Anti-Macrosialin CD68 Antibody

CD68/SR-D1 antibody

Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA1518. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat. Cited in 56 publication(s).

Product Info Summary

SKU: PA1518
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, WB

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Product Name

Anti-Macrosialin CD68 Antibody

View all CD68/SR-D1 Antibodies

SKU/Catalog Number

PA1518

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA1518. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrosialin CD68 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1518)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse CD68 (312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1518 is reactive to Cd68 in Mouse, Rat

Applications

PA1518 is guaranteed for IF, IHC, IHC-F, WB Boster Guarantee

Background of CD68/SR-D1

CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells. CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be directed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Immunofluorescence, 2μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Cd68 (Source: Uniprot.Org, NCBI)

Uniprot ID

P31996

Gene ID

12514

Gene Name

Cd68

Full Name

Macrosialin

Weight

34.818kDa

Superfamily

LAMP family

Alternative Names

CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1 Cd68|La, Lamp4, Sca, Scard1, gp11, gp110, ma|CD68 antigen|macrosialin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd68, check out the Cd68 Infographic

Cd68 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd68: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1518 has been cited in 56 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HPMA–Copolymer Nanocarrier Targets Tumor-Associated Macrophages in Primary and Metastatic Breast Cancer

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression
Species: Mouse

A somatic mutation in erythro-myeloid progenitors causes neurodegenerative disease
Species: Mouse

Meng B,Li Y,Ding Y,Xu X,Wang L,Guo B,Zhu B,Zhang J,Xiang L,Dong J,Liu M,Xiang L,Xiang G.Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.Sci Adv.2021 May 21;7(21):eabe6903.doi:10.1126/sciadv.abe6903.PMID:34020949.
Species: Mouse
PA1518 usage in article: APP:IHC, SAMPLE:AORTIC TISSUE, DILUTION:1:200

Guizhi Yang,Shengwen Li,Jiahua Jin,Yuanyuan Xuan,Liqin Ding,Minxia Huang,Jun Liu,Biye Wang,Tian Lan,Protective effects of Longhu Rendan on chronic liver injury and fibrosis in mice,Liver Research,2021,,ISSN 2542-5684,https://doi.org/10.1016/j.livres.2021.05.002.
Species: Mouse
PA1518 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000

Zhou Y,Zhao B,Zhang XL,Lu YJ,Lu ST,Cheng J,Fu Y,Lin L,Zhang NY,Li PX,Zhang J,Zhang J.Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration.Stem Cell Res Ther.2021 May 1;12(1):257.doi:10.1186/s13287-021-02287-9.PMID:33933157;PMCID:PMC8088044.
Species: Human,Mouse
PA1518 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:200

Yonghui Hou,Jiyao Luan,Tiancheng Deng et al.Tauroursodeoxycholic Acid Alleviates Secondary Injury in Spinal Cord Injury Mice Through Reducing Oxidative Stress, Apoptosis, and Inflammatory Response,07 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-361252/ v1]
Species: Mouse
PA1518 usage in article: APP:IF, SAMPLE:CORTICAL NEURONS, DILUTION:1:300

Ma Y,Wei C,Qi X,Pu Y,Dong L,Xu L,Zhou S,Zhu J,Chen X,Wang X,Su C.Schistosoma japonicum-derived peptide SJMHE1 promotes peripheral nerve repair through a macrophage-dependent mechanism.Am J Transl Res.2021 Mar 15;13(3):1290-1306.PMID:33841657;PMCID:PMC8014408.
Species: Mouse,Rat
PA1518 usage in article: APP:IF, SAMPLE:NERVE CONDUITS, DILUTION:1:200

Shi D,Wu J,Wu Y,Lin X,Xu C,Lian X. High-Fat Diet-Related Obesity Promotes Urethane-Induced Lung Tumorigenesis in C57BL/6J Mice. Front Oncol.2021 Feb 23;11:620993.doi:10.3389/fonc.2021. 620993.PMID:33708630;PMCID:PMC7942226.
Species: Human,Mouse
PA1518 usage in article: APP:IHC, SAMPLE:TRACHEA, DILUTION:1:100

Shi D,Wu J,Wu Y,Lin X,Xu C,Lian X. High-Fat Diet-Related Obesity Promotes Urethane-Induced Lung Tumorigenesis in C57BL/6J Mice. Front Oncol.2021 Feb 23;11:620993.doi:10.3389/fonc.2021. 620993.PMID:33708630;PMCID:PMC7942226.
Species: Human,Mouse
PA1518 usage in article: APP:IHC, SAMPLE:TRACHEA, DILUTION:1:100

Han X,Chen X,Chen S,Luo Q,Liu X,He A,He S,Qiu J,Chen P,Wu Y,Zhuang J,Yang M,Wu C,Wu N,Yang Y,Ge J,Zhuang J,Yu K.Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway
Species: Rat
PA1518 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:1:200

