Human CD21/CR2 PicoKine™ Quick ELISA Kit

Human CD21/CR2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: FEK1315
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

Product Name

Human CD21/CR2 PicoKine™ Quick ELISA Kit

See all CR2 products

SKU/Catalog Number

FEK1315

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD21/CR2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CD21/CR2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1315)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Ile21-71

Sensitivity

<20 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1315 is reactive to CR2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1315 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK1315-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1315-STHuman CR2 Standard 2 50 ng/tube
FEK1315-CHuman CR2 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0140.0960.1680.3170.5650.9381.7572.382

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5926091358268861
Standard deviation3.3612.2249.304.4013.9355.96
CV(%)5.7%4.7%5.4%7.6%5.2%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CR2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11242146812731362133687.896.5%
Sample 279167621654868327229558.347.7%
Sample 324012232472646722418240361512.516.2%
*number of samples for each test n=16.

Gene/Protein Information For CR2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20023

Gene ID

1380

Gene Name

CR2

Full Name

Complement receptor type 2

Weight

112.916kDa

Superfamily

receptors of complement activation (RCA) family

Alternative Names

C3DR; C3DRSLEB9; CD21 antigen; CD21; Complement C3d receptor; complement component (3d/Epstein Barr virus) receptor 2; complement receptor type 2; CR2; EBV receptor; Epstein-Barr virus receptor CR2 C3DR, CD21, CR, CVID7, SLEB9 complement C3d receptor 2 complement receptor type 2|EBV receptor|complement component (3d/Epstein Barr virus) receptor 2|complement component 3d receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CR2, check out the CR2 Infographic

CR2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Human CD21/CR2 PicoKine™ Quick ELISA Kit

Question

Is the epitope of Human CD21/CR2 PicoKine intracellular?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Indeed, the epitope of this Human CD21/CR2 PicoKine antibody is intracellular, thus it is required to permeabilize the cells for flow cytometry.

Boster Scientific Support

Answered: 2019-05-31

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