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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd40lg in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0574)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E61-L260
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0574 is reactive to Cd40lg in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0574 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD40 Ligand/TNFSF5
CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
|EK0574-CAP||Anti-Mouse Cd40lg Pre-coated 96-well strip microplate||1|
|EK0574-ST||Mouse Cd40lg Standard||2 vials, 4 ng/tube|
|EK0574-DA||Mouse Cd40lg Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CD40L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD40 Ligand/TNFSF5, Dilution ratio of 1:1, concentration in serum is around 10pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD40 Ligand/TNFSF5 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cd40lg (Source: Uniprot.Org, NCBI)
tumor necrosis factor family
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 Cd40lg|CD154, CD40-L, Cd40, Cd40l, HIGM, HIGM1, IGM, IMD, IMD3, Ly-6, Ly-62, Ly62, T-BA, T-BAM, TRAP, Tnfs, Tnfsf5, Tnlg8b, gp3, gp39|CD40 ligand|CD40 ligand|T-cell antigen Gp39|TNF-related activation protein|tumor necrosis factor ligand 8b|tumor necrosis factor ligand superfamily member 5*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd40lg, check out the Cd40lg Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd40lg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0574 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice
Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan): Part IV. Immunomodulatory activity in vivo
Ganoderma atrum polysaccharide ameliorates intestinal mucosal dysfunction associated with autophagy in immunosuppressed mice
Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...
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