Anti-Macrosialin CD68 Antibody

CD68/SR-D1 antibody

Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA2018. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PA2018
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Macrosialin CD68 Antibody

View all CD68/SR-D1 Antibodies

SKU/Catalog Number

PA2018

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA2018. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrosialin CD68 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2018)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD68.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2018 is reactive to CD68 in Human

Applications

PA2018 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

37.408kDa

Background of CD68/SR-D1

CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD68 (Source: Uniprot.org, NCBI)

Gene Name

CD68

Full Name

Macrosialin

Weight

37.408kDa

Superfamily

LAMP family

Alternative Names

CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1 CD68 GP110, LAMP4, SCARD1 CD68 molecule macrosialin|CD68 antigen|macrophage antigen CD68|scavenger receptor class D, member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD68, check out the CD68 Infographic

CD68 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD68: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2018 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jian Pang,Li Zhou,Liqiu Liao et al.Correlation of sTILs Infiltration and UBR5 Expression on the Efficacy of Neoadjuvant Chemotherapy Efficacy and Prognosis in Early Triple Negative Breast Cancer, 13 January 2021, PREPRINT (Version 1) available at Research
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:NA

Fan Guo,Wei na Kong,Gang Zhao et al.The Correlation Between Tumor-associated Macrophage Infiltration and Progression in Cervical Carcinoma, 01 December 2020, PREPRINT (Version 1) available at Research Square[+https://doi.org/10.21203/rs.3.rs-115339/v1+]
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:CERVICAL TISSUE, DILUTION:1:50

Guo F,Feng YC,Zhao G,Zhang R,Cheng ZZ,Kong WN,Wu HL,Xu B,Lv X,Ma XM.Tumor-Associated CD163+ M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer.Cancer Manag Res.2020 Jul 15;12:5831-5843.doi:10.2147/CMAR.S257692.PMID:32
Species: Human
PA2018 usage in article: APP:IHC, SAMPLE:SQUAMOUS CELLS, DILUTION:1:50

Targeting Src attenuates peritoneal fibrosis and inhibits the epithelial to mesenchymal transition

LDL Receptor Gene-ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia With Dominant Inheritance and Diet-induced Coronary Atherosclerosis

Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization

Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice

Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation

Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

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16 Customer Q&As for Anti-Macrosialin CD68 Antibody

Question

We are currently using anti-CD68 antibody PA2018 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The anti-CD68 antibody (PA2018) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-08

Question

Do you have a BSA free version of anti-CD68 antibody PA2018 available?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD68 antibody PA2018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-11

Question

We were happy with the WB result of your anti-CD68 antibody. However we have seen positive staining in skin isoform short: cell membrane using this antibody. Is that expected? Could you tell me where is CD68 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

Based on literature, skin does express CD68. Generally CD68 expresses in isoform short: cell membrane. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-28

Question

I see that the anti-CD68 antibody PA2018 works with IHC, what is the protocol used to produce the result images on the product page?

L. Collins

Verified customer

Asked: 2019-06-12

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-12

Question

Is this PA2018 anti-CD68 antibody reactive to the isotypes of CD68?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

The immunogen of PA2018 anti-CD68 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD68(341-354aa FCIIRRRPSAYQAL). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-05

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-CD68 antibody PA2018. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-29

Question

I have a question about product PA2018, anti-CD68 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2018 anti-CD68 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-15

Question

Our lab used your anti-CD68 antibody for WB on liver a few months ago. I am using human, and I plan to use the antibody for IHC next. I am interested in examining liver as well as spleen umbilical cord blood in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-06-13

Answer

I looked at the website and datasheets of our anti-CD68 antibody and I see that PA2018 has been tested on human in both WB and IHC. Thus PA2018 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-06-13

Question

Is a blocking peptide available for product anti-CD68 antibody (PA2018)?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

We do provide the blocking peptide for product anti-CD68 antibody (PA2018). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-29

Question

Does PA2018 anti-CD68 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Edwards

Verified customer

Asked: 2017-10-26

Answer

You can see on the product datasheet, PA2018 anti-CD68 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-26

Question

My lab would like using your anti-CD68 antibody for negative regulation of dendritic cell antigen processing and presentation studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

E. Dhar

Verified customer

Asked: 2016-12-26

Answer

I appreciate your inquiry. This PA2018 anti-CD68 antibody is tested on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-12-26

Question

We want to test anti-CD68 antibody PA2018 on human adipose tissue for research purposes, then I may be interested in using anti-CD68 antibody PA2018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Jones

Verified customer

Asked: 2016-01-21

Answer

The products we sell, including anti-CD68 antibody PA2018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-01-21

Question

Will anti-CD68 antibody PA2018 work for IHC with adipose tissue?

J. Parker

Verified customer

Asked: 2016-01-12

Answer

According to the expression profile of adipose tissue, CD68 is highly expressed in adipose tissue. So, it is likely that anti-CD68 antibody PA2018 will work for IHC with adipose tissue.

Boster Scientific Support

Answered: 2016-01-12

Question

See attached the WB image, lot number and protocol we used for adipose tissue using anti-CD68 antibody PA2018. Please let me know if you require anything else.

K. Walker

Verified customer

Asked: 2015-12-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-15

Question

I was wanting to use your anti-CD68 antibody for IHC for human adipose tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adipose tissue identification?

D. Edwards

Verified customer

Asked: 2015-01-26

Answer

It shows on the product datasheet, PA2018 anti-CD68 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adipose tissue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-01-26

Question

We have seen staining in human liver. Are there any suggestions? Is anti-CD68 antibody supposed to stain liver positively?

J. Anderson

Verified customer

Asked: 2014-02-17

Answer

From literature liver does express CD68. From Uniprot.org, CD68 is expressed in leukocyte, spleen umbilical cord blood, adipose tissue, skin, platelet, liver, among other tissues. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-02-17

Order DetailsPrice
PA2018

100μg

$370
PA2018-10ug

10μg sample (liquid)

$99
PA2018-Biotin

100 μg Biotin conjugated

$570
PA2018-Cy3

100 μg Cy3 conjugated

$570
PA2018-Dylight488

100 μg Dylight488 conjugated

$570
PA2018-Dylight550

100 μg Dylight550 conjugated

$570
PA2018-Dylight594

100 μg Dylight594 conjugated

$570
PA2018-FITC

100 μg FITC conjugated

$570
PA2018-HRP

100 μg HRP conjugated

$570
PA2018-APC

100 μg APC conjugated

$670
PA2018-PE

100 μg PE conjugated

$670
PA2018-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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