Anti-CD4 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Western blot analysis of CD4 expression in THP-1 cell lysate.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD4 using anti-CD4 antibody (M00344).
CD4 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD4 Antibody (M00344) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Interaction between GZMK + CD8 + T cells and fibroblasts contributes to neutrophilic inflammation in nasal polyps. a Representative immunofluorescence staining of collagen I (COL1A1, green), CD8 (red), and GZMK (yellow) in NPs. The right image shows a greater magnification of the outlined area. b Spatial distribution analysis of GZMK + CD8 + T and COL1A1 + cells in the same tissue field demonstrated in ( a ) using HALO software. c–d The number of COL1A1 + fibroblasts within a radius of 25 μm from the nuclear center of GZMK + CD8 + T, GZMB + CD8 + T, CD4 + T, or CD19 + B cells in CIT group (left, n = 10 samples) and NP group (right, n = 10 samples) ( c ). Average distance from the indicated cell types to the closest COL1A1 + fibroblasts in CIT group (left, n = 10 samples) and NP group (right, n = 10 samples) ( d ). e DEGs between NP-derived primary fibroblasts (NPDF) treated with and without recombinant human GZMK ( n = 4). Two-sided Wald test (default for DESeq2 r-package) was used for differential expression analysis utilizing standard cutoffs of