Anti-CD40L/Cd40lg Antibody Picoband™

CD40 Ligand/TNFSF5 antibody

Boster Bio Anti-CD40L/Cd40lg Antibody Picoband™ catalog # A01114-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01114-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-CD40L/Cd40lg Antibody Picoband™

View all CD40 Ligand/TNFSF5 Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-CD40L/Cd40lg Antibody Picoband™ catalog # A01114-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD40L/Cd40lg Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01114-2)




Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.




Rabbit IgG


E. coli-derived mouse CD40L recombinant protein (Position: E107-L260).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.


No cross-reactivity with other proteins.

Reactive Species

A01114-2 is reactive to Cd40lg in Human, Mouse, Rat


A01114-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of CD40 Ligand/TNFSF5

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Cd40lg (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

CD40 ligand




tumor necrosis factor family

Alternative Names

CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 Cd40lg|CD154, CD40-L, Cd40, Cd40l, HIGM, HIGM1, IGM, IMD, IMD3, Ly-6, Ly-62, Ly62, T-BA, T-BAM, TRAP, Tnfs, Tnfsf5, Tnlg8b, gp3, gp39|CD40 ligand|CD40 ligand|T-cell antigen Gp39|TNF-related activation protein|tumor necrosis factor ligand 8b|tumor necrosis factor ligand superfamily member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd40lg, check out the Cd40lg Infographic

Cd40lg infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd40lg: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A01114-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, Lin Q, Lin R. Evid Based Complement Alternat Med. 2011;2011:930324. Doi: 10.1093/Ecam/Nep013. Epub 2011 Jun 5. Protective Effects Of Chinese Traditional Medicine Buyang Huanwu Decoction On Myocardial In...

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5 Customer Q&As for Anti-CD40L/Cd40lg Antibody Picoband™


Our lab were content with the WB result of your anti-CD40L/Cd40lg antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CD40LG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-26


From what I have seen in literature, blood does express CD40LG. Generally CD40LG expresses in cell membrane. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-26


I was wanting to use using your anti-CD40L/Cd40lg antibody for r-mmu-198933; immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-17


We appreciate your inquiry. This A01114-2 anti-CD40L/Cd40lg antibody is validated on rat spleen tissue, mouse spleen tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-17


We bought anti-CD40L/Cd40lg antibody for WB on blood a few years ago. I am using rat, and We want to use the antibody for IHC next. Our lab want to know about examining blood as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

B. Williams

Verified customer

Asked: 2015-02-06


I have checked the website and datasheets of our anti-CD40L/Cd40lg antibody and I see that A01114-2 has been tested on rat in both WB and IHC. Thus A01114-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website to find out more information about them.

Boster Scientific Support

Answered: 2015-02-06


We have observed staining in human blood. Are there any suggestions? Is anti-CD40L/Cd40lg antibody supposed to stain blood positively?

A. Singh

Verified customer

Asked: 2013-05-30


From what I have seen in literature blood does express CD40LG. From what I have seen in, CD40LG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-05-30


We are currently using anti-CD40L/Cd40lg antibody A01114-2 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

B. Jones

Verified customer

Asked: 2013-03-08


The anti-CD40L/Cd40lg antibody (A01114-2) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-03-08



Total: $315


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