Yanmin Cui,Yang Lin,Xianjun Meng,Jingxuan Ma,Haotian Deng,Xia Liu,Xinyuan He,Jin Zhao,Cyanidin-3-galactoside from Aronia melanocarpa ameliorates PM10 induced pulmonary injury by modulating M1/M2 macrophage polarization and NRF2/Sirt1 MAPK signaling,Journa
Species: Mouse
PA1518 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA

Masahiro Shindo,Masatomo Maeda,Ko Myat et al.LDH-A Knockdown: Changes in The LDH Isoenzyme Profile and Variability in Glioma Response,08 January 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-141065/v1]
Species: Mouse
PA1518 usage in article: APP:IHC, SAMPLE:SUBCUTANEOUS TUMOR TISSUE, DILUTION:NA

Shuai Wu,Wei Yuan,Weiwei Luo et al.MiR-126 Suppresses Colitis-Associated Colorectal Cancer by Regulating Crosstalk Between Epithelium Cells and Macrophages via CXCL12, 28 December 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10
Species: Mouse,Human
PA1518 usage in article: APP:IHC, SAMPLE:COLON TISSUE, DILUTION:NA

Cui M,Liang J,Xu D,Zhao L,Zhang X,Zhang L,Ren S,Liu D,Niu X,Zang YJ,Zhang B. NLRP3 inflammasome is involved in nerve recovery after sciatic nerve injury. Int Immunopharmacol.2020 Jul;84:106492.doi:10.1016/j.intimp.2020.106492.Epub 2020 May 18.PMID:3240294
Species: Mouse
PA1518 usage in article: APP:IF, SAMPLE:NERVE TISSUE, DILUTION:1:200

Ye Z,Huang S,Zhang Y,Mei X,Zheng H,Li M,Chen J,Lu F.Galectins, Eosinophiles, and Macrophages May Contribute to Schistosoma japonicum Egg-Induced Immunopathology in a Mouse Model.Front Immunol.2020 Mar 13;11:146.doi:10.3389/fimmu.2020.00146.PMID:32231658;
Species: Mouse
PA1518 usage in article: APP:IHC, SAMPLE:LIVER,SPLEEN AND INTESTINE TISSUE, DILUTION:1:200

Chen C,Wang Y,Cao Y,Wang Q,Anwaier G,Zhang Q,Qi R.Mechanisms underlying the inhibitory effects of probucol on elastase-induced abdominal aortic aneurysm in mice.Br J Pharmacol.2020 Jan;177(1):204-216.doi: 10.1111/bph.14857.Epub 2019 Nov 3.PMID:31478560;PM
Species: Mouse,Rat
PA1518 usage in article: APP:IHC, SAMPLE:AORTIC TISSUE, DILUTION:1:100

Qi Y,Si D,Zhu L,Qi Y,Wu Z,Chen D,Yang Y.High-fat diet-induced obesity affects alpha 7 nicotine acetylcholine receptor expressions in mouse lung myeloid cells.Sci Rep.2020 Oct 27;10(1):18368.doi:10.1038/s41598-020-75414-w.PMID:33110180;PMCID:PMC7592050.
Species: Rat
PA1518 usage in article: APP:IF, SAMPLE:LUNG TISSUE, DILUTION:NA

GPR120 is an important inflammatory regulator in the development of osteoarthritis

Macrophage-stimulated microRNA expression in mural cells promotes transplantation-induced neointima formation

Inhibition of poly (ADP-ribose) polymerase-1 protects chronic alcoholic liver injury

UPLC/Q-TOFMS-Based Metabolomics Approach to Reveal the Protective Role of Other Herbs in An-Gong-Niu-Huang Wan Against the Hepatorenal Toxicity of %u2026

Targeting Src attenuates peritoneal fibrosis and inhibits the epithelial to mesenchymal transition

LDL Receptor Gene-ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia With Dominant Inheritance and Diet-induced Coronary Atherosclerosis

Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization

Low dose doxycycline decreases systemic inflammation and improves glycemic control, lipid profiles, and islet morphology and function in db/db mice

Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation

Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration

Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm

Renin-angiotensin system activation accelerates atherosclerosis in experimental renal failure by promoting endoplasmic reticulum stress-related inflammation

Blockage of Galectin-receptor Interactions by ?-lactose Exacerbates Plasmodium berghei-induced Pulmonary Immunopathology

Inhibition of EGF Receptor Blocks the Development and Progression of Peritoneal Fibrosis.

CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation

1,25(OH)2D3 downregulates the Toll-like receptor 4-mediated inflammatory pathway and ameliorates liver injury in diabetic rats

PTEN inhibits macrophage polarization from M1 to M2 through CCL2 and VEGF-A reduction and NHERF-1 synergism

Influence of Kupffer cells on hepatic signal transduction as demonstrated by second messengers and nuclear transcription factors

Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation

SIV Infection Impairs the Central Nervous System in Chinese Rhesus Macaques

Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-?B Pathway

Leftin A, Zhao H, Turkekul M, de Stanchina E, Manova K, Koutcher JA. Sci Rep. 2017 Sep 14;7(1):11632. doi: 10.1038/s41598-017-11899-2. Iron deposition is associated with differential macrophage infiltration and therapeutic response to iron chelati...

Qu Q, Bing W, Meng X, Xi J, Bai X, Liu Q, Guo Y, Zhao X, Bi Y. Oncotarget. 2017 Nov 3;8(63):106790-106806. doi: 10.18632/oncotarget.22365. eCollection 2017 Dec 5. Upregulation of miR-126-3p promotes human saphenous vein endothelial cell proliferat...

Lin L, Li R, Cai M, Huang J, Huang W, Guo Y, Yang L, Yang G, Lan T, Zhu K. Oxid Med Cell Longev. 2018 Mar 18;2018:7808656. doi: 10.1155/2018/7808656. eCollection 2018. Andrographolide Ameliorates Liver Fibrosis in Mice: Involvement of TLR4/NF-κB a...

Huang L, Zhang K, Guo Y, Huang F, Yang K, Chen L, Huang K, Zhang F, Long Q, Yang Q. Sci Rep. 2017 Sep 20;7(1):11989. doi: 10.1038/s41598-017-12095-y. Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mous...

Leftin A, Ben-Chetrit N, Klemm F, Joyce JA, Koutcher JA. PLoS One. 2017 Sep 12;12(9):e0184765. doi: 10.1371/journal.pone.0184765. eCollection 2017. Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer

Pang Y, Zhang Y, Huang L, Xu L, Wang K, Wang D, Guan L, Zhang Y, Yu F, Chen Z, Xie X. Int J Mol Sci. 2017 Dec 19;18(12). pii: E2766. doi: 10.3390/ijms18122766. Effects and Mechanisms of Total Flavonoids from Blumea balsamifera (L.) DC. on Skin Wou...

Guo, X., Liao, J., Huang, X., Wang, Y., Huang, W., & Liu, G. (2017). Reversal of adipose tissue loss by probucol in mice with deficiency of both scavenger receptor class B type 1 and LDL receptor on high fat diet. Biochemical and Biophysical Resea...

Gu X, Wu G, Yao Y, Zeng J, Shi D, Lv T, Luo L, Song Y. Free Radic Biol Med. 2015 Jun;83:149-58. Doi: 10.1016/J.Freeradbiomed.2015.02.034. Epub 2015 Mar 13. Intratracheal Administration Of Mitochondrial Dna Directly Provokes Lung Inflammation Throu...

Wang W, Li X, Zheng D, Zhang D, Peng X, Zhang X, Ai F, Wang X, Ma J, Xiong W, Li G, Zhou Y, Shen S. Mol Med Rep. 2015 Apr;11(4):2397-406. Doi: 10.3892/Mmr.2014.3018. Epub 2014 Nov 28. Dynamic Changes And Functions Of Macrophages And M1/M2 Subpopul...

Pan X, Wang P, Luo J, Wang Z, Song Y, Ye J, Hou X. Endocrine. 2015 Apr;48(3):834-47. Doi: 10.1007/S12020-014-0384-X. Epub 2014 Aug 20. Adipogenic Changes Of Hepatocytes In A High-Fat Diet-Induced Fatty Liver Mice Model And Non-Alcoholic Fatty Live...

Zhang Jx, Wu Hs, Wang H, Zhang Jh, Wang Y, Zheng Qc. World J Gastroenterol. 2005 Jul 28;11(28):4423-6. Protection Against Hepatic Ischemia/Reperfusion Injury Via Downregulation Of Toll-Like Receptor 2 Expression By Inhibition Of Kupffer Cell Funct...

Liu B, Luo Xj, Yang Zb, Zhang Jj, Li Tb, Zhang Xj, Ma Ql, Zhang Gg, Hu Cp, Peng J. J Cardiovasc Pharmacol. 2014 Jun;63(6):567-76. Doi: 10.1097/Fjc.0000000000000082. Inhibition Of Nox/Vpo1 Pathway And Inflammatory Reaction By Trimethoxystilbene In ...

Zheng T,, Yang X,, Wu D,, Xing S,, Bian F,, Li W,, Chi J,, Bai X,, Wu G,, Chen X,, Zhang Y,, Jin S,,. Br J Pharmacol. 2015 Jul;172(13):3284-301. Doi: 10.1111/Bph.13120. Epub 2015 Apr 24. Salidroside Ameliorates Insulin Resistance Through Activatio...

