti-CD35 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Bhatt
Verified customer
Asked: 2017-09-22
Answer
As indicated on the product datasheet, A01022 anti-CD35 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-22
Question
I was wanting to use your anti-CD35 antibody for WB for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
B. Taylor
Verified customer
Asked: 2016-09-02
Answer
It shows on the product datasheet, A01022 anti-CD35 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-02
Question
Is a blocking peptide available for product anti-CD35 antibody (A01022)?
G. Gonzalez
Verified customer
Asked: 2014-08-27
Answer
We do provide the blocking peptide for product anti-CD35 antibody (A01022). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-08-27