He W, Lv Y, Zhao Y, Xu C, Jin Z, Qin C, Yin L. Int J Pharm. 2015 Apr 30;484(1-2):163-71. Doi: 10.1016/J.Ijpharm.2015.02.053. Epub 2015 Feb 24. Core-Shell Structured Gel-Nanocarriers For Sustained Drug Release And Enhanced Antitumor Effect.

Liu Z,, Xu S,, Huang X, Wang J, Gao S, Li H, Zhou C, Ye J, Chen S, Jin Zg, Liu P. Br J Pharmacol. 2015 Dec;172(23):5661-75. Doi: 10.1111/Bph.13068. Epub 2015 Feb 27. Cryptotanshinone, An Orally Bioactive Herbal Compound From Danshen, Attenuates At...

Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, Wang X, Altaf A, Wang L, He D, Wang H, Qu P. Int J Mol Med. 2015 May;35(5):1179-88. Doi: 10.3892/Ijmm.2015.2129. Epub 2015 Mar 9. P2X7R Is Involved In The Progression Of Atherosclerosis By Promoting Nlr...

Xiang S, Liu J, Dong N, Shi J, Xiao Y, Wang Y, Hu X, Gong L, Wang W. J Vasc Res. 2014;51(2):132-43. Doi: 10.1159/000355193. Epub 2014 Mar 28. Suppressor Of Cytokine Signaling 3 Is A Negative Regulator For Neointimal Hyperplasia Of Vein Graft Steno...

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10 Customer Q&As for Anti-Macrosialin CD68 Antibody

Question

What Are CD68 Macrophages?

Verified customer

Asked: 2021-10-20

Answer

CD68 is a protein highly expressed by cells in the monocyte lineage by circulating macrophages, and by tissue macrophages such as Kupffer cells, microglia.

Boster Scientific Support

Answered: 2021-10-20

Question

Our team were content with the WB result of your anti-CD68 antibody. However we have seen positive staining in leukocyte isoform short: cell membrane using this antibody. Is that expected? Could you tell me where is CD68 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

According to literature, leukocyte does express CD68. Generally CD68 expresses in isoform short: cell membrane. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-10

Question

What tissue types is PA1518 known to stain for?

Verified customer

Asked: 2019-12-04

Answer

The Anti-Macrosialin CD68 Antibody (PA1518) stains the following tissue types: Macrophage Dendritic cells Brain Spleen Mammary gland

Boster Scientific Support

Answered: 2019-12-04

Question

We have seen staining in mouse adipose tissue. What should we do? Is anti-CD68 antibody supposed to stain adipose tissue positively?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

From what I have seen in literature adipose tissue does express CD68. From what I have seen in Uniprot.org, CD68 is expressed in leukocyte, spleen umbilical cord blood, adipose tissue, skin, platelet, liver, among other tissues. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-12-03

Question

Which amino acid range is the immunogen sequence of PA1518 from?

Verified customer

Asked: 2019-11-29

Answer

For the Anti-Macrosialin CD68 Antibody (PA1518), a synthetic peptide corresponding to a sequence in the middle region of mouse CD68 (312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.

Boster Scientific Support

Answered: 2019-12-02

Question

Has this antibody been tested for cross-reactivity with human samples in IHC or Western Blot?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

We tested human samples and got negative results in IHC and WB.

Boster Scientific Support

Answered: 2019-11-29

Question

Do you have an IHC application on Ventana machine for PA1518?

Verified customer

Asked: 2019-07-03

Answer

Our lab hasn't used the Ventana machine for our antibodies. We suggest to watch some on-line vedio or read some related articles. http://www.path.queensu.ca/qlmp/sites/webpublish.queensu.ca.qlmpwww/files/files/Ventana%20Pamphlet.pdf

Boster Scientific Support

Answered: 2019-07-05

Question

Our lab used your anti-CD68 antibody for WB on spleen umbilical cord blood in the past. I am using mouse, and I plan to use the antibody for IF next. My lab would like examining spleen umbilical cord blood as well as adipose tissue in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

I looked at the website and datasheets of our anti-CD68 antibody and it seems that PA1518 has been validated on mouse in both WB and IF. Thus PA1518 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-05-18

Question

Our lab want to know about using your anti-CD68 antibody for autocrine signaling studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

R. Evans

Verified customer

Asked: 2016-09-23

Answer

We appreciate your inquiry. This PA1518 anti-CD68 antibody is tested on rat spleen tissue, tissue lysate, mouse spleen tissue. It is guaranteed to work for IF, IHC-P, IHC-F, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-09-23

Question

We are currently using anti-CD68 antibody PA1518 for rat tissue, and we are satisfied with the IHC-F results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

K. Carter

Verified customer

Asked: 2016-01-08

Answer

The anti-CD68 antibody (PA1518) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-08

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:PA1518

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PA1518
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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